##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548110_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1450067
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62175402929658	31.0	31.0	33.0	30.0	34.0
2	32.044076584047495	33.0	31.0	34.0	30.0	34.0
3	32.12882025451238	33.0	31.0	34.0	30.0	34.0
4	35.81316242628789	37.0	35.0	37.0	35.0	37.0
5	35.71751581133837	37.0	35.0	37.0	33.0	37.0
6	35.77821024821612	37.0	35.0	37.0	35.0	37.0
7	36.116581509681964	37.0	35.0	37.0	35.0	37.0
8	36.1426134102769	37.0	35.0	37.0	35.0	37.0
9	37.97572456996814	39.0	38.0	39.0	35.0	39.0
10	37.45941946130765	39.0	37.0	39.0	35.0	39.0
11	37.07628475098047	39.0	37.0	39.0	33.0	39.0
12	36.19198768056924	38.0	35.0	39.0	32.0	39.0
13	35.815763685402125	38.0	35.0	39.0	31.0	39.0
14	36.82792588204545	39.0	35.0	41.0	31.0	41.0
15	37.11687873732731	39.0	35.0	41.0	32.0	41.0
16	37.30484522439308	39.0	35.0	41.0	33.0	41.0
17	37.258356338017485	39.0	35.0	41.0	33.0	41.0
18	37.21556659106096	38.0	35.0	41.0	33.0	41.0
19	37.14875174733305	38.0	35.0	41.0	32.0	41.0
20	36.99234793978485	38.0	35.0	41.0	32.0	41.0
21	36.83124090128249	38.0	35.0	41.0	32.0	41.0
22	36.726478155836936	38.0	35.0	41.0	32.0	41.0
23	36.68401460070466	38.0	35.0	41.0	32.0	41.0
24	36.71921918090681	38.0	35.0	41.0	32.0	41.0
25	36.72802842903121	38.0	35.0	41.0	32.0	41.0
26	36.61551293836767	38.0	35.0	41.0	32.0	41.0
27	36.52807766813533	38.0	35.0	40.0	31.0	41.0
28	36.52725080979017	38.0	35.0	40.0	31.0	41.0
29	36.52004355660807	38.0	35.0	40.0	31.0	41.0
30	36.481477062783995	38.0	35.0	40.0	31.0	41.0
31	36.34697844996128	38.0	35.0	40.0	31.0	41.0
32	36.20280373251719	38.0	35.0	40.0	31.0	41.0
33	36.09628451650855	38.0	35.0	40.0	30.0	41.0
34	36.007438966613265	38.0	35.0	40.0	30.0	41.0
35	35.934885077724	38.0	35.0	40.0	30.0	41.0
36	35.7551595891776	38.0	35.0	40.0	30.0	41.0
37	35.69366381001706	38.0	35.0	40.0	30.0	41.0
38	35.65071544969991	38.0	35.0	40.0	29.0	41.0
39	35.642267564188415	37.0	35.0	40.0	29.0	41.0
40	35.51168877024303	37.0	35.0	40.0	29.0	41.0
41	35.459735308782285	37.0	34.0	40.0	29.0	41.0
42	35.4102589742405	37.0	34.0	40.0	29.0	41.0
43	35.30350321743754	37.0	34.0	40.0	28.0	41.0
44	35.172882356470424	37.0	34.0	40.0	28.0	41.0
45	35.14786627100679	37.0	34.0	40.0	28.0	41.0
46	35.07859567868243	36.0	34.0	40.0	27.0	41.0
47	35.019787361549504	36.0	34.0	40.0	27.0	41.0
48	34.94919131322897	36.0	34.0	40.0	27.0	41.0
49	34.88737141111411	36.0	34.0	40.0	27.0	41.0
50	34.742929119826876	36.0	34.0	40.0	27.0	41.0
51	34.36046334410755	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	5.0
13	8.0
14	14.0
15	35.0
16	76.0
17	218.0
18	469.0
19	949.0
20	1629.0
21	2611.0
22	4127.0
23	5825.0
24	9130.0
25	13237.0
26	17644.0
27	20514.0
28	23345.0
29	26654.0
30	32457.0
31	39980.0
32	51051.0
33	68749.0
34	130228.0
35	227359.0
36	87638.0
37	114666.0
38	184773.0
39	386550.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.7716353796066	24.807543375581957	30.873401022159662	12.54742022265178
2	29.548082950649867	27.43452543916936	29.697386396628573	13.320005213552202
3	29.4987059218643	25.1936634652054	31.25159044375191	14.056040169178392
4	27.039026472569887	27.452110833499415	30.30639273909412	15.20246995483657
5	24.275430031853702	32.92365111405197	28.68653655313858	14.11438230095575
6	23.29761314477193	40.867215101095326	25.08711666426448	10.748055089868261
7	89.35738831378136	3.9227152952242896	5.200794170200411	1.5191022207939358
8	90.27231155525917	3.269159287122595	4.961701769642368	1.4968273879758658
9	85.0291055516745	6.471425113460275	6.258607360901255	2.2408619739639617
10	48.70781832839448	32.23375195766816	11.75980144365743	7.298628270279925
11	43.87411064454263	19.553165474422908	23.230512796994898	13.342211084039565
12	42.61899622569164	19.84777255119936	23.713249111937586	13.819982111171413
13	23.16141254162739	37.71480903985816	22.325864942792297	16.797913475722158
14	15.060959252227658	40.2110385244268	25.80273877000166	18.92526345334388
15	14.167966031914386	28.827219707779015	42.20191204958116	14.802902210725435
16	17.746214485261717	23.41057344246852	42.43238415880094	16.410827913468825
17	17.901172842358317	24.606311294581563	26.712972572991454	30.779543290068666
18	21.025925008982345	28.223316577785717	31.167732249613294	19.583026163618648
19	28.48027022199664	28.02311893174591	24.230949328548267	19.26566151770918
20	32.4638102929037	25.345380592758815	25.939697958783974	16.251111155553502
21	24.135712349843143	30.363769398241597	27.18908850418636	18.311429747728898
22	23.336507899290172	25.932387951729126	26.006591419568885	24.724512729411813
23	20.669044947578286	30.576587150800616	26.280371872472095	22.473996029149
24	19.781361826729384	25.989488761553776	37.05746010356763	17.17168930814921
25	17.345336456867166	29.557806639279427	33.581689673649564	19.515167230203843
26	16.693435544702417	37.867491640041465	25.93818078750844	19.500892027747682
27	18.25288072895942	37.10842326595943	28.047462634485164	16.59123337059598
28	14.89917362439115	31.892595307665093	36.62637657432381	16.58185449361995
29	15.878024946433507	26.791175856012174	36.61175656021412	20.719042637340205
30	19.039465072993178	32.755107177806266	30.391906029169686	17.813521720030867
31	28.785980234016773	28.495579859413393	23.53574007270009	19.182699833869744
32	28.754809260537616	29.429053967851143	24.849610397312677	16.966526374298567
33	26.154032882618527	29.87020599737805	24.69858289306632	19.2771782269371
34	19.162355946311447	28.84880491729003	28.147802825662538	23.841036310735987
35	19.85108274307325	26.947444497392187	30.719201250700827	22.48227150883373
36	27.41831929145343	27.29598011678081	27.965397460944907	17.320303130820854
37	20.288441844411327	33.447902752079735	28.951765677034235	17.311889726474707
38	19.077187467889413	33.3390112318948	25.945490794563288	21.6383105056525
39	19.843014150380636	33.26735937029117	27.533141572079085	19.356484907249115
40	24.539900570111588	28.2830379561772	25.64867692320424	21.528384550506978
41	17.54801674681239	25.708674150918544	29.5812538317195	27.16205527054957
42	20.438710763019916	26.325680123746004	26.035693523126863	27.199915590107217
43	21.618587279070553	27.493695118915195	25.414204998803502	25.473512603210747
44	19.413723641735174	31.122975696985034	28.405239206188405	21.058061455091384
45	17.933378250798064	37.45564860106464	23.24444318779753	21.366529960339765
46	20.96468645931533	35.493187556161196	24.486385801483657	19.05574018303982
47	20.786763646093593	28.51771676757005	27.44183544622421	23.253684140112146
48	21.767407988734313	24.838024725754053	32.28450823306785	21.110059052443784
49	19.36303632866619	26.102173209927543	31.749912245434174	22.78487821597209
50	17.941308918829268	32.20520155275584	29.155480401940046	20.698009126474844
51	17.646012218745753	33.72775189008508	25.445100122959836	23.181135768209334
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	580.0
1	1646.0
2	2712.0
3	5987.5
4	9263.0
5	7122.5
6	4982.0
7	4866.0
8	4750.0
9	4788.5
10	4827.0
11	4892.5
12	4958.0
13	4768.5
14	4579.0
15	4329.0
16	4079.0
17	4164.5
18	4250.0
19	3857.0
20	3464.0
21	4283.0
22	5102.0
23	5087.0
24	5072.0
25	8297.5
26	13294.5
27	15066.0
28	20588.0
29	26110.0
30	30519.5
31	34929.0
32	37231.0
33	39533.0
34	46464.0
35	53395.0
36	55231.0
37	57067.0
38	64505.5
39	71944.0
40	88199.5
41	104455.0
42	126260.5
43	148066.0
44	150796.0
45	153526.0
46	143051.0
47	132576.0
48	122312.0
49	112048.0
50	101866.0
51	91684.0
52	83883.5
53	76083.0
54	68349.5
55	60616.0
56	55439.5
57	50263.0
58	47504.5
59	44746.0
60	42881.0
61	41016.0
62	34271.0
63	27526.0
64	21727.5
65	15929.0
66	12857.5
67	9786.0
68	7878.0
69	5970.0
70	4803.5
71	3637.0
72	2900.0
73	2163.0
74	1481.5
75	605.0
76	410.0
77	299.5
78	189.0
79	183.0
80	177.0
81	163.5
82	150.0
83	89.5
84	29.0
85	23.0
86	17.0
87	15.0
88	13.0
89	8.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1450067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.590612689978464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21585539237152	13.474208011001352
2	8.39134861387733	2.784352295988529
3	2.4404402125032165	1.214651950760688
4	1.1278583208380573	0.748474422807747
5	0.6010949814911473	0.49862670139046994
6	0.3960148170058151	0.39420770690617046
7	0.28771741650794763	0.3341385755011203
8	0.1940140689872476	0.25750498199793503
9	0.16255857393063364	0.24272517055665357
>10	1.9288254096407194	8.140277086297122
>50	1.217749154359844	15.005301437681007
>100	2.026111366510621	55.13809673104612
>500	0.007496403822649781	0.7640459513113327
>1k	0.002498801274216594	0.6443744385901148
>5k	4.16466879036099E-4	0.35901453816365386
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5196	0.3583282703488873	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2079	0.14337268553797858	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2013	0.1388211717113761	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1585	0.10930529416916597	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03730862091199924	0.0
2	0.0	0.0	0.0	0.13620060314454435	0.0
3	0.0	0.0	0.0	0.19854255010285732	0.0
4	0.0	0.0	0.0	0.30591689901225255	0.0
5	0.0	0.0	0.0	0.5228723914136382	0.0
6	0.0	0.0	0.0	0.6967953894544183	0.0
7	0.0	0.0	0.0	0.8056869096393477	0.0
8	0.0	0.0	0.0	1.0520203549215312	0.0
9	0.0	0.0	0.0	1.1266375967455298	0.0
10	0.0	0.0	0.0	1.2747686831022291	0.0
11	0.0	0.0	0.0	1.5421356392497727	0.0
12	0.0	0.0	0.0	1.7398506413841568	0.0
13	0.0	0.0	0.0	1.8223295889086504	0.0
14	0.0	0.0	0.0	1.8553625453168716	0.0
15	0.0	0.0	0.0	1.9146011873934101	0.0
16	0.0	0.0	0.0	2.0479743349790045	0.0
17	6.89623307060984E-5	0.0	0.0	2.2096910004848054	0.0
18	6.89623307060984E-5	0.0	0.0	2.396096180383389	0.0
19	6.89623307060984E-5	0.0	0.0	2.5085047794343294	0.0
20	6.89623307060984E-5	0.0	0.0	2.6318783890675395	0.0
21	6.89623307060984E-5	0.0	0.0	2.7929054312662793	0.0
22	6.89623307060984E-5	0.0	0.0	2.9573805900003243	0.0
23	6.89623307060984E-5	0.0	0.0	3.1342689682614666	0.0
24	6.89623307060984E-5	0.0	0.0	3.267159379532118	0.0
25	6.89623307060984E-5	0.0	0.0	3.3816368485042414	0.0
26	6.89623307060984E-5	0.0	0.0	3.4854941185476256	0.0
27	6.89623307060984E-5	0.0	0.0	3.594109789409731	0.0
28	6.89623307060984E-5	0.0	0.0	3.7174833990429406	0.0
29	6.89623307060984E-5	0.0	0.0	3.847960128738879	0.0
30	6.89623307060984E-5	0.0	0.0	4.018848784228591	0.0
31	1.379246614121968E-4	0.0	0.0	4.178634504474621	0.0
32	1.379246614121968E-4	0.0	0.0	4.318696998138707	0.0
33	1.379246614121968E-4	0.0	0.0	4.452001183393595	0.0
34	1.379246614121968E-4	0.0	0.0	4.602821800647832	0.0
35	1.379246614121968E-4	0.0	0.0	4.79198547377466	0.0
36	1.379246614121968E-4	0.0	0.0	4.9586674270913	0.0
37	1.379246614121968E-4	0.0	0.0	5.114867106140613	0.0
38	1.379246614121968E-4	0.0	0.0	5.2699633878986285	0.0
39	1.379246614121968E-4	0.0	0.0	5.441472704364695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	35	1.2122291E-7	45.000004	34
TCCTCGA	30	2.1658961E-6	45.000004	39
GATCACG	35	1.2122291E-7	45.000004	1
GGTGCGT	30	2.1658961E-6	45.000004	8
TCCGTAC	35	1.2122291E-7	45.000004	21
CGAGCCT	35	1.2122291E-7	45.000004	35
ATACGAC	35	1.2122291E-7	45.000004	10
AGGGCGT	30	2.1658961E-6	45.000004	6
TCGACAG	60	0.0	45.000004	1
CGATAAG	30	2.1658961E-6	45.000004	10
CACCCGT	30	2.1658961E-6	45.000004	25
CGAAGCA	30	2.1658961E-6	45.000004	28
ACGCGAG	20	7.0338993E-4	45.0	1
TCCGCGA	25	3.8913127E-5	45.0	37
CGTATCT	25	3.8913127E-5	45.0	36
GATCGAC	20	7.0338993E-4	45.0	11
CGACGTG	20	7.0338993E-4	45.0	15
TTTACGC	20	7.0338993E-4	45.0	21
CGAAACG	25	3.8913127E-5	45.0	37
CCGATAT	20	7.0338993E-4	45.0	28
>>END_MODULE