##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548106_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1937248
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55267833545318	31.0	31.0	33.0	30.0	34.0
2	31.961079324897998	31.0	31.0	34.0	30.0	34.0
3	31.954388390128678	33.0	31.0	34.0	30.0	34.0
4	35.73806193115182	37.0	35.0	37.0	35.0	37.0
5	35.705916846991194	37.0	35.0	37.0	33.0	37.0
6	35.77971483258726	37.0	35.0	37.0	35.0	37.0
7	36.06193579758503	37.0	35.0	37.0	35.0	37.0
8	36.076114157815624	37.0	35.0	37.0	35.0	37.0
9	37.88882941161896	39.0	38.0	39.0	35.0	39.0
10	37.41977330728952	39.0	37.0	39.0	35.0	39.0
11	37.16700804440113	39.0	37.0	39.0	33.0	39.0
12	36.812266679330676	39.0	35.0	39.0	33.0	39.0
13	36.70338800194916	39.0	35.0	39.0	33.0	39.0
14	37.82392961561968	40.0	36.0	41.0	33.0	41.0
15	37.9361620195246	40.0	36.0	41.0	33.0	41.0
16	37.98951141000016	40.0	36.0	41.0	33.0	41.0
17	37.907980805761575	40.0	36.0	41.0	33.0	41.0
18	37.772760637770695	39.0	36.0	41.0	33.0	41.0
19	37.68602213036225	39.0	36.0	41.0	33.0	41.0
20	37.55080867292159	39.0	35.0	41.0	33.0	41.0
21	37.45360015857546	39.0	35.0	41.0	32.0	41.0
22	37.379668994367265	39.0	35.0	41.0	32.0	41.0
23	37.264178360230595	39.0	35.0	41.0	32.0	41.0
24	37.26408234774278	39.0	35.0	41.0	32.0	41.0
25	37.24315007680999	39.0	35.0	41.0	32.0	41.0
26	37.13350691289912	39.0	35.0	41.0	32.0	41.0
27	37.07586586745734	39.0	35.0	41.0	32.0	41.0
28	36.99355683939279	39.0	35.0	41.0	32.0	41.0
29	36.91064953996597	39.0	35.0	41.0	31.0	41.0
30	36.78255133054725	39.0	35.0	41.0	31.0	41.0
31	36.696550467467254	39.0	35.0	41.0	31.0	41.0
32	36.53876788103537	39.0	35.0	41.0	30.0	41.0
33	36.37491689241646	39.0	35.0	41.0	30.0	41.0
34	36.1645199788566	39.0	35.0	41.0	29.0	41.0
35	35.97268031764648	39.0	35.0	41.0	29.0	41.0
36	35.82957215596558	39.0	35.0	41.0	27.0	41.0
37	35.77145259667322	39.0	35.0	41.0	27.0	41.0
38	35.6523165851765	38.0	35.0	41.0	27.0	41.0
39	35.56889463816713	38.0	35.0	41.0	26.0	41.0
40	35.4413317241778	38.0	35.0	40.0	26.0	41.0
41	35.352800467467254	38.0	35.0	40.0	25.0	41.0
42	35.2954247468574	38.0	34.0	40.0	25.0	41.0
43	35.19843768066866	38.0	34.0	40.0	25.0	41.0
44	35.029383950841606	38.0	34.0	40.0	24.0	41.0
45	34.99227099473067	38.0	34.0	40.0	24.0	41.0
46	34.89411371182213	38.0	34.0	40.0	24.0	41.0
47	34.819444903285486	38.0	34.0	40.0	23.0	41.0
48	34.693228228910286	38.0	34.0	40.0	23.0	41.0
49	34.5747963089909	37.0	34.0	40.0	24.0	41.0
50	34.45123133434645	37.0	34.0	40.0	23.0	41.0
51	34.09160778589009	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	6.0
12	17.0
13	16.0
14	25.0
15	63.0
16	139.0
17	321.0
18	689.0
19	1456.0
20	2558.0
21	4139.0
22	6297.0
23	9366.0
24	14049.0
25	21078.0
26	29129.0
27	33534.0
28	35068.0
29	37596.0
30	42735.0
31	50655.0
32	61828.0
33	79439.0
34	126781.0
35	173748.0
36	139927.0
37	188171.0
38	301792.0
39	576527.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.32149523447695	23.714465055584004	28.28961495895208	14.674424750986967
2	33.91527569005104	24.59607649614298	28.211566097887314	13.277081715918666
3	29.409037975519915	24.544611737887973	32.68110226465584	13.36524802193627
4	26.221268521118617	26.945336890269083	31.924668395579708	14.90872619303259
5	23.198785080691785	31.089359751565105	30.40607087992864	15.305784287814467
6	22.101145542542824	39.4740373973802	28.223799947141515	10.201017112935464
7	86.19701762500208	3.8040044434166407	8.18862634004526	1.8103515915360346
8	86.50823229653611	3.3515584994796748	8.33032218900213	1.8098870149820776
9	80.44401129850179	6.5642086093262195	10.048171426683625	2.943608665488363
10	36.75703885098862	37.3270742826938	16.154462412659605	9.761424453657973
11	29.683499479674257	23.963994284675994	29.985267761277857	16.36723847437189
12	28.741080130164026	22.275206891425363	31.580159071012076	17.403553907398535
13	24.57006020912139	24.939759906836915	31.95739523282512	18.532784651216573
14	19.433701828573316	28.232007466261415	32.57920514048795	19.755085564677316
15	18.909298138390128	27.39716339880077	35.79118419531211	17.902354267496985
16	24.106877384826312	25.902052808933085	33.32867036125473	16.662399444985876
17	23.54566890764631	25.66016328317283	30.314910636118864	20.479257173061992
18	23.480576570475233	26.116325839541453	31.892973950676424	18.510123639306894
19	24.64237929268736	27.283032425378686	29.349417317762104	18.725170964171856
20	26.272423561670987	26.96366185434183	29.511812633178614	17.25210195080857
21	25.732172649036155	26.456705594740583	31.29640603577859	16.514715720444674
22	24.636236558251706	23.73934571102925	32.068170931135306	19.55624679958374
23	22.337356910421384	26.078695138670938	32.104253043492626	19.479694907415055
24	21.571373412180577	26.140277341878786	33.53251622920762	18.755833016733014
25	21.957901105072764	27.301667107154064	30.74082409686318	19.999607690909993
26	20.883593633855863	29.108715043195293	29.62057516642165	20.387116156527195
27	19.764790052693304	28.835544029468608	31.80134913031269	19.5983167875254
28	18.705555509671452	28.13904053585292	33.43655536100695	19.718848593468675
29	20.632154478930936	26.978425064834237	32.53300558317779	19.856414873057037
30	21.314720675927916	27.201628289202002	32.24212904078363	19.241521994086458
31	23.7437849981004	26.477456680817323	30.315426832289926	19.46333148879235
32	24.678461405044683	26.886129189448127	29.761909678058775	18.673499727448423
33	23.826118287384993	26.742729893126743	29.479240654784515	19.951911164703745
34	21.053628652604107	27.098285815755137	31.87217124498257	19.975914286658185
35	21.16956631262492	28.096093014420454	30.668195295594575	20.066145377360048
36	22.993029286905962	29.121929665174516	28.862799187300748	19.022241860618774
37	22.152971638117577	29.353288789045077	29.028859495531805	19.464880077305537
38	21.42947108475528	30.024756768364195	28.542551082773088	20.003221064107436
39	21.247924891392326	29.006766299410298	28.7914608764598	20.953847932737574
40	22.74969441186673	27.528173987016636	28.958398718181666	20.763732882934967
41	20.285993326615902	27.26460422207172	30.082209402203535	22.367193049108838
42	20.601957002923736	27.946086407109465	28.60470110176911	22.84725548819769
43	20.765591189150793	27.570708551512247	28.95793414162771	22.705766117709246
44	20.638193974132378	27.998841655792134	29.141545119674923	22.22141925040057
45	20.53469664183419	29.230847057268868	28.299474305819388	21.934981995077553
46	21.54432473281686	29.24602322469813	28.567173640132808	20.6424784023522
47	20.40760914451841	28.277071391995246	30.378351145542542	20.936968317943805
48	20.711235732337833	27.660010489106195	30.59783775747865	21.030916021077324
49	20.90991963858009	27.456164621153306	30.411387700490593	21.222528039776012
50	20.29864013280695	28.06682469152117	30.786507458002276	20.848027717669602
51	19.964867688597433	28.41819942516394	29.793346024876527	21.823586861362095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	905.0
1	2101.5
2	3298.0
3	17301.0
4	31304.0
5	22436.0
6	13568.0
7	12277.5
8	10987.0
9	11006.5
10	11026.0
11	10779.5
12	10533.0
13	10254.5
14	9976.0
15	9610.5
16	9245.0
17	9044.0
18	8843.0
19	8494.0
20	8145.0
21	9755.0
22	11365.0
23	12216.0
24	13067.0
25	16364.5
26	23750.5
27	27839.0
28	32510.5
29	37182.0
30	41086.0
31	44990.0
32	53698.0
33	62406.0
34	68925.0
35	75444.0
36	78533.5
37	81623.0
38	87920.0
39	94217.0
40	101827.0
41	109437.0
42	118192.0
43	126947.0
44	129739.5
45	132532.0
46	134899.0
47	137266.0
48	137736.5
49	138207.0
50	134493.0
51	130779.0
52	122755.0
53	114731.0
54	104309.0
55	93887.0
56	89170.0
57	84453.0
58	80475.5
59	76498.0
60	70670.0
61	64842.0
62	57039.0
63	49236.0
64	42034.5
65	34833.0
66	29565.5
67	24298.0
68	19156.0
69	14014.0
70	11071.5
71	8129.0
72	6581.5
73	5034.0
74	4071.0
75	2443.5
76	1779.0
77	1307.5
78	836.0
79	607.0
80	378.0
81	280.5
82	183.0
83	136.0
84	89.0
85	74.0
86	59.0
87	43.0
88	27.0
89	20.5
90	14.0
91	14.5
92	15.0
93	11.5
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1937248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.39258135036263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26518856577607	18.30824790426388
2	5.945598248374337	2.7816578140334043
3	2.177545845566839	1.528152550096994
4	1.2898408396797199	1.2069082708491157
5	0.8969255867960281	1.049070237717391
6	0.7030760999357227	0.9868058917945248
7	0.5798022304980125	0.9494149590832524
8	0.5160308326951776	0.9657034586493849
9	0.45684995628121217	0.9618209790496081
>10	7.661192488245687	43.82975464902455
>50	1.3498532813441801	21.3039929786003
>100	0.15565009656179546	4.726976298735012
>500	0.0015564997923066192	0.23214325111943415
>1k	6.670713395599796E-4	0.19864554694714745
>5k	0.0	0.0
>10k+	2.2235711318665987E-4	0.9707052100360476
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18662	0.963325294438296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.161961710632815E-5	0.0	0.0	0.023951502337336263	0.0
2	5.161961710632815E-5	0.0	0.0	0.06385346636052792	0.0
3	5.161961710632815E-5	0.0	0.0	0.10943358826541569	0.0
4	5.161961710632815E-5	0.0	0.0	0.15444589438213382	0.0
5	5.161961710632815E-5	0.0	0.0	0.2788491716083847	0.0
6	5.161961710632815E-5	0.0	0.0	0.46075670229108506	0.0
7	5.161961710632815E-5	0.0	0.0	0.5805142139777664	0.0
8	5.161961710632815E-5	0.0	0.0	0.7877153570425676	0.0
9	5.161961710632815E-5	0.0	0.0	0.9119121558003931	0.0
10	5.161961710632815E-5	0.0	0.0	1.0917549017988404	0.0
11	5.161961710632815E-5	0.0	0.0	1.2886837410594822	0.0
12	5.161961710632815E-5	0.0	0.0	1.447981879449611	0.0
13	5.161961710632815E-5	0.0	0.0	1.5270631328565056	0.0
14	5.161961710632815E-5	0.0	0.0	1.5724883959100744	0.0
15	5.161961710632815E-5	0.0	0.0	1.6198752044136837	0.0
16	5.161961710632815E-5	0.0	0.0	1.7120162209484795	0.0
17	5.161961710632815E-5	0.0	0.0	1.8212949503625762	0.0
18	5.161961710632815E-5	0.0	0.0	1.9466790003138472	0.0
19	5.161961710632815E-5	0.0	0.0	2.0289606699813345	0.0
20	5.161961710632815E-5	0.0	0.0	2.1209984472819174	0.0
21	5.161961710632815E-5	0.0	0.0	2.2292964039709937	0.0
22	5.161961710632815E-5	0.0	0.0	2.3574162936289005	0.0
23	5.161961710632815E-5	0.0	0.0	2.486981532565784	0.0
24	5.161961710632815E-5	0.0	0.0	2.5925952691653316	0.0
25	5.161961710632815E-5	0.0	0.0	2.690569302433142	0.0
26	5.161961710632815E-5	0.0	0.0	2.786323692165381	0.0
27	1.032392342126563E-4	0.0	0.0	2.8908534168056956	0.0
28	1.032392342126563E-4	0.0	0.0	2.9962606749368175	0.0
29	1.032392342126563E-4	0.0	0.0	3.1121983349576308	0.0
30	1.032392342126563E-4	0.0	0.0	3.2585399494540708	0.0
31	1.032392342126563E-4	0.0	0.0	3.3855758271527443	0.0
32	1.032392342126563E-4	0.0	0.0	3.5156056426435853	0.0
33	1.032392342126563E-4	0.0	0.0	3.6492488313318687	0.0
34	1.032392342126563E-4	0.0	0.0	3.792183551099291	0.0
35	1.032392342126563E-4	0.0	0.0	3.937905730190456	0.0
36	1.032392342126563E-4	0.0	0.0	4.08109854804341	0.0
37	1.032392342126563E-4	0.0	0.0	4.232137547696526	0.0
38	1.032392342126563E-4	0.0	0.0	4.416935776937181	0.0
39	1.032392342126563E-4	0.0	0.0	4.6689943672673815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAACG	20	7.034485E-4	45.000004	1
CGTTTTT	8840	0.0	42.098415	1
CGGTCTA	275	0.0	41.727272	31
TAACGCC	55	6.184564E-11	40.909092	12
CTATGCG	45	1.929402E-8	40.0	1
CGCATAT	35	6.250506E-6	38.57143	36
CGCTATC	30	1.14021095E-4	37.499996	23
GGCGATA	555	0.0	37.2973	8
CGAGGGT	170	0.0	37.058823	4
GGGCGAT	2595	0.0	36.936417	7
AGGGCGA	1220	0.0	36.885246	6
TAGGGAT	3350	0.0	36.873135	5
AAGGGAT	3225	0.0	36.55814	5
ACGGGAT	1040	0.0	36.346153	5
ATAGGGA	2875	0.0	36.31304	4
AGGGATC	2125	0.0	36.211765	6
TACGCGG	150	0.0	36.000004	2
GCGTAGT	25	0.0021074645	36.0	32
TCGTCCC	470	0.0	35.904255	38
CGAGGGA	1345	0.0	35.799255	4
>>END_MODULE