FastQCFastQC Report
Thu 26 May 2016
SRR1548110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548110_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1450067
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51960.3583282703488873No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20790.14337268553797858No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20130.1388211717113761No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15850.10930529416916597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA351.2122291E-745.00000434
TCCTCGA302.1658961E-645.00000439
GATCACG351.2122291E-745.0000041
GGTGCGT302.1658961E-645.0000048
TCCGTAC351.2122291E-745.00000421
CGAGCCT351.2122291E-745.00000435
ATACGAC351.2122291E-745.00000410
AGGGCGT302.1658961E-645.0000046
TCGACAG600.045.0000041
CGATAAG302.1658961E-645.00000410
CACCCGT302.1658961E-645.00000425
CGAAGCA302.1658961E-645.00000428
ACGCGAG207.0338993E-445.01
TCCGCGA253.8913127E-545.037
CGTATCT253.8913127E-545.036
GATCGAC207.0338993E-445.011
CGACGTG207.0338993E-445.015
TTTACGC207.0338993E-445.021
CGAAACG253.8913127E-545.037
CCGATAT207.0338993E-445.028