FastQCFastQC Report
Thu 26 May 2016
SRR1548107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548107_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1782057
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59570.33427662527068436No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26750.15010743202939075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG850.045.0000041
TACGATC302.1661763E-645.00000439
ACGTGCC351.2124292E-745.00000419
GTCGTGC351.2124292E-745.00000423
ATGCGTA351.2124292E-745.00000441
CGACTTA302.1661763E-645.00000414
GTAGTAT351.2124292E-745.0000049
GTTGCGA302.1661763E-645.0000049
CGTTCGA207.0343335E-445.029
TTATCGA207.0343335E-445.045
CATACCG253.8916733E-545.01
ACGCTCC207.0343335E-445.014
GCGATAC1000.045.09
TTACGAG551.8189894E-1245.01
TGTACGT406.8193913E-945.023
TTACCGA207.0343335E-445.022
GATTACG406.8193913E-945.01
CCGTTCA207.0343335E-445.029
ATCGACG207.0343335E-445.01
TTGTACG1150.043.043481