FastQCFastQC Report
Thu 26 May 2016
SRR1548104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548104_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3006556
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174320.5797996112495494No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGA207.0351065E-445.00000416
TAATGCG1450.045.0000041
CTTCGCG600.044.9999961
CGGTCTA7750.043.8387131
TCGATAG1700.043.6764681
CGACGGT7900.043.29113828
CGGGTAT1750.042.4285746
CGTTTTT77000.042.1363641
TAACGCG556.184564E-1140.9090921
TCACGAC8700.040.34482625
GGCGATA8250.040.090918
TACGGGA11700.039.8076934
TTAGGGA48750.039.5076944
CACCGTA403.4607E-739.37500425
TTGTGCG2950.038.8983041
GTAGGGT9900.038.8636364
TAGGGAT63800.038.7931025
ACGGTCT8850.038.6440730
TAGGTCG356.251659E-638.5714261
GACGGTC8950.038.46368829
Produced by FastQC (version 0.11.5)