FastQCFastQC Report
Thu 26 May 2016
SRR1548105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548105_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1204108
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41920.34814152883296184No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20270.1683403814275796No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18580.15430509555621258No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17370.1442561630684291No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG302.1655887E-645.00000416
AACCGAG302.1655887E-645.0000041
GGTCGAC207.0334197E-445.0000049
ACGCATG207.0334197E-445.0000041
CGTTGCC207.0334197E-445.00000420
CACGCTA207.0334197E-445.00000430
CCGTGTG207.0334197E-445.00000413
ATTCGGT207.0334197E-445.00000437
CGATTAC207.0334197E-445.00000424
ATCGTTC302.1655887E-645.00000419
CCGTCCC207.0334197E-445.00000410
CTAACGG800.045.0000042
TCGGACG207.0334197E-445.00000427
CGTAGTG207.0334197E-445.00000418
TCGCTGA207.0334197E-445.00000415
CCGAGTT302.1655887E-645.00000443
TCGCTAG207.0334197E-445.0000041
TCCCGTA207.0334197E-445.00000443
TCCGTAA207.0334197E-445.00000432
TCGTAGC207.0334197E-445.00000424