##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548107_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1782057
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48250252376888	31.0	31.0	33.0	30.0	34.0
2	31.875300846156996	31.0	31.0	34.0	30.0	34.0
3	31.96306515448159	33.0	31.0	34.0	30.0	34.0
4	35.679137648234594	37.0	35.0	37.0	33.0	37.0
5	35.56591736403493	37.0	35.0	37.0	33.0	37.0
6	35.6277627483296	37.0	35.0	37.0	33.0	37.0
7	36.03452583166532	37.0	35.0	37.0	35.0	37.0
8	36.07176818698841	37.0	35.0	37.0	35.0	37.0
9	37.86644366594334	39.0	38.0	39.0	35.0	39.0
10	37.24380308822894	39.0	37.0	39.0	34.0	39.0
11	36.905413238746014	39.0	37.0	39.0	33.0	39.0
12	36.15222577055616	38.0	35.0	39.0	32.0	39.0
13	35.8366023084559	38.0	35.0	39.0	31.0	39.0
14	36.809902825779425	39.0	35.0	41.0	31.0	41.0
15	37.06839960786888	39.0	35.0	41.0	32.0	41.0
16	37.228429281442736	39.0	35.0	41.0	32.0	41.0
17	37.1680709427364	39.0	35.0	41.0	32.0	41.0
18	37.13403611668987	38.0	35.0	41.0	32.0	41.0
19	37.087520769537676	38.0	35.0	41.0	32.0	41.0
20	36.96387545403991	38.0	35.0	41.0	32.0	41.0
21	36.826000515135036	38.0	35.0	41.0	32.0	41.0
22	36.74418046111881	38.0	35.0	41.0	31.0	41.0
23	36.67939970494771	38.0	35.0	40.0	31.0	41.0
24	36.71046605131037	38.0	35.0	41.0	31.0	41.0
25	36.68799033925402	38.0	35.0	41.0	31.0	41.0
26	36.53860398404765	38.0	35.0	40.0	31.0	41.0
27	36.452539958037256	38.0	35.0	40.0	31.0	41.0
28	36.458194098168576	38.0	35.0	40.0	31.0	41.0
29	36.421494935347184	38.0	35.0	40.0	31.0	41.0
30	36.34624762283137	38.0	35.0	40.0	31.0	41.0
31	36.25811463943072	38.0	35.0	40.0	30.0	41.0
32	36.14085688617143	38.0	35.0	40.0	30.0	41.0
33	36.061784219023295	38.0	35.0	40.0	30.0	41.0
34	35.96879112172057	38.0	35.0	40.0	30.0	41.0
35	35.86874718373206	38.0	35.0	40.0	30.0	41.0
36	35.69822457979739	38.0	35.0	40.0	29.0	41.0
37	35.62989511558833	38.0	34.0	40.0	29.0	41.0
38	35.55496204666854	38.0	34.0	40.0	29.0	41.0
39	35.516971679357056	38.0	34.0	40.0	29.0	41.0
40	35.391622153500144	38.0	34.0	40.0	27.0	41.0
41	35.34494126731075	38.0	34.0	40.0	27.0	41.0
42	35.3069711013733	37.0	34.0	40.0	27.0	41.0
43	35.209211040948745	37.0	34.0	40.0	27.0	41.0
44	35.040214763051914	37.0	34.0	40.0	27.0	41.0
45	35.011555185945234	37.0	34.0	40.0	27.0	41.0
46	34.922936247269305	37.0	34.0	40.0	26.0	41.0
47	34.84298538150014	37.0	34.0	40.0	26.0	41.0
48	34.73258206667912	36.0	34.0	40.0	26.0	41.0
49	34.63650377064258	36.0	34.0	40.0	26.0	41.0
50	34.459862956123175	36.0	34.0	40.0	24.0	41.0
51	34.08640632707035	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	2.0
13	4.0
14	10.0
15	35.0
16	117.0
17	265.0
18	583.0
19	1168.0
20	2160.0
21	3574.0
22	5761.0
23	8505.0
24	12277.0
25	17157.0
26	22587.0
27	26920.0
28	31183.0
29	36028.0
30	43906.0
31	53556.0
32	67776.0
33	87701.0
34	152456.0
35	242649.0
36	119554.0
37	153607.0
38	241206.0
39	451219.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.24738939326857	24.54736296313754	29.831425145211405	11.373822498382486
2	30.302509964608316	25.703442706939228	30.287807853508614	13.706239474943843
3	29.22667456764851	25.852315610555664	31.13519937914444	13.785810442651385
4	27.185269606976657	27.752703757511682	30.123503344730274	14.938523290781383
5	23.417937810070047	31.60746261202644	30.0364129766893	14.938186601214214
6	22.089865812373006	41.2888027711796	26.398650548214786	10.22268086823261
7	89.3136414828482	3.7833806662749847	5.20297611131406	1.7000017395627638
8	90.37589706726553	2.688353963986562	4.980087617848363	1.9556613508995502
9	84.79122721663785	5.909238593378326	6.474428146798895	2.825106043184926
10	46.832284264756964	29.21056958335227	13.736934340484058	10.220211811406706
11	39.84726638934669	22.029654494777663	23.02322540749258	15.099853708383066
12	35.756039228823774	22.073592483293183	25.379378998539327	16.79098928934372
13	23.37714225751477	34.58492068435521	26.250394908804825	15.78754214932519
14	17.47620867345994	37.5612003431989	28.509806364218427	16.45278461912273
15	16.387298498308414	27.244975890221244	41.1832505918722	15.184475019598139
16	19.237992948598166	23.41288746656252	39.863932522921544	17.485187061917774
17	19.79375519413801	23.631679570294327	27.256198875793537	29.318366359774128
18	22.634292842484836	25.764383518596766	32.39396944093259	19.20735419798581
19	27.456753627970375	27.72318730545656	25.779534549119358	19.040524517453704
20	28.76793503238112	26.422555507483768	26.021951037480846	18.787558422654268
21	23.78324599044812	28.288994123083604	29.351193592573075	18.5765662938952
22	23.191626306004803	23.97891874390101	28.38955207381133	24.439902876282858
23	19.907275693201733	29.68962272250551	27.065183661353142	23.337917922939614
24	19.90188866012703	26.23664675147877	35.10084133111342	18.760623257280773
25	19.029301531881416	27.366913628464186	32.84378670267	20.759998136984397
26	18.90377243825534	33.82512456111112	26.99055080729741	20.280552193336128
27	18.363497912805258	34.26298934321405	29.229929233464475	18.14358351051622
28	16.84042654078966	29.388510019600943	35.8066548937548	17.964408545854592
29	17.597080228073516	27.247669406758597	35.10381542229008	20.051434942877812
30	19.2624029422179	30.11547891004609	31.341982888313897	19.280135259422117
31	26.579172271145087	27.208669531894884	27.40333221664627	18.808825980313763
32	27.62784804301995	27.28117001869188	26.97893501723009	18.11204692105808
33	25.414058023957708	27.829132289258986	26.532091846669324	20.224717840113982
34	19.29713808256414	28.479335958389658	28.995873869354348	23.227652089691855
35	20.2725838735798	26.28108977434504	30.854793084620752	22.59153326745441
36	27.07275917661444	25.23319961146024	28.449595046623088	19.244446165302232
37	19.587140029752135	30.963936619311276	30.34201487382278	19.10690847711381
38	19.64650962342955	31.318190158900645	27.060638352196364	21.97466186547344
39	19.629338455503948	30.0582416836274	29.723852828500995	20.588567032367653
40	23.386962369890526	26.550946462430776	27.253617589111904	22.80847357856679
41	18.393968318634027	24.477668222733616	29.410058151899744	27.71830530673261
42	20.62397555184823	25.59457974688801	28.697623027770717	25.083821673493045
43	21.36278469207214	25.781274111883068	28.477203591130923	24.378737604913873
44	19.72501440750773	29.60444026201182	28.765578205410936	21.904967125069515
45	18.697830653003805	33.792858477590784	25.626509140841176	21.88280172856424
46	21.687970699029268	30.874152734732952	27.189702686277712	20.248173879960067
47	20.336723236125444	27.680708305065437	28.365085965263738	23.61748249354538
48	21.051683532008237	25.666406854550665	31.996282947178457	21.28562666626264
49	21.186022669308556	24.6421971912234	31.735741337117723	22.436038802350318
50	19.56531132281403	30.524500619228228	28.48057048680261	21.42961757115513
51	18.863425805122958	31.2758795032931	26.948015691978426	22.91267899960551
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	623.0
1	1608.5
2	2594.0
3	6214.5
4	9835.0
5	7285.0
6	4735.0
7	4610.0
8	4485.0
9	4928.0
10	5371.0
11	5545.0
12	5719.0
13	5599.5
14	5480.0
15	5403.0
16	5326.0
17	4805.0
18	4284.0
19	4654.0
20	5024.0
21	6041.0
22	7058.0
23	6949.0
24	6840.0
25	9694.0
26	15052.0
27	17556.0
28	20992.5
29	24429.0
30	29457.0
31	34485.0
32	38009.0
33	41533.0
34	48829.5
35	56126.0
36	60886.5
37	65647.0
38	76377.5
39	87108.0
40	107876.5
41	128645.0
42	152204.0
43	175763.0
44	177273.5
45	178784.0
46	174360.0
47	169936.0
48	164909.5
49	159883.0
50	151544.5
51	143206.0
52	123928.5
53	104651.0
54	95641.5
55	86632.0
56	76283.5
57	65935.0
58	58505.0
59	51075.0
60	45187.5
61	39300.0
62	33138.0
63	26976.0
64	22980.0
65	18984.0
66	14900.0
67	10816.0
68	8633.0
69	6450.0
70	4952.0
71	3454.0
72	2789.0
73	2124.0
74	1778.5
75	969.0
76	505.0
77	411.5
78	318.0
79	273.5
80	229.0
81	146.5
82	64.0
83	51.0
84	38.0
85	28.0
86	18.0
87	18.5
88	19.0
89	11.0
90	3.0
91	5.0
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1782057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.48022794022988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87926236737994	17.158247633487473
2	8.787140427179622	3.774995586372544
3	2.909445574136892	1.874866623364603
4	1.3605959099012714	1.1690364111689513
5	0.8134731393797304	0.8736794228565501
6	0.5119400809341818	0.6597953778123574
7	0.38681066173077167	0.5816146828581692
8	0.3123191780971962	0.536694970850642
9	0.23488556543103298	0.45408559367955315
>10	2.497399285777727	12.543666453107011
>50	1.043896155696799	16.512428024307418
>100	1.2562844143389649	42.522482556588926
>500	0.005761571214009132	0.7795183732664281
>1k	5.237792012735574E-4	0.2237803340444433
>5k	2.618896006367787E-4	0.3351079562349796
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5957	0.33427662527068436	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2675	0.15010743202939075	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.611492786145449E-5	0.0	0.0	0.05993074295603339	0.0
2	5.611492786145449E-5	0.0	0.0	0.22900502060259575	0.0
3	5.611492786145449E-5	0.0	0.0	0.3319759132283647	0.0
4	5.611492786145449E-5	0.0	0.0	0.4862919648473646	0.0
5	5.611492786145449E-5	0.0	0.0	0.8703986460590205	0.0
6	5.611492786145449E-5	0.0	0.0	1.1765616924711162	0.0
7	5.611492786145449E-5	0.0	0.0	1.3725711354911767	0.0
8	5.611492786145449E-5	0.0	0.0	1.8472473102712204	0.0
9	5.611492786145449E-5	0.0	0.0	1.9710929560614503	0.0
10	5.611492786145449E-5	0.0	0.0	2.2587941912071274	0.0
11	5.611492786145449E-5	0.0	0.0	2.724323632745754	0.0
12	5.611492786145449E-5	0.0	0.0	3.0589930625114685	0.0
13	5.611492786145449E-5	0.0	0.0	3.1967552104113395	0.0
14	5.611492786145449E-5	0.0	0.0	3.252870138272794	0.0
15	5.611492786145449E-5	0.0	0.0	3.3571877891672375	0.0
16	5.611492786145449E-5	0.0	0.0	3.617897744011555	0.0
17	5.611492786145449E-5	0.0	0.0	3.9243974799908194	0.0
18	5.611492786145449E-5	0.0	0.0	4.275733043331386	0.0
19	5.611492786145449E-5	0.0	0.0	4.481843173366508	0.0
20	5.611492786145449E-5	0.0	0.0	4.688177763113076	0.0
21	5.611492786145449E-5	0.0	0.0	4.984464582221556	0.0
22	5.611492786145449E-5	0.0	0.0	5.30134557985519	0.0
23	5.611492786145449E-5	0.0	0.0	5.661435071942143	0.0
24	5.611492786145449E-5	0.0	0.0	5.911763765132092	0.0
25	5.611492786145449E-5	0.0	0.0	6.137233545279416	0.0
26	5.611492786145449E-5	0.0	0.0	6.361188222374481	0.0
27	5.611492786145449E-5	0.0	0.0	6.588958714564124	0.0
28	5.611492786145449E-5	0.0	0.0	6.8310946282863005	0.0
29	5.611492786145449E-5	0.0	0.0	7.086810354550948	0.0
30	5.611492786145449E-5	0.0	0.0	7.395723032428256	0.0
31	5.611492786145449E-5	0.0	0.0	7.684714910914746	0.0
32	5.611492786145449E-5	0.0	0.0	7.943460843283913	0.0
33	5.611492786145449E-5	0.0	0.0	8.211634083533804	0.0
34	5.611492786145449E-5	0.0	0.0	8.479695093927972	0.0
35	5.611492786145449E-5	0.0	0.0	8.80673289350453	0.0
36	5.611492786145449E-5	0.0	0.0	9.088317601513307	0.0
37	5.611492786145449E-5	0.0	0.0	9.373774239544526	0.0
38	5.611492786145449E-5	0.0	0.0	9.684202020474093	0.0
39	5.611492786145449E-5	0.0	0.0	10.057478520608488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	85	0.0	45.000004	1
TACGATC	30	2.1661763E-6	45.000004	39
ACGTGCC	35	1.2124292E-7	45.000004	19
GTCGTGC	35	1.2124292E-7	45.000004	23
ATGCGTA	35	1.2124292E-7	45.000004	41
CGACTTA	30	2.1661763E-6	45.000004	14
GTAGTAT	35	1.2124292E-7	45.000004	9
GTTGCGA	30	2.1661763E-6	45.000004	9
CGTTCGA	20	7.0343335E-4	45.0	29
TTATCGA	20	7.0343335E-4	45.0	45
CATACCG	25	3.8916733E-5	45.0	1
ACGCTCC	20	7.0343335E-4	45.0	14
GCGATAC	100	0.0	45.0	9
TTACGAG	55	1.8189894E-12	45.0	1
TGTACGT	40	6.8193913E-9	45.0	23
TTACCGA	20	7.0343335E-4	45.0	22
GATTACG	40	6.8193913E-9	45.0	1
CCGTTCA	20	7.0343335E-4	45.0	29
ATCGACG	20	7.0343335E-4	45.0	1
TTGTACG	115	0.0	43.04348	1
>>END_MODULE