##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548108_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1704720
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.534460204608383	31.0	31.0	33.0	30.0	34.0
2	31.947015932235207	31.0	31.0	34.0	30.0	34.0
3	32.06642439814163	33.0	31.0	34.0	30.0	34.0
4	35.77264184147543	37.0	35.0	37.0	35.0	37.0
5	35.6559640292834	37.0	35.0	37.0	33.0	37.0
6	35.7066937678915	37.0	35.0	37.0	33.0	37.0
7	36.05941503590032	37.0	35.0	37.0	35.0	37.0
8	36.08234490121545	37.0	35.0	37.0	35.0	37.0
9	37.87439110235112	39.0	38.0	39.0	35.0	39.0
10	37.34085362992163	39.0	37.0	39.0	34.0	39.0
11	37.01064925618283	39.0	37.0	39.0	33.0	39.0
12	36.475620043174246	39.0	35.0	39.0	32.0	39.0
13	36.27282955558684	39.0	35.0	39.0	32.0	39.0
14	37.269549251489984	39.0	35.0	41.0	32.0	41.0
15	37.47551093434699	40.0	35.0	41.0	32.0	41.0
16	37.59726875967901	40.0	35.0	41.0	33.0	41.0
17	37.54125369562157	39.0	35.0	41.0	33.0	41.0
18	37.45316239617063	39.0	35.0	41.0	32.0	41.0
19	37.41413369937585	39.0	35.0	41.0	32.0	41.0
20	37.32018689286217	39.0	35.0	41.0	32.0	41.0
21	37.19289971373598	39.0	35.0	41.0	32.0	41.0
22	37.1081550049275	39.0	35.0	41.0	32.0	41.0
23	37.01502592801164	39.0	35.0	41.0	32.0	41.0
24	37.05477263128255	39.0	35.0	41.0	32.0	41.0
25	37.036153151250645	39.0	35.0	41.0	32.0	41.0
26	36.906926650710965	39.0	35.0	41.0	31.0	41.0
27	36.86539490356188	39.0	35.0	41.0	31.0	41.0
28	36.809163968276316	39.0	35.0	41.0	31.0	41.0
29	36.75495917218077	39.0	35.0	41.0	31.0	41.0
30	36.657952625651134	38.0	35.0	41.0	31.0	41.0
31	36.606232695105355	39.0	35.0	41.0	31.0	41.0
32	36.51292177014407	39.0	35.0	41.0	30.0	41.0
33	36.42194260641044	39.0	35.0	41.0	30.0	41.0
34	36.327420925430566	38.0	35.0	41.0	30.0	41.0
35	36.2480331080764	38.0	35.0	41.0	30.0	41.0
36	36.112793889905674	38.0	35.0	41.0	30.0	41.0
37	36.070291308836644	38.0	35.0	41.0	30.0	41.0
38	35.952501290534514	38.0	35.0	41.0	29.0	41.0
39	35.878951382045145	38.0	35.0	40.0	29.0	41.0
40	35.752411539724996	38.0	35.0	40.0	28.0	41.0
41	35.68582758458867	38.0	35.0	40.0	28.0	41.0
42	35.654545027922474	38.0	35.0	40.0	28.0	41.0
43	35.59942043268103	38.0	34.0	40.0	28.0	41.0
44	35.44292845745929	38.0	34.0	40.0	27.0	41.0
45	35.38928035102539	38.0	34.0	40.0	27.0	41.0
46	35.31746503824675	38.0	34.0	40.0	27.0	41.0
47	35.241874325402414	38.0	34.0	40.0	27.0	41.0
48	35.12052771129569	37.0	34.0	40.0	27.0	41.0
49	35.01594748697733	37.0	34.0	40.0	26.0	41.0
50	34.86711366089446	37.0	34.0	40.0	26.0	41.0
51	34.5093581350603	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	9.0
13	9.0
14	14.0
15	41.0
16	118.0
17	266.0
18	551.0
19	1268.0
20	2162.0
21	3416.0
22	5205.0
23	7536.0
24	10727.0
25	14988.0
26	19916.0
27	23532.0
28	26899.0
29	31441.0
30	38094.0
31	46431.0
32	58697.0
33	77205.0
34	129456.0
35	186793.0
36	118454.0
37	158310.0
38	255057.0
39	488030.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.407703317846924	24.094690037073537	29.489476277629173	12.008130367450374
2	30.612827913088363	26.029435919095217	29.892885635177624	13.464850532638792
3	31.091616218499222	25.822422450607725	29.69842554789056	13.387535783002487
4	27.82562532263363	27.061746210521378	29.275306208644235	15.837322258200759
5	24.699598761086865	31.655638462621425	28.86086864705054	14.783894129241165
6	24.468475761415366	40.47421277394528	25.03015157914496	10.027159885494392
7	89.86901074663288	3.6140245905485946	4.877340560326623	1.639624102491905
8	90.2968229386644	2.9403069125721526	5.02897836594866	1.733891782814773
9	83.87846684499507	6.447686423576893	6.715237692993571	2.958609038434464
10	43.0573935895631	36.07906283729879	12.374348866676051	8.489194706462058
11	34.51739875170116	21.986660565958047	26.91978741376883	16.576153268571964
12	33.82438171664555	21.052313576423106	27.557780749917875	17.56552395701347
13	26.123410296119008	28.0585668027594	27.484278943169553	18.333743957952038
14	18.622061100943263	31.715413674973014	29.41439063306584	20.24813459101788
15	17.347012999202217	28.693216481298983	37.181003331925474	16.778767187573326
16	23.38155239570135	25.89005819137454	34.189016378056216	16.539373034867896
17	23.261004739781313	25.57962597963302	27.024848655497678	24.13452062508799
18	23.056161715706978	26.77237317565348	30.96825285090807	19.203212257731476
19	26.23463090712844	28.063846262142754	26.37958139753156	19.32194143319724
20	28.54005349852175	28.10872166690131	26.446630531700222	16.904594302876717
21	26.808332160120134	26.46182364259233	29.21306724858041	17.51677694870712
22	25.531641559904266	22.85794734619175	29.67425735604674	21.936153737857246
23	21.762107560185836	26.43618893425313	30.28057393589563	21.5211295696654
24	21.957330236050495	25.010500258106905	33.44948143976724	19.582688066075367
25	21.57175371908583	27.475890468815994	30.91105870758834	20.04129710450983
26	20.41015533342719	33.089129006523066	26.07038105964616	20.430334600403587
27	19.69648974611666	31.67405790980337	29.75010558918767	18.8793467548923
28	18.216246656342392	29.298301187291752	33.19624337134544	19.289208785020413
29	19.915352667886808	27.45101834905439	32.76268243465202	19.870946548406778
30	21.3960650429396	28.590091041344035	29.759491294758085	20.25435262095828
31	25.382936787272982	27.371298512365666	26.954397203059738	20.29136749730161
32	26.449622225350787	27.789724998826788	27.1057417053827	18.65491107043972
33	25.367685015721058	27.226641325261625	27.33545684921864	20.070216809798676
34	20.61711014125487	27.470259045473743	30.054378431648598	21.85825238162279
35	21.700279224740722	27.283131540663568	29.47387254211835	21.542716692477356
36	24.885201088741844	27.157480407339623	28.248451358580883	19.70886714533765
37	21.88963583462387	29.252604533295788	28.78736684030222	20.070392791778122
38	21.97275798958187	29.803838753578297	26.40638931906706	21.81701393777277
39	20.828288516589236	28.690928715566194	28.540581444460088	21.940201323384485
40	23.265990895865595	26.5479374912009	28.414285043878174	21.77178656905533
41	20.250539678070297	25.43221174151767	29.561159603923226	24.756088976488808
42	20.846590642451545	26.59152236144352	27.884989910366514	24.67689708573842
43	21.047796705617344	27.241130508235955	28.148141630297058	23.562931155849643
44	21.116664320240275	28.068128490309256	28.97560889764888	21.839598291801586
45	20.002111783753342	30.296353653385893	27.294101084048993	22.407433478811768
46	21.74855694776855	29.530128114881038	27.80744051809095	20.913874419259468
47	20.903608803791826	27.8841686611291	28.772349711389555	22.439872823689523
48	21.64818855882491	26.59908958655967	30.066286545591066	21.686435309024358
49	21.574334788117696	25.69770988784082	30.598983997372002	22.128971326669483
50	20.103653385893285	28.271153033929323	29.59893706884415	22.02625651133324
51	19.507778403491482	29.299885025106764	27.62348068891079	23.568855882490965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	714.0
1	2003.5
2	3293.0
3	6127.5
4	8962.0
5	6648.5
6	4335.0
7	4132.5
8	3930.0
9	4069.0
10	4208.0
11	4196.0
12	4184.0
13	4118.0
14	4052.0
15	3922.5
16	3793.0
17	4072.5
18	4352.0
19	4627.0
20	4902.0
21	5571.0
22	6240.0
23	7671.5
24	9103.0
25	11610.0
26	16814.5
27	19512.0
28	23876.5
29	28241.0
30	33508.0
31	38775.0
32	44170.5
33	49566.0
34	55065.0
35	60564.0
36	64611.5
37	68659.0
38	76494.0
39	84329.0
40	95575.0
41	106821.0
42	118478.5
43	130136.0
44	135736.0
45	141336.0
46	141711.5
47	142087.0
48	138511.5
49	134936.0
50	129844.0
51	124752.0
52	114327.0
53	103902.0
54	94445.0
55	84988.0
56	81700.0
57	78412.0
58	73546.0
59	68680.0
60	61553.0
61	54426.0
62	47366.5
63	40307.0
64	33143.0
65	25979.0
66	21885.5
67	17792.0
68	14402.0
69	11012.0
70	8335.5
71	5659.0
72	4549.5
73	3440.0
74	2808.5
75	1523.5
76	870.0
77	802.0
78	734.0
79	472.0
80	210.0
81	166.5
82	123.0
83	86.5
84	50.0
85	41.5
86	33.0
87	23.0
88	13.0
89	11.5
90	10.0
91	5.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1704720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.058090284825024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95256027941655	16.237537628722773
2	6.927101623037088	2.778888595340718
3	2.056038540364845	1.2372062001515378
4	0.9844185647649859	0.7898222580045584
5	0.6274741809842623	0.6292966886789485
6	0.4191216842135948	0.5044068349370525
7	0.33367204950802487	0.4684976866188198
8	0.29257060400599666	0.46947260718704564
9	0.23797089117813972	0.4295917458370258
>10	4.085513882204237	21.5968087546467
>50	2.230840242934895	32.17919136031666
>100	0.8486150366303106	21.65616175504663
>500	0.0026372704867319648	0.34660344487646955
>1k	0.001465150270406647	0.6765144396350966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4836	0.28368295086583134	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2175	0.127586935097846	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1975	0.11585480313482566	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030210239804777324	0.0
2	0.0	0.0	0.0	0.10775963208034164	0.0
3	0.0	0.0	0.0	0.15697592566521187	0.0
4	0.0	0.0	0.0	0.2347012999202215	0.0
5	0.0	0.0	0.0	0.4472875310901497	0.0
6	0.0	0.0	0.0	0.6023863156412783	0.0
7	0.0	0.0	0.0	0.7090900558449481	0.0
8	0.0	0.0	0.0	0.9393918062790371	0.0
9	0.0	0.0	0.0	1.0055610305504716	0.0
10	0.0	0.0	0.0	1.1641207940306912	0.0
11	0.0	0.0	0.0	1.4351330423764606	0.0
12	0.0	0.0	0.0	1.644962222535079	0.0
13	0.0	0.0	0.0	1.7294922333286404	0.0
14	0.0	0.0	0.0	1.765040593176592	0.0
15	0.0	0.0	0.0	1.8356680275939743	0.0
16	0.0	0.0	0.0	1.9824956591111738	0.0
17	0.0	0.0	0.0	2.169095217983012	0.0
18	0.0	0.0	0.0	2.3676615514571306	0.0
19	0.0	0.0	0.0	2.5008212492374113	0.0
20	0.0	0.0	0.0	2.648294148012577	0.0
21	0.0	0.0	0.0	2.829672908160871	0.0
22	0.0	0.0	0.0	3.0309376319864847	0.0
23	0.0	0.0	0.0	3.228624055563377	0.0
24	0.0	0.0	0.0	3.3772115068750295	0.0
25	0.0	0.0	0.0	3.5171171805340466	0.0
26	0.0	0.0	0.0	3.6544417851611994	0.0
27	0.0	0.0	0.0	3.7990403116054248	0.0
28	0.0	0.0	0.0	3.9546670420948895	0.0
29	0.0	0.0	0.0	4.124841616218499	0.0
30	0.0	0.0	0.0	4.38758271153034	0.0
31	0.0	0.0	0.0	4.57858181988831	0.0
32	0.0	0.0	0.0	4.748052466094139	0.0
33	0.0	0.0	0.0	4.9274367638087195	0.0
34	0.0	0.0	0.0	5.1260617579426535	0.0
35	0.0	0.0	0.0	5.33747477591628	0.0
36	0.0	0.0	0.0	5.520554695199212	0.0
37	0.0	0.0	0.0	5.713724247970341	0.0
38	0.0	0.0	0.0	5.938394575062181	0.0
39	0.0	0.0	0.0	6.279740954526257	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	20	7.0342456E-4	45.000004	18
GTACGTC	20	7.0342456E-4	45.000004	25
GTACGAA	25	3.8916012E-5	45.0	25
TATAGCG	175	0.0	43.714287	1
CGTAAGG	275	0.0	43.363632	2
TAACGGG	390	0.0	41.538464	3
ATAACGG	130	0.0	41.538464	2
ATAGCGG	360	0.0	40.0	2
CGTTAAC	45	1.9292202E-8	40.0	37
TATCGCG	45	1.9292202E-8	40.0	1
CGGTCTA	445	0.0	39.4382	31
TAGGGAC	1725	0.0	39.391304	5
CTAGACG	80	0.0	39.375004	1
CTATCGG	40	3.459536E-7	39.375004	25
TCACGAC	440	0.0	39.375	25
CTCACGA	440	0.0	39.375	24
CACGACG	440	0.0	39.375	26
CGGGATC	605	0.0	39.049587	6
ACGTAAG	75	0.0	39.0	1
TCGTAAG	110	0.0	38.863636	1
>>END_MODULE