FastQCFastQC Report
Thu 26 May 2016
SRR1548111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548111_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1716612
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66650.38826479134481173No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTA207.0342596E-445.00000430
CCCGTAA207.0342596E-445.00000429
ACCGTTA207.0342596E-445.00000442
TACCGTA207.0342596E-445.00000445
AGTCGAC207.0342596E-445.00000428
ACGGTCG207.0342596E-445.00000431
GTCGATC351.2123928E-745.044
CGTTCGG351.2123928E-745.02
TAGTGCG1650.045.01
TAGTCCG453.8562575E-1045.01
TATATCG302.1661308E-644.99999640
CCTGTCG302.1661308E-644.9999969
CTACGCG302.1661308E-644.9999961
CGGTCTA4400.043.97727231
TATCGCG1750.043.7142871
CTAGCGG1650.043.6363642
ACGTTAG1000.042.751
AATGCGG3300.042.2727282
TTCGCGG1600.042.1875042
GCGAACG750.042.01