##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548103_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2132015
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.537128022082396	31.0	31.0	33.0	30.0	34.0
2	31.972693437897952	31.0	31.0	34.0	30.0	34.0
3	32.061356041116035	33.0	31.0	34.0	30.0	34.0
4	35.75929672164595	37.0	35.0	37.0	35.0	37.0
5	35.65317035761944	37.0	35.0	37.0	33.0	37.0
6	35.70607617676236	37.0	35.0	37.0	33.0	37.0
7	36.06717963991811	37.0	35.0	37.0	35.0	37.0
8	36.09400684329144	37.0	35.0	37.0	35.0	37.0
9	37.88427379732319	39.0	38.0	39.0	35.0	39.0
10	37.34371709392288	39.0	37.0	39.0	34.0	39.0
11	37.03889372260514	39.0	37.0	39.0	33.0	39.0
12	36.47893893804687	39.0	35.0	39.0	32.0	39.0
13	36.26983065316145	39.0	35.0	39.0	32.0	39.0
14	37.31730311465914	40.0	35.0	41.0	32.0	41.0
15	37.516144117184915	40.0	35.0	41.0	32.0	41.0
16	37.62743554806134	40.0	35.0	41.0	33.0	41.0
17	37.572107138082984	39.0	35.0	41.0	33.0	41.0
18	37.476941297317325	39.0	36.0	41.0	32.0	41.0
19	37.44052222897118	39.0	36.0	41.0	32.0	41.0
20	37.32445737952125	39.0	35.0	41.0	32.0	41.0
21	37.18287629308424	39.0	35.0	41.0	32.0	41.0
22	37.13776450916152	39.0	35.0	41.0	32.0	41.0
23	37.05163237594482	39.0	35.0	41.0	32.0	41.0
24	37.06490338951649	39.0	35.0	41.0	32.0	41.0
25	37.05066521577006	39.0	35.0	41.0	32.0	41.0
26	36.91616428589855	39.0	35.0	41.0	31.0	41.0
27	36.856522585441475	39.0	35.0	41.0	31.0	41.0
28	36.80296526994416	39.0	35.0	41.0	31.0	41.0
29	36.75502142339524	39.0	35.0	41.0	31.0	41.0
30	36.66403894906931	38.0	35.0	41.0	31.0	41.0
31	36.6125167974897	39.0	35.0	41.0	31.0	41.0
32	36.50458416099324	39.0	35.0	41.0	30.0	41.0
33	36.41628506366043	39.0	35.0	41.0	30.0	41.0
34	36.29048107072418	39.0	35.0	41.0	30.0	41.0
35	36.18099966463651	38.0	35.0	41.0	30.0	41.0
36	36.03801849424136	38.0	35.0	41.0	29.0	41.0
37	35.97306913881938	38.0	35.0	41.0	29.0	41.0
38	35.87076732574583	38.0	35.0	41.0	29.0	41.0
39	35.82329205000903	38.0	35.0	40.0	29.0	41.0
40	35.69423526569935	38.0	35.0	40.0	28.0	41.0
41	35.626696341254636	38.0	35.0	40.0	28.0	41.0
42	35.57422672917404	38.0	35.0	40.0	27.0	41.0
43	35.508169970661555	38.0	34.0	40.0	27.0	41.0
44	35.3462921227102	38.0	34.0	40.0	27.0	41.0
45	35.32266611632657	38.0	34.0	40.0	27.0	41.0
46	35.24810332009859	38.0	34.0	40.0	27.0	41.0
47	35.185540439443436	38.0	34.0	40.0	27.0	41.0
48	35.070520610783696	38.0	34.0	40.0	26.0	41.0
49	34.97565120320448	37.0	34.0	40.0	26.0	41.0
50	34.82812409856403	37.0	34.0	40.0	26.0	41.0
51	34.46479410323098	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	6.0
12	7.0
13	11.0
14	27.0
15	67.0
16	177.0
17	373.0
18	881.0
19	1639.0
20	2809.0
21	4520.0
22	6679.0
23	10170.0
24	14164.0
25	19863.0
26	26283.0
27	31369.0
28	35566.0
29	40256.0
30	48348.0
31	58173.0
32	71843.0
33	93442.0
34	155083.0
35	229684.0
36	144124.0
37	195905.0
38	316839.0
39	623594.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.59632085140114	25.33209194119178	31.044950434213643	14.026636773193434
2	30.588762274186625	26.019188420344136	29.786375799419794	13.605673506049442
3	29.598947474572178	25.97960145683778	30.019629317804984	14.401821750785054
4	27.31885094617064	27.274057640307408	30.242563959446816	15.164527454075136
5	23.780742630797626	31.1540960077673	29.734593799762198	15.330567561672877
6	22.56410015876999	39.88325598084441	26.828704300860924	10.723939559524675
7	88.93731985938184	3.635715508568185	5.568534930570376	1.8584297014795863
8	89.70518500104362	2.8731974212188938	5.484811316993548	1.936806260743944
9	84.06150988618748	6.272563748378881	7.058533828326724	2.6073925371069153
10	41.020067870066576	37.52792546018672	12.364359537808129	9.087647131938565
11	34.36124980358956	21.702802278595602	27.56823943546363	16.367708482351205
12	31.8323276337174	22.311662910439185	28.796420287849756	17.059589167993657
13	23.756164942554346	29.20026360039681	29.196276761655053	17.847294695393796
14	18.498462721885165	33.56327230343126	29.832060281001777	18.1062046936818
15	17.147393428282633	27.922505235657347	38.85413564163479	16.075965694425225
16	21.948719872984007	25.776366488978734	35.8610985382373	16.413815099799955
17	21.629444445747335	24.736739657084964	28.925077919245407	24.708737977922294
18	22.919726174534418	27.330014094647552	31.262866349439378	18.48739338137865
19	26.18888703878725	27.856183000588647	27.507686390574175	18.447243570049928
20	27.53038791940957	27.946144844196684	26.868947920160036	17.654519316233706
21	24.676327324151096	26.914491689786423	30.30349223621785	18.10568874984463
22	24.1745015865273	23.48365278855918	30.16845566283539	22.173389962078126
23	20.980152578663848	27.385454605150528	30.287779401176824	21.346613415008804
24	20.55684411226	26.82518650197114	33.47968940181003	19.138279983958835
25	20.7591410004151	27.943940356892423	31.53383067192304	19.763087970769437
26	20.45215441730007	32.496394256137975	26.82617148566028	20.225279840901685
27	19.06590713479971	31.2917123003356	31.276984448983708	18.365396115880987
28	17.78069103641391	28.862648714948065	34.46293764349688	18.893722605141143
29	18.90141485871347	27.28503317284353	34.129121980849106	19.684429987593894
30	20.28100177531584	27.928508945762577	32.64118685844143	19.149302420480154
31	25.294521849048905	26.888882113868807	27.805667408531363	20.010928628550925
32	25.27561954301447	27.995440932638843	28.60265992500053	18.12627959934616
33	24.215777093500748	27.513596292709007	28.397595701718796	19.873030912071442
34	20.182315790461136	27.712093958063143	30.709211708172784	21.396378543302934
35	20.671383644111323	27.338738235894212	30.426755909315833	21.56312221067863
36	24.692040159192125	26.66106945776648	29.409127046479504	19.23776333656189
37	20.98836077607334	29.066774858525857	29.837313527343852	20.107550838056955
38	20.35529768786805	30.399410885945926	27.586109853823732	21.659181572362296
39	20.269275779016564	28.38314927427809	29.734030951939832	21.613543994765514
40	22.29960858624353	27.360501685025667	28.53474295443512	21.80514677429568
41	19.229789659078385	25.844283459544144	30.118596726570875	24.807330154806603
42	21.402945101230525	26.92959477301989	28.649939142079205	23.017520983670376
43	21.94614015379817	26.872278103109032	28.221893373170452	22.95968836992235
44	20.687987654871094	28.570952831007286	29.946881236764284	20.794178277357336
45	20.200983576569584	30.55456926897794	27.549853073266373	21.694594081186107
46	21.840746899060278	29.55054256184877	28.014671566569653	20.5940389725213
47	21.09933560504968	28.24117091108646	29.236004437116996	21.42348904674686
48	21.436997394483623	26.9893035461758	30.72454931133224	20.84914974800834
49	21.0861086812241	25.92622472168348	31.40742443181685	21.580242165275575
50	19.987617347907964	29.096793409052	29.807998536595665	21.107590706444373
51	18.915157726376222	30.003119115015608	28.880988173160134	22.20073498544804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1119.0
1	2535.0
2	3951.0
3	8769.0
4	13587.0
5	10297.5
6	7008.0
7	6743.0
8	6478.0
9	6330.0
10	6182.0
11	6421.5
12	6661.0
13	6431.5
14	6202.0
15	5940.0
16	5678.0
17	6070.5
18	6463.0
19	6409.0
20	6355.0
21	8762.5
22	11170.0
23	12954.5
24	14739.0
25	17799.0
26	25963.0
27	31067.0
28	37312.5
29	43558.0
30	47598.0
31	51638.0
32	57403.0
33	63168.0
34	71805.5
35	80443.0
36	87544.0
37	94645.0
38	102314.5
39	109984.0
40	127154.0
41	144324.0
42	158012.5
43	171701.0
44	173455.5
45	175210.0
46	176166.5
47	177123.0
48	174584.0
49	172045.0
50	161519.0
51	150993.0
52	138878.0
53	126763.0
54	113437.0
55	100111.0
56	94490.5
57	88870.0
58	79277.5
59	69685.0
60	62641.5
61	55598.0
62	47398.0
63	39198.0
64	32192.0
65	25186.0
66	21218.5
67	17251.0
68	14329.0
69	11407.0
70	9113.5
71	6820.0
72	5528.0
73	4236.0
74	3175.5
75	1701.0
76	1287.0
77	966.0
78	645.0
79	453.0
80	261.0
81	170.5
82	80.0
83	77.5
84	75.0
85	48.0
86	21.0
87	20.0
88	19.0
89	18.0
90	17.0
91	12.5
92	8.0
93	8.5
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2132015.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.455862810942985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52294046825548	15.860991456950508
2	7.162899140226497	2.787207660017364
3	2.1794699377602456	1.2721040432891337
4	1.0645471606311525	0.8284673405207437
5	0.6024936929024861	0.5861017316784592
6	0.45358817006260255	0.5294969525642803
7	0.3438144801954954	0.46824451513694626
8	0.2613598709752182	0.4067985435183684
9	0.23152737697938	0.4054108395139467
>10	3.1229118646402756	15.44027866260477
>50	1.7737117168574241	25.264191231861087
>100	1.27662421985089	35.03154603463429
>500	0.00266064160533325	0.32348467872844267
>1k	0.0012093825478787501	0.4480032210495744
>5k	2.4187650957575E-4	0.3476730879321697
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7388	0.34652664263619154	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2867	0.1344737255600922	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2812	0.13189400637425158	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.814239111826136E-4	0.0	0.0	0.015431411129846648	0.0
2	2.814239111826136E-4	0.0	0.0	0.0700276498992737	0.0
3	2.814239111826136E-4	0.0	0.0	0.11796352277071222	0.0
4	2.814239111826136E-4	0.0	0.0	0.20891035006789352	0.0
5	2.814239111826136E-4	0.0	0.0	0.3909447166178474	0.0
6	2.814239111826136E-4	0.0	0.0	0.5540767771333691	0.0
7	3.283278963797159E-4	0.0	0.0	0.6552017692183216	0.0
8	3.283278963797159E-4	0.0	0.0	0.8785585467269227	0.0
9	3.283278963797159E-4	0.0	0.0	0.9522916114567674	0.0
10	3.752318815768182E-4	0.0	0.0	1.0944575905891845	0.0
11	3.752318815768182E-4	0.0	0.0	1.3435177519857975	0.0
12	3.752318815768182E-4	0.0	0.0	1.5470341437560242	0.0
13	3.752318815768182E-4	0.0	0.0	1.6280373261914198	0.0
14	3.752318815768182E-4	0.0	0.0	1.656930181072835	0.0
15	3.752318815768182E-4	0.0	0.0	1.707445773130114	0.0
16	3.752318815768182E-4	0.0	0.0	1.8334298773695308	0.0
17	3.752318815768182E-4	0.0	0.0	1.9771905919986492	0.0
18	3.752318815768182E-4	0.0	0.0	2.153737192280542	0.0
19	3.752318815768182E-4	0.0	0.0	2.2627420538786076	0.0
20	3.752318815768182E-4	0.0	0.0	2.3901332776739377	0.0
21	3.752318815768182E-4	0.0	0.0	2.547730667936201	0.0
22	3.752318815768182E-4	0.0	0.0	2.7174761903645144	0.0
23	3.752318815768182E-4	0.0	0.0	2.887972176555981	0.0
24	3.752318815768182E-4	0.0	0.0	3.0161138641144647	0.0
25	3.752318815768182E-4	0.0	0.0	3.1318259955957157	0.0
26	3.752318815768182E-4	0.0	0.0	3.240080393430628	0.0
27	3.752318815768182E-4	0.0	0.0	3.3647511860845256	0.0
28	3.752318815768182E-4	0.0	0.0	3.4940185692877397	0.0
29	3.752318815768182E-4	0.0	0.0	3.646128193281942	0.0
30	3.752318815768182E-4	0.0	0.0	3.817656067147745	0.0
31	3.752318815768182E-4	0.0	0.0	3.983602366775093	0.0
32	3.752318815768182E-4	0.0	0.0	4.1322410958647104	0.0
33	3.752318815768182E-4	0.0	0.0	4.286273783251994	0.0
34	3.752318815768182E-4	0.0	0.0	4.447811108270814	0.0
35	3.752318815768182E-4	0.0	0.0	4.634582777325676	0.0
36	3.752318815768182E-4	0.0	0.0	4.805782323295099	0.0
37	4.2213586677392046E-4	0.0	0.0	4.976841157308931	0.0
38	4.2213586677392046E-4	0.0	0.0	5.1876745707699055	0.0
39	4.2213586677392046E-4	0.0	0.0	5.501086999856943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	20	7.034645E-4	45.0	34
CGATCTT	20	7.034645E-4	45.0	10
TATCGCC	20	7.034645E-4	45.0	41
TCGGTTA	20	7.034645E-4	45.0	36
CCGTTCG	25	3.8919323E-5	45.0	45
TACGTAA	25	3.8919323E-5	45.0	28
GTCAACG	30	2.16638E-6	44.999996	1
CTCGCTA	30	2.16638E-6	44.999996	21
CGATACG	60	0.0	44.999996	10
CTACGCG	75	0.0	42.0	1
CGTTTTT	4200	0.0	40.92857	1
GGCGATA	490	0.0	40.867348	8
ACGACGG	555	0.0	40.54054	27
TATCGCG	100	0.0	40.5	1
GGGCGAT	2130	0.0	40.24648	7
TTACGAG	140	0.0	40.178574	1
CGGTCTA	560	0.0	40.178574	31
CGTAAGG	265	0.0	39.905663	2
TACGGGA	880	0.0	39.886364	4
ACGGGAC	740	0.0	39.83108	5
>>END_MODULE