##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548111_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1716612
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.517032387050772	31.0	31.0	33.0	30.0	34.0
2	31.912586536736317	31.0	31.0	34.0	30.0	34.0
3	32.01679354449345	33.0	31.0	34.0	30.0	34.0
4	35.72319545709805	37.0	35.0	37.0	35.0	37.0
5	35.61658779036847	37.0	35.0	37.0	33.0	37.0
6	35.666533846903086	37.0	35.0	37.0	33.0	37.0
7	36.08650819171717	37.0	35.0	37.0	35.0	37.0
8	36.124799896540395	37.0	35.0	37.0	35.0	37.0
9	37.95288451904099	39.0	38.0	39.0	35.0	39.0
10	37.32635621794558	39.0	37.0	39.0	34.0	39.0
11	36.90957304271437	39.0	37.0	39.0	33.0	39.0
12	36.31470943929088	38.0	35.0	39.0	32.0	39.0
13	36.07029544241797	38.0	35.0	39.0	32.0	39.0
14	37.128088933317485	39.0	35.0	41.0	32.0	41.0
15	37.36042390476124	39.0	35.0	41.0	32.0	41.0
16	37.48678559860935	39.0	35.0	41.0	33.0	41.0
17	37.42062213243295	39.0	35.0	41.0	32.0	41.0
18	37.35258812125279	39.0	35.0	41.0	32.0	41.0
19	37.30977879683935	39.0	35.0	41.0	32.0	41.0
20	37.20920044832496	39.0	35.0	41.0	32.0	41.0
21	37.075342593434044	39.0	35.0	41.0	32.0	41.0
22	36.974474138593926	39.0	35.0	41.0	32.0	41.0
23	36.894586546057	39.0	35.0	41.0	32.0	41.0
24	36.92246413283841	39.0	35.0	41.0	32.0	41.0
25	36.91597868359303	39.0	35.0	41.0	32.0	41.0
26	36.78888473341675	38.0	35.0	41.0	31.0	41.0
27	36.73107667894667	38.0	35.0	41.0	31.0	41.0
28	36.71825083361878	38.0	35.0	41.0	31.0	41.0
29	36.7059277227469	38.0	35.0	41.0	31.0	41.0
30	36.642802799933825	38.0	35.0	41.0	31.0	41.0
31	36.564699536062896	38.0	35.0	41.0	31.0	41.0
32	36.46549249335319	38.0	35.0	41.0	30.0	41.0
33	36.37065044401414	38.0	35.0	41.0	30.0	41.0
34	36.316720959657744	38.0	35.0	41.0	30.0	41.0
35	36.24457128343504	38.0	35.0	41.0	30.0	41.0
36	36.10797605982016	38.0	35.0	41.0	30.0	41.0
37	36.02926345615666	38.0	35.0	40.0	30.0	41.0
38	35.97182648146465	38.0	35.0	40.0	30.0	41.0
39	35.94074374407263	38.0	35.0	40.0	30.0	41.0
40	35.81443273145009	38.0	35.0	40.0	29.0	41.0
41	35.7595933152046	38.0	35.0	40.0	29.0	41.0
42	35.71881473507118	38.0	35.0	40.0	29.0	41.0
43	35.60407651816485	38.0	34.0	40.0	29.0	41.0
44	35.43142189382341	38.0	34.0	40.0	28.0	41.0
45	35.4120605005674	38.0	34.0	40.0	28.0	41.0
46	35.33846437051588	38.0	34.0	40.0	27.0	41.0
47	35.248034500516134	37.0	34.0	40.0	27.0	41.0
48	35.16484971560259	37.0	34.0	40.0	27.0	41.0
49	35.0740674071951	37.0	34.0	40.0	27.0	41.0
50	34.892033260865006	37.0	34.0	40.0	26.0	41.0
51	34.51655936227872	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	11.0
15	30.0
16	81.0
17	199.0
18	491.0
19	986.0
20	1731.0
21	2942.0
22	4511.0
23	6738.0
24	9966.0
25	14294.0
26	19281.0
27	23580.0
28	27747.0
29	32696.0
30	39707.0
31	48842.0
32	61444.0
33	79280.0
34	135375.0
35	215343.0
36	114530.0
37	150358.0
38	242421.0
39	483914.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.113779933962945	24.427768185239294	31.26361693848115	12.194834942316609
2	28.111361216163	25.9489622582156	32.36427334773379	13.575403177887607
3	28.969446794033832	26.301866700221133	31.501061393023	13.227625112722036
4	25.548114541899974	27.897043711683246	31.477759680114087	15.077082066302694
5	23.990628051068033	32.68216696609368	29.32409886450753	14.003106118330757
6	22.77014258318129	40.975246590376855	25.8861058876438	10.36850493879805
7	91.66084123843945	2.924772750044856	4.168152150864611	1.2462338606510965
8	92.7757699468488	1.9979471190927245	3.8476370898024714	1.3786458442560112
9	88.0595032540842	5.19034004189648	5.029616477107232	1.7205402269120804
10	45.31967619939742	37.64246084729688	9.395367153439448	7.642495799866249
11	35.56831712699201	23.11920224255685	26.312235962465603	15.000244667985543
12	34.38732806248587	22.694528524791856	26.006692251947438	16.911451160774828
13	23.83840961149054	32.00822317448556	26.57245784137592	17.580909372647984
14	16.244497883039383	35.63595034871013	28.47079013778303	19.648761630467458
15	14.815753356029202	29.374838344366694	39.34202953259094	16.467378767013162
16	20.085552238945084	25.65943847532232	38.01068616554003	16.244323120192565
17	19.91632354894408	25.613301083762668	27.407999012007373	27.062376355285878
18	21.418876251593254	28.74004143044555	31.003686330982188	18.837395986979004
19	26.033372713228147	28.215286855736764	27.320326317187575	18.43101411384751
20	29.06760525966264	27.5277698163592	26.665664693011582	16.73896023096658
21	24.613948871381535	28.096215102772206	29.49606550577533	17.793770520070932
22	23.68549212052578	23.826292720777907	29.015642439875755	23.47257271882056
23	20.73625257192656	28.188023851633332	29.17788061600408	21.89784296043602
24	20.180390210484372	25.73627587363947	35.5378501373636	18.54548377851256
25	18.622029905418348	28.687088287860043	32.52383182687759	20.167049979844016
26	18.629369944984656	35.79480977646667	26.10951106015803	19.46630921839064
27	18.389420556305094	34.71500840026751	29.37711026137531	17.518460782052088
28	15.754171589153518	29.98907149664572	36.51972606506304	17.73703084913772
29	17.590696092069727	26.513213236305	35.820208643537384	20.075882028087886
30	19.282400449257025	29.453423371152017	32.34906898006072	18.915107199530237
31	25.740470181963076	27.51938119971199	27.19927391862576	19.540874699699177
32	25.974011599592682	28.640135336348578	27.82638126728696	17.55947179677178
33	24.149370970260023	28.93589232744499	27.600762432046377	19.313974270248607
34	19.321430818379458	27.45215575796977	30.150552367104506	23.07586105654627
35	19.15121180557983	27.673289013475383	31.386358711228862	21.789140469715928
36	25.205928887832545	26.320857596241897	28.66291276071704	19.810300755208516
37	19.85399146691273	31.581627065405577	30.203913289665923	18.360468178015765
38	19.14818258290167	31.489294028004	27.23061472248825	22.13190866660608
39	20.121961165365263	29.603136876591797	28.882822676294932	21.392079281748003
40	21.927902170088522	27.03173460281065	28.76549855179854	22.27486467530228
41	17.297793560804656	26.272098761979993	30.093987459018113	26.33612021819724
42	21.002649404757744	26.90695393018341	27.366288945900415	24.724107719158436
43	21.612804757277708	26.663218013156147	27.726125647496346	23.9978515820698
44	19.10926872234378	29.946778887716036	29.92883656877617	21.015115821164013
45	18.871824267801927	33.09885984718737	26.398568808793133	21.630747076217574
46	20.660347242125766	30.826185532898524	27.775467024580973	20.738000200394733
47	19.74633755327354	28.299056513644317	28.980049073407386	22.974556859674756
48	21.39714740430569	25.31212644441493	31.8843163160924	21.406409835186984
49	20.025026039664176	25.66153562948412	31.530887585546413	22.78255074530529
50	18.60548568925302	30.314538171701	29.431403252453087	21.64857288659289
51	17.820159709940278	30.644315663644434	28.208937138969087	23.3265874874462
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	693.0
1	1704.5
2	2716.0
3	6699.0
4	10682.0
5	7669.5
6	4657.0
7	4435.0
8	4213.0
9	4310.5
10	4408.0
11	4452.0
12	4496.0
13	4656.5
14	4817.0
15	4460.5
16	4104.0
17	4378.0
18	4652.0
19	4560.5
20	4469.0
21	5640.0
22	6811.0
23	8513.5
24	10216.0
25	12718.0
26	19358.5
27	23497.0
28	27876.0
29	32255.0
30	38703.0
31	45151.0
32	50439.0
33	55727.0
34	58114.5
35	60502.0
36	67358.0
37	74214.0
38	82003.5
39	89793.0
40	107945.5
41	126098.0
42	142152.0
43	158206.0
44	160380.5
45	162555.0
46	158601.0
47	154647.0
48	146671.0
49	138695.0
50	130490.0
51	122285.0
52	109679.5
53	97074.0
54	85746.0
55	74418.0
56	67292.5
57	60167.0
58	56174.0
59	52181.0
60	46585.0
61	40989.0
62	35346.0
63	29703.0
64	22945.0
65	16187.0
66	12722.5
67	9258.0
68	7049.5
69	4841.0
70	3684.5
71	2528.0
72	2059.5
73	1591.0
74	1306.0
75	805.0
76	589.0
77	363.0
78	137.0
79	95.5
80	54.0
81	38.5
82	23.0
83	26.5
84	30.0
85	24.5
86	19.0
87	15.0
88	11.0
89	8.0
90	5.0
91	3.0
92	1.0
93	2.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1716612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.280421555971913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7923383547914	16.18222258771916
2	8.511622692529379	3.4523859265974504
3	2.7159159642350432	1.6523976199584185
4	1.2519806191088134	1.0156277894173376
5	0.7019689844381414	0.7118113461811497
6	0.44986868710840566	0.5474115971634055
7	0.33720174740482173	0.47870155107461004
8	0.263156692985131	0.4269542935211147
9	0.19592588039594885	0.3576113503339298
>10	2.9913743465591747	15.27868768207413
>50	1.5343610784875352	22.868456554043718
>100	1.249962812218247	36.02057786712153
>500	0.0034577117903414256	0.4707647979751249
>1k	5.762852983902376E-4	0.1469094714634389
>5k	2.881426491951188E-4	0.3894795653555371
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6665	0.38826479134481173	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1650856454457967E-4	0.0	0.0	0.05219583691597169	0.0
2	1.1650856454457967E-4	0.0	0.0	0.1907245201594769	0.0
3	1.1650856454457967E-4	0.0	0.0	0.2816594547865214	0.0
4	1.1650856454457967E-4	0.0	0.0	0.4239164120954531	0.0
5	1.1650856454457967E-4	0.0	0.0	0.7596940951129317	0.0
6	1.1650856454457967E-4	0.0	0.0	1.0090806775206045	0.0
7	1.1650856454457967E-4	0.0	0.0	1.1855328985233704	0.0
8	1.1650856454457967E-4	0.0	0.0	1.5902836517512402	0.0
9	1.1650856454457967E-4	0.0	0.0	1.7059766563440077	0.0
10	1.1650856454457967E-4	0.0	0.0	1.974761914748353	0.0
11	1.1650856454457967E-4	0.0	0.0	2.4541946578492984	0.0
12	1.1650856454457967E-4	0.0	0.0	2.7978366689735363	0.0
13	1.1650856454457967E-4	0.0	0.0	2.9325788238693424	0.0
14	1.1650856454457967E-4	0.0	0.0	2.9807551153085265	0.0
15	1.1650856454457967E-4	0.0	0.0	3.067670504458783	0.0
16	1.1650856454457967E-4	0.0	0.0	3.272550815210426	0.0
17	1.1650856454457967E-4	0.0	0.0	3.5501324702378874	0.0
18	1.1650856454457967E-4	0.0	0.0	3.8448408842534016	0.0
19	1.1650856454457967E-4	0.0	0.0	4.046051175221891	0.0
20	1.1650856454457967E-4	0.0	0.0	4.2516887916430735	0.0
21	1.1650856454457967E-4	0.0	0.0	4.521580881410593	0.0
22	1.1650856454457967E-4	0.0	0.0	4.837202582761859	0.0
23	1.1650856454457967E-4	0.0	0.0	5.151251418491773	0.0
24	1.1650856454457967E-4	0.0	0.0	5.378792645047337	0.0
25	1.1650856454457967E-4	0.0	0.0	5.5684685881259135	0.0
26	1.1650856454457967E-4	0.0	0.0	5.7554065799376914	0.0
27	1.1650856454457967E-4	0.0	0.0	5.932266580916363	0.0
28	1.1650856454457967E-4	0.0	0.0	6.124330949568103	0.0
29	1.1650856454457967E-4	0.0	0.0	6.35297900748684	0.0
30	1.1650856454457967E-4	0.0	0.0	6.629803356844762	0.0
31	1.1650856454457967E-4	0.0	0.0	6.884374570374669	0.0
32	1.1650856454457967E-4	0.0	0.0	7.113372153987039	0.0
33	1.7476284681686952E-4	0.0	0.0	7.337068597912633	0.0
34	1.7476284681686952E-4	0.0	0.0	7.570959541235876	0.0
35	1.7476284681686952E-4	0.0	0.0	7.8471430934888025	0.0
36	1.7476284681686952E-4	0.0	0.0	8.099500644292362	0.0
37	1.7476284681686952E-4	0.0	0.0	8.369975276882604	0.0
38	1.7476284681686952E-4	0.0	0.0	8.617963756515742	0.0
39	1.7476284681686952E-4	0.0	0.0	8.880108026741047	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTA	20	7.0342596E-4	45.000004	30
CCCGTAA	20	7.0342596E-4	45.000004	29
ACCGTTA	20	7.0342596E-4	45.000004	42
TACCGTA	20	7.0342596E-4	45.000004	45
AGTCGAC	20	7.0342596E-4	45.000004	28
ACGGTCG	20	7.0342596E-4	45.000004	31
GTCGATC	35	1.2123928E-7	45.0	44
CGTTCGG	35	1.2123928E-7	45.0	2
TAGTGCG	165	0.0	45.0	1
TAGTCCG	45	3.8562575E-10	45.0	1
TATATCG	30	2.1661308E-6	44.999996	40
CCTGTCG	30	2.1661308E-6	44.999996	9
CTACGCG	30	2.1661308E-6	44.999996	1
CGGTCTA	440	0.0	43.977272	31
TATCGCG	175	0.0	43.714287	1
CTAGCGG	165	0.0	43.636364	2
ACGTTAG	100	0.0	42.75	1
AATGCGG	330	0.0	42.272728	2
TTCGCGG	160	0.0	42.187504	2
GCGAACG	75	0.0	42.0	1
>>END_MODULE