FastQCFastQC Report
Thu 26 May 2016
SRR1548108_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548108_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1704720
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48360.28368295086583134No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG21750.127586935097846No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19750.11585480313482566No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA207.0342456E-445.00000418
GTACGTC207.0342456E-445.00000425
GTACGAA253.8916012E-545.025
TATAGCG1750.043.7142871
CGTAAGG2750.043.3636322
TAACGGG3900.041.5384643
ATAACGG1300.041.5384642
ATAGCGG3600.040.02
CGTTAAC451.9292202E-840.037
TATCGCG451.9292202E-840.01
CGGTCTA4450.039.438231
TAGGGAC17250.039.3913045
CTAGACG800.039.3750041
CTATCGG403.459536E-739.37500425
TCACGAC4400.039.37525
CTCACGA4400.039.37524
CACGACG4400.039.37526
CGGGATC6050.039.0495876
ACGTAAG750.039.01
TCGTAAG1100.038.8636361
Produced by FastQC (version 0.11.5)