FastQCFastQC Report
Thu 26 May 2016
SRR1548106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548106_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1937248
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186620.963325294438296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG207.034485E-445.0000041
CGTTTTT88400.042.0984151
CGGTCTA2750.041.72727231
TAACGCC556.184564E-1140.90909212
CTATGCG451.929402E-840.01
CGCATAT356.250506E-638.5714336
CGCTATC301.14021095E-437.49999623
GGCGATA5550.037.29738
CGAGGGT1700.037.0588234
GGGCGAT25950.036.9364177
AGGGCGA12200.036.8852466
TAGGGAT33500.036.8731355
AAGGGAT32250.036.558145
ACGGGAT10400.036.3461535
ATAGGGA28750.036.313044
AGGGATC21250.036.2117656
TACGCGG1500.036.0000042
GCGTAGT250.002107464536.032
TCGTCCC4700.035.90425538
CGAGGGA13450.035.7992554
Produced by FastQC (version 0.11.5)