##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548104_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3006556
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5181047683795	31.0	31.0	33.0	30.0	34.0
2	31.918231358404768	31.0	31.0	34.0	30.0	34.0
3	31.98522162900009	33.0	31.0	34.0	30.0	34.0
4	35.70318530571192	37.0	35.0	37.0	33.0	37.0
5	35.61811022312573	37.0	35.0	37.0	33.0	37.0
6	35.674318389546045	37.0	35.0	37.0	33.0	37.0
7	36.0861420841654	37.0	35.0	37.0	35.0	37.0
8	36.12868078958117	37.0	35.0	37.0	35.0	37.0
9	37.97284268112751	39.0	38.0	39.0	35.0	39.0
10	37.34057306765615	39.0	37.0	39.0	34.0	39.0
11	36.935147723840835	39.0	37.0	39.0	33.0	39.0
12	36.42293840527168	38.0	35.0	39.0	32.0	39.0
13	36.19831062518044	39.0	35.0	39.0	32.0	39.0
14	37.20486929230655	39.0	35.0	41.0	32.0	41.0
15	37.4215996642005	39.0	35.0	41.0	32.0	41.0
16	37.54176772360135	39.0	35.0	41.0	33.0	41.0
17	37.48329018318634	39.0	35.0	41.0	33.0	41.0
18	37.40743262390589	39.0	35.0	41.0	32.0	41.0
19	37.35893427562966	39.0	35.0	41.0	32.0	41.0
20	37.23558849394457	39.0	35.0	41.0	32.0	41.0
21	37.11803804752015	39.0	35.0	41.0	32.0	41.0
22	37.04237073914472	39.0	35.0	41.0	32.0	41.0
23	36.96995865036274	39.0	35.0	41.0	32.0	41.0
24	36.97735847926997	39.0	35.0	41.0	32.0	41.0
25	36.95790665465736	39.0	35.0	41.0	32.0	41.0
26	36.835228746778704	39.0	35.0	41.0	31.0	41.0
27	36.782499311504594	39.0	35.0	41.0	31.0	41.0
28	36.74133194259478	38.0	35.0	41.0	31.0	41.0
29	36.69494165417175	38.0	35.0	41.0	31.0	41.0
30	36.61753913780419	38.0	35.0	41.0	31.0	41.0
31	36.52877544938461	38.0	35.0	41.0	31.0	41.0
32	36.41840564419888	38.0	35.0	41.0	30.0	41.0
33	36.32001665693238	38.0	35.0	41.0	30.0	41.0
34	36.21089878252725	38.0	35.0	41.0	30.0	41.0
35	36.11711572975857	38.0	35.0	41.0	30.0	41.0
36	35.9643884896872	38.0	35.0	41.0	29.0	41.0
37	35.90744659337794	38.0	35.0	40.0	29.0	41.0
38	35.798102879174714	38.0	35.0	40.0	29.0	41.0
39	35.757874790956826	38.0	35.0	40.0	29.0	41.0
40	35.64319107976036	38.0	35.0	40.0	28.0	41.0
41	35.588053573590514	38.0	35.0	40.0	28.0	41.0
42	35.56019378983794	38.0	35.0	40.0	28.0	41.0
43	35.47180395109886	38.0	34.0	40.0	27.0	41.0
44	35.33066904458124	38.0	34.0	40.0	27.0	41.0
45	35.29994319081368	38.0	34.0	40.0	27.0	41.0
46	35.24233375330444	38.0	34.0	40.0	27.0	41.0
47	35.15650132576942	38.0	34.0	40.0	27.0	41.0
48	35.04565689114056	37.0	34.0	40.0	26.0	41.0
49	34.956278545950916	37.0	34.0	40.0	26.0	41.0
50	34.80287046042049	37.0	34.0	40.0	26.0	41.0
51	34.45127348368033	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	4.0
13	7.0
14	14.0
15	68.0
16	164.0
17	429.0
18	1008.0
19	1985.0
20	3539.0
21	5846.0
22	9162.0
23	13510.0
24	19870.0
25	28262.0
26	37376.0
27	44239.0
28	49762.0
29	57216.0
30	68811.0
31	84148.0
32	105320.0
33	136123.0
34	227005.0
35	354069.0
36	204440.0
37	267983.0
38	428150.0
39	857843.0
40	197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.742199380287612	24.40406897460084	31.22290088726104	12.63083075785051
2	28.678793942304747	26.258582910147027	32.03010354704852	13.03251960049971
3	28.536671194549513	25.457001299826114	32.569025822236476	13.437301683387904
4	25.0789940383615	27.361705552798615	33.17746285118255	14.381837557657331
5	23.520233782440773	31.921241447024435	30.624807919759352	13.933716850775438
6	21.808341504365792	40.7605579274093	27.074433338344605	10.356667229880303
7	91.00652041738122	2.8624113437434726	4.980416130615894	1.1506521082594172
8	92.26031379425496	1.9209687097130403	4.6297823822340245	1.1889351137979802
9	87.84033957790908	4.896133649265139	5.575648682412701	1.687878090413084
10	44.05588986202153	37.44919436059065	10.612408350285177	7.882507427102638
11	32.72175871661795	23.800388218280318	27.40800437444039	16.069848690661342
12	33.01787826336845	22.535851652189415	26.888971966595665	17.557298117846464
13	24.443881969935035	30.646127994955023	27.05048567197817	17.85950436313177
14	17.060716647220275	34.656929722912196	28.395878872703516	19.88647475716401
15	15.788862738628517	29.798314084287803	37.828265962782666	16.584557214301014
16	20.76468889985751	26.721438083973826	36.38618405910284	16.127688957065825
17	20.759899366584225	26.353941187192255	27.68859119870044	25.197568247523076
18	22.17610448632921	27.563930291004056	31.35301654118533	18.906948681481403
19	25.271174060952134	28.965035076679097	26.994541262494366	18.769249599874406
20	27.303632461860015	28.165415844574326	27.297246417495636	17.233705276070026
21	24.30106074857744	28.186769180417727	29.827018023279795	17.68515204772504
22	23.345349296670342	24.139048133478973	29.690316761104736	22.825285808745953
23	19.83475444994206	28.545950915266506	29.70638165395888	21.912912980832555
24	19.752268043568787	27.213828712985887	34.50210140772366	18.53180183572167
25	19.335778212679227	29.0179860278671	31.934978094537403	19.71125766491627
26	18.68579863471693	34.595863173677785	26.38447446180946	20.33386372979582
27	18.302236845081215	33.51605624508574	29.82325957008617	18.35844733974687
28	15.956496403193555	30.841667342966506	34.59197167789325	18.6098645759467
29	17.586367923963497	27.58348755186998	34.28384503731179	20.54629948685473
30	19.405259705789614	30.307468079756372	31.038936244660004	19.248335969794013
31	24.684156889144923	28.436057735162755	27.201755097859476	19.678030277832843
32	25.555286513871682	28.58366848979364	27.756276616833347	18.10476837950133
33	24.48219823612133	28.91354759399126	27.30017335449598	19.30408081539143
34	19.18284575441136	28.61243895008109	29.700793865140046	22.503921430367505
35	19.775783321514716	27.739380207785917	30.724855948134678	21.75998052256469
36	24.40410223524857	27.67452194471016	28.938925468210137	18.982450351831133
37	20.269304812549642	30.932701735806685	29.784045266411134	19.013948185232536
38	20.04732324959189	31.704415284465014	27.155888664638212	21.092372801304883
39	20.071769825674295	30.350706921806882	28.916175185162025	20.6613480673568
40	22.86563097444385	27.49937137375788	28.279400084349003	21.355597567449266
41	18.57713609857924	26.79461150898237	29.581221836546533	25.04703055589186
42	20.229990726931412	27.090797577028336	28.157433289118845	24.521778406921406
43	21.0360292640483	27.2936875281884	27.845082546275542	23.82520066148776
44	19.830231001850624	29.66217825312417	28.42202174182021	22.085569003204995
45	18.8764154068642	32.871797498533205	26.245345172350028	22.00644192225257
46	21.17988156548556	31.335554701126473	26.581710102855226	20.902853630532743
47	20.03208987293102	28.778309800316375	28.558889307233926	22.630711019518678
48	20.68253509996155	27.71902469137445	30.774547355845026	20.82389285281897
49	20.606634301839048	26.46396740988693	30.876491241141025	22.052907047133
50	19.37732076169544	29.960459741977196	29.43923213138222	21.22298736494514
51	18.355653445337456	31.20946358557765	27.50821870605437	22.926664263030524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1172.0
1	2792.0
2	4412.0
3	15249.5
4	26087.0
5	18306.5
6	10526.0
7	9943.0
8	9360.0
9	9833.0
10	10306.0
11	10493.5
12	10681.0
13	10507.5
14	10334.0
15	10381.5
16	10429.0
17	9732.0
18	9035.0
19	9579.0
20	10123.0
21	11687.0
22	13251.0
23	14348.5
24	15446.0
25	20049.0
26	30731.5
27	36811.0
28	47205.0
29	57599.0
30	65106.0
31	72613.0
32	79496.0
33	86379.0
34	97415.5
35	108452.0
36	116262.0
37	124072.0
38	141084.5
39	158097.0
40	185016.5
41	211936.0
42	237675.5
43	263415.0
44	272934.5
45	282454.0
46	276843.5
47	271233.0
48	263419.5
49	255606.0
50	237833.5
51	220061.0
52	199510.5
53	178960.0
54	159160.0
55	139360.0
56	125367.0
57	111374.0
58	100052.0
59	88730.0
60	77890.5
61	67051.0
62	55942.5
63	44834.0
64	36114.5
65	27395.0
66	20988.5
67	14582.0
68	11865.0
69	9148.0
70	7119.5
71	5091.0
72	3844.0
73	2597.0
74	2008.5
75	1043.5
76	667.0
77	531.5
78	396.0
79	274.0
80	152.0
81	140.0
82	128.0
83	96.5
84	65.0
85	51.5
86	38.0
87	28.0
88	18.0
89	10.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3006556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.41945605694601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9952613603569	16.130402680547732
2	8.708553513797796	3.556478515891137
3	2.9555693330523005	1.8105335435855605
4	1.3933962489260485	1.1380957389943542
5	0.810656792278339	0.8276585373596176
6	0.5285395472522584	0.6475494035685379
7	0.40492266928357806	0.5787810456328113
8	0.31549461312520277	0.5153782711130608
9	0.2655337024515693	0.48798483919631963
>10	3.071975385581626	15.204217454533584
>50	1.3094739855442992	19.551027291569596
>100	1.234379526796167	37.92588566528546
>500	0.0049289380689920715	0.6572199100259632
>1k	0.0011500855494314833	0.38396538388193724
>5k	0.0	0.0
>10k+	1.6429793563306906E-4	0.5848217188143233
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17432	0.5797996112495494	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6630323865579087E-4	0.0	0.0	0.033327169026620494	0.0
2	1.6630323865579087E-4	0.0	0.0	0.10793080188760829	0.0
3	1.6630323865579087E-4	0.0	0.0	0.16776670715596184	0.0
4	1.6630323865579087E-4	0.0	0.0	0.25939979165530264	0.0
5	1.6630323865579087E-4	0.0	0.0	0.5100852936050417	0.0
6	1.6630323865579087E-4	0.0	0.0	0.7398165874841514	0.0
7	1.6630323865579087E-4	0.0	0.0	0.8925827425133608	0.0
8	1.6630323865579087E-4	0.0	0.0	1.229247018848144	0.0
9	1.6630323865579087E-4	0.0	0.0	1.3387743318268477	0.0
10	1.6630323865579087E-4	0.0	0.0	1.568239540524108	0.0
11	1.6630323865579087E-4	0.0	0.0	1.9790085400039115	0.0
12	1.6630323865579087E-4	0.0	0.0	2.289662989812929	0.0
13	1.6630323865579087E-4	0.0	0.0	2.4108647901452693	0.0
14	1.6630323865579087E-4	0.0	0.0	2.4609553256283934	3.3260647731158176E-5
15	1.6630323865579087E-4	0.0	0.0	2.536989166341821	3.3260647731158176E-5
16	1.6630323865579087E-4	0.0	0.0	2.708547587339135	3.3260647731158176E-5
17	1.6630323865579087E-4	0.0	0.0	2.948456639423979	3.3260647731158176E-5
18	1.6630323865579087E-4	0.0	0.0	3.2091868569885276	3.3260647731158176E-5
19	1.6630323865579087E-4	0.0	0.0	3.4011340550450417	3.3260647731158176E-5
20	1.6630323865579087E-4	0.0	0.0	3.585497825418851	3.3260647731158176E-5
21	1.6630323865579087E-4	0.0	0.0	3.837846359755148	3.3260647731158176E-5
22	1.6630323865579087E-4	0.0	0.0	4.129010069993707	3.3260647731158176E-5
23	1.6630323865579087E-4	0.0	0.0	4.428821548642367	3.3260647731158176E-5
24	1.6630323865579087E-4	0.0	0.0	4.643485769099262	3.3260647731158176E-5
25	1.6630323865579087E-4	0.0	0.0	4.831874077848542	3.3260647731158176E-5
26	1.6630323865579087E-4	0.0	0.0	5.018433050972608	3.3260647731158176E-5
27	1.6630323865579087E-4	0.0	0.0	5.209049823119876	3.3260647731158176E-5
28	1.6630323865579087E-4	0.0	0.0	5.410409784484307	3.3260647731158176E-5
29	1.6630323865579087E-4	0.0	0.0	5.633322645578529	3.3260647731158176E-5
30	1.6630323865579087E-4	0.0	0.0	5.907689728712853	3.3260647731158176E-5
31	1.9956388638694904E-4	0.0	0.0	6.152421574718715	3.3260647731158176E-5
32	1.9956388638694904E-4	0.0	0.0	6.376265733949409	3.3260647731158176E-5
33	1.9956388638694904E-4	0.0	0.0	6.60230509593036	3.3260647731158176E-5
34	1.9956388638694904E-4	0.0	0.0	6.844442611413192	3.3260647731158176E-5
35	1.9956388638694904E-4	0.0	0.0	7.133810246674268	3.3260647731158176E-5
36	1.9956388638694904E-4	0.0	0.0	7.384695312510394	3.3260647731158176E-5
37	1.9956388638694904E-4	0.0	0.0	7.644294668052083	3.3260647731158176E-5
38	1.9956388638694904E-4	0.0	0.0	7.93309687230173	3.3260647731158176E-5
39	1.9956388638694904E-4	0.0	0.0	8.353079071203064	3.3260647731158176E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTCGA	20	7.0351065E-4	45.000004	16
TAATGCG	145	0.0	45.000004	1
CTTCGCG	60	0.0	44.999996	1
CGGTCTA	775	0.0	43.83871	31
TCGATAG	170	0.0	43.676468	1
CGACGGT	790	0.0	43.291138	28
CGGGTAT	175	0.0	42.428574	6
CGTTTTT	7700	0.0	42.136364	1
TAACGCG	55	6.184564E-11	40.909092	1
TCACGAC	870	0.0	40.344826	25
GGCGATA	825	0.0	40.09091	8
TACGGGA	1170	0.0	39.807693	4
TTAGGGA	4875	0.0	39.507694	4
CACCGTA	40	3.4607E-7	39.375004	25
TTGTGCG	295	0.0	38.898304	1
GTAGGGT	990	0.0	38.863636	4
TAGGGAT	6380	0.0	38.793102	5
ACGGTCT	885	0.0	38.64407	30
TAGGTCG	35	6.251659E-6	38.571426	1
GACGGTC	895	0.0	38.463688	29
>>END_MODULE