FastQCFastQC Report
Thu 26 May 2016
SRR1548109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548109_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2315969
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101460.4380887654368431No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA207.0347707E-445.00000428
CGTACCG207.0347707E-445.00000430
CGGACCG207.0347707E-445.00000441
CTATCGT253.892037E-545.044
CCGCGTA253.892037E-545.040
TACGGGA9450.042.8571434
TAATGCG850.042.352941
CGTTTTT48550.042.1266751
AATCGCG750.042.01
CGACGGT6350.040.7480328
CCGCTCG1050.040.71428719
TCACGAC6650.040.60150525
CGCTAAT501.0822987E-940.511
CGGTCTA6450.040.11627631
CGTAAGG4100.040.0609742
CGAACGG900.040.02
AGTACGG1700.039.7058832
TAGCGCG1250.039.6000021
CACGACG6650.039.58646826
CGAGGGA19150.039.4778064
Produced by FastQC (version 0.11.5)