FastQCFastQC Report
Thu 26 May 2016
SRR1548103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548103_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2132015
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73880.34652664263619154No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28670.1344737255600922No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28120.13189400637425158No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA207.034645E-445.034
CGATCTT207.034645E-445.010
TATCGCC207.034645E-445.041
TCGGTTA207.034645E-445.036
CCGTTCG253.8919323E-545.045
TACGTAA253.8919323E-545.028
GTCAACG302.16638E-644.9999961
CTCGCTA302.16638E-644.99999621
CGATACG600.044.99999610
CTACGCG750.042.01
CGTTTTT42000.040.928571
GGCGATA4900.040.8673488
ACGACGG5550.040.5405427
TATCGCG1000.040.51
GGGCGAT21300.040.246487
TTACGAG1400.040.1785741
CGGTCTA5600.040.17857431
CGTAAGG2650.039.9056632
TACGGGA8800.039.8863644
ACGGGAC7400.039.831085