FastQCFastQC Report
Thu 26 May 2016
SRR1548102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548102_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2189669
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86150.39343846033350244No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31990.1460951404070661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT351.2126111E-745.00000421
GTCGGAC302.1664055E-645.00000420
CGGACCG351.2126111E-745.0000046
CGAAACG207.0346886E-445.032
CGCATCG950.045.021
TAGCGCC207.0346886E-445.020
CGTTCGA253.891968E-545.026
CGCGTGA207.0346886E-445.09
TCGAATC253.891968E-545.013
CCGTTCG207.0346886E-445.035
ACGACTC207.0346886E-445.023
TACGTCG207.0346886E-445.026
CGACGGT5700.044.21052628
CGGTCTA5850.043.84615331
TCACGAC5950.043.48739625
CACGACG5950.043.1092426
TTACGGG6500.042.5769233
GGCGATA4150.041.7469868
CTCACGA6200.041.7338724
GACGGTC6150.041.70731729
Produced by FastQC (version 0.11.5)