##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548105_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1204108
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54672919704877	31.0	31.0	33.0	30.0	34.0
2	31.947862650194168	31.0	31.0	34.0	30.0	34.0
3	32.032186481611284	33.0	31.0	34.0	30.0	34.0
4	35.73845784597395	37.0	35.0	37.0	35.0	37.0
5	35.636820783517756	37.0	35.0	37.0	33.0	37.0
6	35.70327661638325	37.0	35.0	37.0	33.0	37.0
7	36.06254422360785	37.0	35.0	37.0	35.0	37.0
8	36.07564022496321	37.0	35.0	37.0	35.0	37.0
9	37.881219957013826	39.0	38.0	39.0	35.0	39.0
10	37.36404126540144	39.0	37.0	39.0	35.0	39.0
11	37.00454029040584	39.0	37.0	39.0	33.0	39.0
12	36.08311795951858	37.0	35.0	39.0	32.0	39.0
13	35.66344298019779	37.0	35.0	39.0	31.0	39.0
14	36.66292724572879	38.0	35.0	41.0	31.0	41.0
15	36.94533380726646	38.0	35.0	41.0	32.0	41.0
16	37.13460337444814	38.0	35.0	41.0	32.0	41.0
17	37.088409013145004	38.0	35.0	41.0	32.0	41.0
18	37.04486308537108	38.0	35.0	41.0	32.0	41.0
19	36.96911406618011	38.0	35.0	41.0	32.0	41.0
20	36.84776780820325	38.0	35.0	41.0	32.0	41.0
21	36.65549851010042	38.0	35.0	41.0	32.0	41.0
22	36.572663747770136	38.0	35.0	41.0	31.0	41.0
23	36.527673597384954	38.0	35.0	40.0	31.0	41.0
24	36.5448473060556	38.0	35.0	40.0	31.0	41.0
25	36.50068847644896	38.0	35.0	40.0	31.0	41.0
26	36.36689399954157	38.0	35.0	40.0	31.0	41.0
27	36.30723656017567	38.0	35.0	40.0	31.0	41.0
28	36.304838934713494	38.0	35.0	40.0	31.0	41.0
29	36.30671169031349	38.0	35.0	40.0	31.0	41.0
30	36.258632116056035	38.0	35.0	40.0	31.0	41.0
31	36.117037674361434	38.0	35.0	40.0	30.0	41.0
32	35.98974095346929	38.0	35.0	40.0	30.0	41.0
33	35.89278619525823	38.0	35.0	40.0	30.0	41.0
34	35.8181649818787	38.0	35.0	40.0	30.0	41.0
35	35.713918518936836	37.0	35.0	40.0	30.0	41.0
36	35.52759303982699	37.0	35.0	40.0	29.0	41.0
37	35.442682882266375	37.0	34.0	40.0	29.0	41.0
38	35.41062512664977	37.0	34.0	40.0	29.0	41.0
39	35.41470034249419	37.0	34.0	40.0	29.0	41.0
40	35.258774960385615	37.0	34.0	40.0	28.0	41.0
41	35.24474133549482	37.0	34.0	40.0	28.0	41.0
42	35.202872167612874	37.0	34.0	40.0	28.0	41.0
43	35.071721141292976	36.0	34.0	40.0	27.0	41.0
44	34.93209247011065	36.0	34.0	40.0	27.0	41.0
45	34.90692612290592	36.0	34.0	40.0	27.0	41.0
46	34.832338129137916	36.0	34.0	40.0	27.0	41.0
47	34.77104047145273	36.0	34.0	40.0	27.0	41.0
48	34.68218465453265	36.0	34.0	40.0	26.0	41.0
49	34.61404209589173	36.0	34.0	40.0	26.0	41.0
50	34.465119407893646	35.0	34.0	40.0	26.0	41.0
51	34.08952187013125	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	7.0
12	7.0
13	4.0
14	5.0
15	25.0
16	80.0
17	220.0
18	460.0
19	907.0
20	1634.0
21	2558.0
22	3983.0
23	5820.0
24	8452.0
25	11641.0
26	15297.0
27	18580.0
28	20885.0
29	23782.0
30	28832.0
31	35426.0
32	45017.0
33	59799.0
34	110419.0
35	190666.0
36	74848.0
37	95072.0
38	151666.0
39	297907.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.137060795211056	24.760237453783215	29.14788374464749	12.954818006358234
2	29.314563145498578	27.119245117547596	30.248366425603017	13.31782531135081
3	30.39694113817033	25.387008474322904	30.449345075358693	13.766705312148082
4	27.60300571045122	27.3776106462211	30.27510821288456	14.744275430443116
5	23.914549193261735	33.045374667388636	28.284921286130484	14.755154853219146
6	22.526799921601718	41.871078009613754	25.40444877037608	10.197673298408448
7	89.21708019546419	4.016251033960408	5.166895328326031	1.5997734422493664
8	89.627342397858	3.7135373238945344	4.920654957860923	1.7384653203865434
9	84.21694731701808	6.939825995674806	6.203679404173048	2.639547283134071
10	49.50660571975271	31.212233454141984	10.663744448172423	8.617416377932877
11	44.983008168702476	19.923960309208145	21.263541144149862	13.829490377939521
12	41.549346071947035	20.957090227786875	23.59763409926684	13.895929600999246
13	22.742146053344054	38.75391576170909	23.029080447933243	15.474857737013622
14	15.996654785118944	41.57658615340152	26.377700339172232	16.0490587223073
15	15.040843512375968	26.70865071903849	44.54700076737302	13.703505001212516
16	17.15028884452225	22.160470655456155	43.54243971470997	17.146800785311616
17	17.364721436947516	21.99022014636561	27.33259807259814	33.312460344088734
18	21.89678998893787	26.44995299424969	32.28996070119956	19.363296315612885
19	27.738043431320115	26.416982529806297	25.367907197693228	20.47706684118036
20	31.612529773076837	24.34914476110116	25.5513625023669	18.486962963455106
21	23.900596956419193	28.889767363060457	27.80365216409159	19.40598351642876
22	23.6146591501759	25.133044544177103	25.68606802712049	25.56622827852651
23	20.14470462782408	30.341132190800163	25.657250014118333	23.85691316725742
24	20.53860617153943	25.809894129098055	36.32365203121315	17.327847668149367
25	18.095635939633322	27.70748138871264	33.23289937447472	20.963983297179322
26	16.977048570394018	36.52346799456527	26.295066555491697	20.20441687954901
27	18.875217173210377	35.397904506904695	28.690947988054226	17.035930331830702
28	15.425277466805303	30.43173868124786	37.59720888823926	16.545774963707576
29	15.779896819886588	26.559494663269405	36.87775515153126	20.782853365312747
30	18.31264305195215	31.58819640763121	31.279835363605258	18.819325176811383
31	28.81568762934886	28.265238666299037	24.189773674786647	18.729300029565454
32	28.753982200932143	27.96393678972318	26.584741568032104	16.69733944131257
33	26.273556857026115	29.795333973364514	24.170506300099326	19.760602869510045
34	19.033010327977223	29.5686101246732	27.851654502752243	23.546725044597327
35	21.249173662163194	27.077637554106442	29.971647061559263	21.7015417221711
36	28.60806505728722	26.046749959305977	27.588389081378082	17.75679590202872
37	20.4186003248878	32.71882588605009	29.314397047440927	17.548176741621184
38	19.145707860092283	33.324419404239485	25.031973876097492	22.497898859570736
39	20.47158560527793	31.497008573981738	28.198384198095187	19.833021622645145
40	23.57853282263717	27.806060585927504	25.92715935779847	22.68824723363685
41	17.714357848299322	25.371229158846216	29.35052337497965	27.56388961787481
42	22.784334959986978	25.908224179226448	25.676517388805653	25.63092347198092
43	22.298165945247437	26.28044992641856	26.175309855926542	25.24607427240746
44	19.06240968418115	30.825391077876734	28.78479339062609	21.327405847316022
45	18.746657276589808	36.35820042720421	23.535430376677173	21.359711919528813
46	22.340936195092134	33.42565617037675	25.167509891139332	19.065897743391787
47	21.52979633056171	28.313324053988513	26.872091207765415	23.28478840768436
48	22.312367329176453	25.216259671059404	31.391038013201474	21.08033498656267
49	19.853700830822486	25.005481235902426	32.11979324113784	23.021024692137253
50	18.499586415836454	31.940490387905403	29.45749052410581	20.102432672152332
51	18.3731027449365	33.27890853644357	25.491401103555493	22.85658761506443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	834.0
1	1388.5
2	1943.0
3	4663.5
4	7384.0
5	5576.5
6	3769.0
7	3723.0
8	3677.0
9	3801.0
10	3925.0
11	3891.0
12	3857.0
13	3867.5
14	3878.0
15	3616.5
16	3355.0
17	3201.0
18	3047.0
19	3026.0
20	3005.0
21	3101.5
22	3198.0
23	4234.0
24	5270.0
25	5968.5
26	10070.0
27	13473.0
28	17160.5
29	20848.0
30	23424.5
31	26001.0
32	27703.0
33	29405.0
34	32767.5
35	36130.0
36	38986.0
37	41842.0
38	48508.5
39	55175.0
40	75415.0
41	95655.0
42	107531.5
43	119408.0
44	122674.0
45	125940.0
46	118578.0
47	111216.0
48	104900.5
49	98585.0
50	89917.0
51	81249.0
52	74519.5
53	67790.0
54	59455.5
55	51121.0
56	48221.0
57	45321.0
58	42605.0
59	39889.0
60	35864.0
61	31839.0
62	27597.0
63	23355.0
64	19133.5
65	14912.0
66	11607.0
67	8302.0
68	6811.0
69	5320.0
70	4319.0
71	3318.0
72	2537.5
73	1757.0
74	1438.5
75	866.5
76	613.0
77	526.0
78	439.0
79	307.5
80	176.0
81	110.0
82	44.0
83	36.0
84	28.0
85	20.0
86	12.0
87	10.0
88	8.0
89	5.0
90	2.0
91	3.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1204108.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.905466608355773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.45526976981785	14.584946129380846
2	7.871253571364016	2.81876935975919
3	2.3502020282958584	1.262443918216245
4	1.0532389989705981	0.7543494290674443
5	0.5971827277711547	0.5346417695596613
6	0.38635862553909406	0.4150758881064288
7	0.2778346712275037	0.3482331599815301
8	0.22355284109552695	0.32022543451512175
9	0.16862327791981846	0.2717350624966324
>10	2.370003642266267	10.943226471010444
>50	1.5555176551170546	20.818362968921754
>100	1.6807223975534877	45.014167802026805
>500	0.007447122226767046	0.9131577383921672
>1k	0.002792670835037642	1.0006648685657344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4192	0.34814152883296184	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2027	0.1683403814275796	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1858	0.15430509555621258	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1737	0.1442561630684291	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04509562265178871	0.0
2	0.0	0.0	0.0	0.14915605576908383	0.0
3	0.0	0.0	0.0	0.2165088181458806	0.0
4	0.0	0.0	0.0	0.3512973919282988	0.0
5	0.0	0.0	0.0	0.5858278493291299	0.0
6	0.0	0.0	0.0	0.7858099107389038	0.0
7	0.0	0.0	0.0	0.911878336494733	0.0
8	0.0	0.0	0.0	1.204543114072824	0.0
9	0.0	0.0	0.0	1.2835227404850729	0.0
10	0.0	0.0	0.0	1.4561816714115345	0.0
11	0.0	0.0	0.0	1.7397110558189133	0.0
12	0.0	0.0	0.0	1.9695907676055637	0.0
13	0.0	0.0	0.0	2.0634361701774258	0.0
14	0.0	0.0	0.0	2.0981506642261327	0.0
15	0.0	0.0	0.0	2.164589887285858	0.0
16	0.0	0.0	0.0	2.3187288847844214	0.0
17	0.0	0.0	0.0	2.497948688988031	0.0
18	0.0	0.0	0.0	2.7231776551605007	0.0
19	0.0	0.0	0.0	2.8391971484285463	0.0
20	0.0	0.0	0.0	2.965597770299674	0.0
21	0.0	0.0	0.0	3.1360974264766948	0.0
22	0.0	0.0	0.0	3.301281944808937	0.0
23	0.0	0.0	0.0	3.4835745630790593	0.0
24	1.6609805764931384E-4	0.0	0.0	3.616785205313809	0.0
25	1.6609805764931384E-4	0.0	0.0	3.725247236958811	0.0
26	1.6609805764931384E-4	0.0	0.0	3.8485750447634266	0.0
27	1.6609805764931384E-4	0.0	0.0	3.960109890474941	0.0
28	1.6609805764931384E-4	0.0	0.0	4.087341002634315	0.0
29	1.6609805764931384E-4	0.0	0.0	4.224039704079701	0.0
30	1.6609805764931384E-4	0.0	0.0	4.391466546190209	0.0
31	1.6609805764931384E-4	0.0	0.0	4.552664711138868	0.0
32	1.6609805764931384E-4	0.0	0.0	4.702817355253847	0.0
33	1.6609805764931384E-4	0.0	0.0	4.840678743102778	0.0
34	1.6609805764931384E-4	0.0	0.0	5.001627760964963	0.0
35	1.6609805764931384E-4	0.0	0.0	5.207589352450112	0.0
36	1.6609805764931384E-4	0.0	0.0	5.379417793088328	0.0
37	1.6609805764931384E-4	0.0	0.0	5.556312224484847	0.0
38	1.6609805764931384E-4	0.0	0.0	5.725815292315971	0.0
39	1.6609805764931384E-4	0.0	0.0	5.901630086337771	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	30	2.1655887E-6	45.000004	16
AACCGAG	30	2.1655887E-6	45.000004	1
GGTCGAC	20	7.0334197E-4	45.000004	9
ACGCATG	20	7.0334197E-4	45.000004	1
CGTTGCC	20	7.0334197E-4	45.000004	20
CACGCTA	20	7.0334197E-4	45.000004	30
CCGTGTG	20	7.0334197E-4	45.000004	13
ATTCGGT	20	7.0334197E-4	45.000004	37
CGATTAC	20	7.0334197E-4	45.000004	24
ATCGTTC	30	2.1655887E-6	45.000004	19
CCGTCCC	20	7.0334197E-4	45.000004	10
CTAACGG	80	0.0	45.000004	2
TCGGACG	20	7.0334197E-4	45.000004	27
CGTAGTG	20	7.0334197E-4	45.000004	18
TCGCTGA	20	7.0334197E-4	45.000004	15
CCGAGTT	30	2.1655887E-6	45.000004	43
TCGCTAG	20	7.0334197E-4	45.000004	1
TCCCGTA	20	7.0334197E-4	45.000004	43
TCCGTAA	20	7.0334197E-4	45.000004	32
TCGTAGC	20	7.0334197E-4	45.000004	24
>>END_MODULE