##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548112_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2197211
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.454357819981787	31.0	31.0	33.0	30.0	34.0
2	31.840092280623026	31.0	31.0	34.0	30.0	34.0
3	31.89581519480833	33.0	31.0	34.0	30.0	34.0
4	35.63677908038873	37.0	35.0	37.0	33.0	37.0
5	35.536359503024514	37.0	35.0	37.0	33.0	37.0
6	35.60151118850215	37.0	35.0	37.0	33.0	37.0
7	36.0394422747747	37.0	35.0	37.0	35.0	37.0
8	36.084126194525695	37.0	35.0	37.0	35.0	37.0
9	37.92348481779857	39.0	38.0	39.0	35.0	39.0
10	37.27538911829588	39.0	37.0	39.0	34.0	39.0
11	36.860116301984654	39.0	37.0	39.0	32.0	39.0
12	36.409988844949346	38.0	35.0	39.0	32.0	39.0
13	36.198816590668805	39.0	35.0	39.0	32.0	39.0
14	37.13704555456895	39.0	35.0	41.0	31.0	41.0
15	37.36963769069061	39.0	35.0	41.0	32.0	41.0
16	37.49416874392127	39.0	35.0	41.0	32.0	41.0
17	37.445498406843946	39.0	35.0	41.0	32.0	41.0
18	37.371570140509945	39.0	36.0	41.0	32.0	41.0
19	37.32673147913423	39.0	35.0	41.0	32.0	41.0
20	37.16559310871828	39.0	35.0	41.0	32.0	41.0
21	37.052186612937945	39.0	35.0	41.0	32.0	41.0
22	37.0239899581788	39.0	35.0	41.0	32.0	41.0
23	36.948025474112406	39.0	35.0	41.0	32.0	41.0
24	36.960804401580006	39.0	35.0	41.0	32.0	41.0
25	36.94792989840302	39.0	35.0	41.0	32.0	41.0
26	36.83550419144998	39.0	35.0	41.0	31.0	41.0
27	36.79336258556871	39.0	35.0	41.0	31.0	41.0
28	36.75356212944501	38.0	35.0	41.0	31.0	41.0
29	36.686723305135466	38.0	35.0	41.0	31.0	41.0
30	36.58425021538669	38.0	35.0	41.0	31.0	41.0
31	36.49272373021981	38.0	35.0	41.0	30.0	41.0
32	36.38774291590566	38.0	35.0	41.0	30.0	41.0
33	36.296700680999685	38.0	35.0	41.0	30.0	41.0
34	36.16547250127548	38.0	35.0	41.0	30.0	41.0
35	36.07326924906165	38.0	35.0	41.0	30.0	41.0
36	35.914141609522254	38.0	35.0	41.0	29.0	41.0
37	35.837675580542786	38.0	35.0	40.0	29.0	41.0
38	35.69590130397126	38.0	35.0	40.0	28.0	41.0
39	35.617693976591234	38.0	35.0	40.0	27.0	41.0
40	35.478720068304774	38.0	35.0	40.0	27.0	41.0
41	35.41132690488078	38.0	34.0	40.0	27.0	41.0
42	35.38774382615051	38.0	34.0	40.0	27.0	41.0
43	35.30009179819326	38.0	34.0	40.0	26.0	41.0
44	35.14034701264467	38.0	34.0	40.0	26.0	41.0
45	35.13650350376	38.0	34.0	40.0	26.0	41.0
46	35.07762522579761	38.0	34.0	40.0	26.0	41.0
47	34.963227473374204	38.0	34.0	40.0	25.0	41.0
48	34.8486276465938	37.0	34.0	40.0	24.0	41.0
49	34.73549103841188	37.0	34.0	40.0	24.0	41.0
50	34.5638047506589	37.0	34.0	40.0	24.0	41.0
51	34.21784935538735	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	1.0
13	3.0
14	11.0
15	48.0
16	128.0
17	343.0
18	778.0
19	1564.0
20	2758.0
21	4560.0
22	6994.0
23	10468.0
24	15563.0
25	22237.0
26	29566.0
27	35105.0
28	38939.0
29	44285.0
30	52774.0
31	64170.0
32	79795.0
33	102406.0
34	162016.0
35	234462.0
36	155159.0
37	202532.0
38	314921.0
39	615517.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.13173427586153	24.513257943820598	30.70624532646159	12.648762453856275
2	29.354395185532933	26.159845367604657	31.011723498562493	13.474035948299912
3	28.672712816384045	25.274586737459444	32.93552599181417	13.117174454342345
4	25.655342158763993	27.138540631737236	32.67810874786263	14.528008461636139
5	23.330986418691698	31.85074169026097	30.678846956437045	14.139424934610286
6	21.43553805255845	40.641613390794056	27.318086428658876	10.604762127988618
7	89.9341483362317	2.6053483256728645	6.012303779655208	1.4481995584402225
8	91.00295784064436	1.7215915995323163	5.61006657985965	1.6653839799636903
9	86.44964002091743	4.523871398786917	6.814548079360608	2.2119405009350492
10	42.86884600523118	36.03977952049212	12.188224071334067	8.90315040294264
11	30.230460342679876	23.616484716306264	28.850711197058455	17.302343743955404
12	31.45765245122112	22.171379990360506	28.13070751966925	18.240260038749124
13	24.36479700857132	27.57341010945239	29.658098380173776	18.403694501802512
14	16.860237819672303	31.788298893460848	31.013498476022555	20.33796481084429
15	15.830159233683064	29.892577453872203	37.86204420057974	16.415219111864996
16	22.043354052023222	27.19024253929186	34.602958022693315	16.163445385991604
17	21.979773449158955	26.923631822342053	27.37292868095053	23.723666047548463
18	22.08645414573293	28.184685039352157	31.784930987510982	17.943929827403924
19	25.20017422086454	29.5451825063683	26.83529255952205	18.41935071324511
20	25.16995409180092	30.57421431077853	27.302566753943978	16.95326484347657
21	23.267587864797694	28.155284130654724	31.847874418979334	16.729253585568248
22	22.755711672661388	23.06469428743985	31.882600260056954	22.29699377984181
23	19.402961299574777	28.227056937180816	31.456378108429277	20.913603654815127
24	19.064987386281974	27.706169321016503	34.612652130359805	18.616191162341714
25	20.145493537033996	29.581910886118813	30.354253642458552	19.918341934388643
26	18.00559891608043	33.72156793316618	27.202803918239987	21.0700292325134
27	18.000501544913075	32.925103688266624	30.90736392635937	18.167030840460928
28	15.959186441356794	30.111855438553693	33.66713529105762	20.261822829031896
29	17.201852712370364	28.565531485141847	33.50675014825613	20.725865654231658
30	20.195602516098816	29.391533175466535	31.348149995608072	19.064714312826577
31	23.2532515083895	28.238981144733028	28.325454405607836	20.182312941269636
32	24.14720297686476	28.35408160618165	27.848531615761985	19.650183801191602
33	23.244877255757412	27.90114376816792	28.21376736235164	20.640211613723032
34	17.84070806126494	28.2229608353499	30.820845153241994	23.115485950143157
35	18.517247547003908	28.77083721135567	29.923207193118913	22.78870804852151
36	23.26508469145658	27.730108760606058	28.918569950723892	20.086236597213468
37	18.518248816340353	31.519958711293544	29.93540447412652	20.026387998239585
38	19.06184704154494	30.247846019339974	27.787181112783433	22.903125826331657
39	18.529535852496643	29.405505433934202	29.087283833914906	22.977674879654252
40	21.821436357272926	27.58310421711888	28.916430875323307	21.679028550284883
41	17.942109337701297	26.468281835472336	30.066616269443397	25.522992557382974
42	19.547098571780317	27.10750128230743	29.480418585197327	23.864981560714924
43	19.877153354866692	27.398597585757578	29.273064808068046	23.45118425130768
44	19.35567407954903	29.226232710467954	28.91420077543759	22.50389243454543
45	18.346531125140007	31.390521893436723	26.226384266235698	24.036562715187575
46	20.15250242238911	30.214758618994715	27.627114555679906	22.00562440293627
47	18.5656725730938	28.05811549277698	30.296134508702167	23.080077425427053
48	19.447881882987115	27.5653089302757	30.724404711245302	22.262404475491884
49	19.83928716905204	26.989806623032564	30.408140137656332	22.76276607025907
50	18.2428087243328	28.69414908263248	30.127147552055767	22.935894640978947
51	17.491538136300974	30.337778210649773	27.90587704139475	24.264806611654503
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1073.0
1	2212.0
2	3351.0
3	13224.5
4	23098.0
5	15718.0
6	8338.0
7	7685.0
8	7032.0
9	7715.0
10	8398.0
11	8764.0
12	9130.0
13	9352.5
14	9575.0
15	9504.0
16	9433.0
17	9107.0
18	8781.0
19	9042.0
20	9303.0
21	10099.5
22	10896.0
23	11935.0
24	12974.0
25	16008.5
26	22386.0
27	25729.0
28	32419.0
29	39109.0
30	46551.0
31	53993.0
32	59769.0
33	65545.0
34	74290.5
35	83036.0
36	86703.5
37	90371.0
38	101859.0
39	113347.0
40	129520.5
41	145694.0
42	159478.5
43	173263.0
44	177300.5
45	181338.0
46	188791.5
47	196245.0
48	200124.0
49	204003.0
50	186930.0
51	169857.0
52	152854.5
53	135852.0
54	122916.5
55	109981.0
56	99083.5
57	88186.0
58	79156.5
59	70127.0
60	58104.0
61	46081.0
62	37487.5
63	28894.0
64	22489.5
65	16085.0
66	12456.0
67	8827.0
68	7254.5
69	5682.0
70	4202.0
71	2722.0
72	2118.5
73	1515.0
74	1144.0
75	483.0
76	193.0
77	168.5
78	144.0
79	112.0
80	80.0
81	66.5
82	53.0
83	39.0
84	25.0
85	19.0
86	13.0
87	10.5
88	8.0
89	6.5
90	5.0
91	4.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2197211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.423018399148745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01713094714707	19.054138245709698
2	9.116361249883767	4.452981170783957
3	3.347780640138425	2.452887245112442
4	1.6024646439436012	1.565480939320797
5	0.9483551732966604	1.1580847923176116
6	0.6374067762682522	0.9340438454724964
7	0.46175013633274364	0.7894132452824826
8	0.35053749761367065	0.684894700304819
9	0.2916868416092028	0.6411485789470031
>10	3.236961874496163	18.6382096039892
>50	1.2132948634645024	21.522151321518344
>100	0.7706496500665827	26.156973301717596
>500	0.003559146968229354	0.5517914602681003
>1k	0.0018732352464365019	0.6321285636783303
>5k	0.0	0.0
>10k+	1.873235246436502E-4	0.7656729855772263
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16736	0.7616928915793704	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2730	0.12424842220433086	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.102448513137791E-5	0.0	0.0	0.08920399542875036	0.0
2	9.102448513137791E-5	0.0	0.0	0.2964212358303322	0.0
3	9.102448513137791E-5	0.0	0.0	0.4484321259997333	0.0
4	9.102448513137791E-5	0.0	0.0	0.6561955133121034	0.0
5	9.102448513137791E-5	0.0	0.0	1.2095788706683155	0.0
6	9.102448513137791E-5	0.0	0.0	1.71226158980635	0.0
7	9.102448513137791E-5	0.0	0.0	2.0678032287295123	0.0
8	9.102448513137791E-5	0.0	0.0	2.777202553600906	0.0
9	9.102448513137791E-5	0.0	0.0	3.033390967003169	0.0
10	9.102448513137791E-5	0.0	0.0	3.485828170348683	0.0
11	9.102448513137791E-5	0.0	0.0	4.198458864442241	0.0
12	9.102448513137791E-5	0.0	0.0	4.723761168135423	0.0
13	9.102448513137791E-5	0.0	0.0	4.9374866592238975	0.0
14	9.102448513137791E-5	0.0	0.0	5.0400712539669605	0.0
15	9.102448513137791E-5	0.0	0.0	5.174377881778309	0.0
16	9.102448513137791E-5	0.0	0.0	5.445175725044158	0.0
17	9.102448513137791E-5	0.0	0.0	5.8122319613364395	0.0
18	9.102448513137791E-5	0.0	0.0	6.202499441337222	0.0
19	9.102448513137791E-5	0.0	0.0	6.497828383345978	0.0
20	9.102448513137791E-5	0.0	0.0	6.7897894194048725	0.0
21	9.102448513137791E-5	0.0	0.0	7.151657260044666	0.0
22	9.102448513137791E-5	0.0	0.0	7.582248586958649	0.0
23	9.102448513137791E-5	0.0	0.0	7.987398570278412	0.0
24	9.102448513137791E-5	0.0	0.0	8.287961420182222	0.0
25	9.102448513137791E-5	0.0	0.0	8.539826170540744	0.0
26	9.102448513137791E-5	0.0	0.0	8.786866623187304	0.0
27	9.102448513137791E-5	0.0	0.0	9.070453406614112	0.0
28	9.102448513137791E-5	0.0	0.0	9.355041459377365	0.0
29	9.102448513137791E-5	0.0	0.0	9.684049460884731	0.0
30	9.102448513137791E-5	0.0	0.0	10.104582582191696	0.0
31	9.102448513137791E-5	0.0	0.0	10.442556495484503	0.0
32	9.102448513137791E-5	0.0	0.0	10.742163588294433	0.0
33	9.102448513137791E-5	0.0	0.0	11.039039946550423	0.0
34	9.102448513137791E-5	0.0	0.0	11.360447403549317	0.0
35	9.102448513137791E-5	0.0	0.0	11.721040901397272	0.0
36	9.102448513137791E-5	0.0	0.0	12.038852891233477	0.0
37	9.102448513137791E-5	0.0	0.0	12.378556269743779	0.0
38	9.102448513137791E-5	0.0	0.0	12.773420486243698	0.0
39	9.102448513137791E-5	0.0	0.0	13.482273664204303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	55	1.8189894E-12	45.0	1
CGTATTA	20	7.0346927E-4	45.0	41
GCGCCTA	25	3.891971E-5	45.0	20
GAGCGTA	35	1.2126111E-7	45.0	9
ACTACGC	20	7.0346927E-4	45.0	21
CGTCGAG	25	3.891971E-5	45.0	37
TCGACTA	20	7.0346927E-4	45.0	30
TCGACCG	25	3.891971E-5	45.0	13
TACGCGG	125	0.0	44.999996	2
TACGTAG	175	0.0	43.714287	1
CGGTCTA	570	0.0	43.421055	31
TCTAGCG	105	0.0	42.857143	1
CGACCCG	100	0.0	42.75	32
ACGTAGG	385	0.0	42.662334	2
CGTTTTT	5985	0.0	42.5188	1
CGGGTAT	150	0.0	42.0	6
CTATGCG	70	0.0	41.785713	1
CGTTAGG	330	0.0	41.59091	2
TAGTACG	65	0.0	41.53846	1
TCACGAC	605	0.0	41.280994	25
>>END_MODULE