##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548102_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2189669
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46715736488026	31.0	31.0	33.0	30.0	34.0
2	31.869928285964683	31.0	31.0	34.0	30.0	34.0
3	31.96488556032898	33.0	31.0	34.0	30.0	34.0
4	35.678028048988224	37.0	35.0	37.0	33.0	37.0
5	35.551880672375596	37.0	35.0	37.0	33.0	37.0
6	35.605838599349944	37.0	35.0	37.0	33.0	37.0
7	36.044350995515764	37.0	35.0	37.0	35.0	37.0
8	36.09273821751141	37.0	35.0	37.0	35.0	37.0
9	37.927700031374606	39.0	38.0	39.0	35.0	39.0
10	37.289372960022725	39.0	37.0	39.0	34.0	39.0
11	36.8558704534795	39.0	37.0	39.0	32.0	39.0
12	36.21223116370557	38.0	35.0	39.0	32.0	39.0
13	35.91063626511587	38.0	35.0	39.0	31.0	39.0
14	36.85540188950933	39.0	35.0	41.0	31.0	41.0
15	37.14252245430702	39.0	35.0	41.0	32.0	41.0
16	37.28626381430253	39.0	35.0	41.0	32.0	41.0
17	37.24135611364092	39.0	35.0	41.0	32.0	41.0
18	37.20104865164552	39.0	35.0	41.0	32.0	41.0
19	37.1660940534848	39.0	35.0	41.0	32.0	41.0
20	37.04003984163816	39.0	35.0	41.0	32.0	41.0
21	36.901448575104276	39.0	35.0	41.0	32.0	41.0
22	36.83113338134668	38.0	35.0	41.0	31.0	41.0
23	36.77231033548906	38.0	35.0	41.0	31.0	41.0
24	36.78796429962702	38.0	35.0	41.0	31.0	41.0
25	36.785177577067586	38.0	35.0	41.0	31.0	41.0
26	36.66355097505605	38.0	35.0	41.0	31.0	41.0
27	36.596335336528035	38.0	35.0	41.0	31.0	41.0
28	36.582852476789874	38.0	35.0	40.0	31.0	41.0
29	36.550859056779814	38.0	35.0	40.0	31.0	41.0
30	36.470312179603404	38.0	35.0	40.0	31.0	41.0
31	36.3819335251127	38.0	35.0	40.0	30.0	41.0
32	36.283594004390615	38.0	35.0	40.0	30.0	41.0
33	36.20716829803957	38.0	35.0	41.0	30.0	41.0
34	36.12504675364176	38.0	35.0	40.0	30.0	41.0
35	36.03914472918053	38.0	35.0	40.0	30.0	41.0
36	35.88472367284736	38.0	35.0	40.0	30.0	41.0
37	35.812013596575554	38.0	35.0	40.0	29.0	41.0
38	35.70545822222446	38.0	35.0	40.0	29.0	41.0
39	35.67917479765207	38.0	35.0	40.0	29.0	41.0
40	35.545112526139796	38.0	34.0	40.0	28.0	41.0
41	35.48378407878086	38.0	34.0	40.0	28.0	41.0
42	35.44179736754733	38.0	34.0	40.0	27.0	41.0
43	35.35401743368518	38.0	34.0	40.0	27.0	41.0
44	35.20627227220187	38.0	34.0	40.0	27.0	41.0
45	35.17670159279781	37.0	34.0	40.0	27.0	41.0
46	35.08036785468489	37.0	34.0	40.0	27.0	41.0
47	35.00871912604143	37.0	34.0	40.0	27.0	41.0
48	34.9188429849443	37.0	34.0	40.0	26.0	41.0
49	34.832768331651955	37.0	34.0	40.0	26.0	41.0
50	34.67214953492971	37.0	34.0	40.0	25.0	41.0
51	34.3038313096637	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	7.0
14	15.0
15	46.0
16	118.0
17	291.0
18	678.0
19	1467.0
20	2674.0
21	4147.0
22	6702.0
23	9843.0
24	14504.0
25	20520.0
26	27544.0
27	32672.0
28	37431.0
29	43771.0
30	53388.0
31	65399.0
32	81340.0
33	105116.0
34	178834.0
35	281588.0
36	145322.0
37	186886.0
38	296361.0
39	592901.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.742591688515482	25.286013548166412	32.16814961530715	11.803245148010955
2	28.371274379826357	26.441941681596624	31.651724530054544	13.535059408522477
3	28.406348174084762	26.177472485567456	31.593679227317008	13.822500113030783
4	27.00188932665165	27.194795195072864	31.421644093239664	14.38167138503582
5	23.099153342354484	32.28433155878811	30.367101146337642	14.249413952519765
6	21.913175004989338	41.26167014283894	26.350969027738895	10.474185824432826
7	90.98713093166137	3.022420283613642	4.624214892753197	1.3662338919718002
8	92.24220647047568	1.9660962455969373	4.2692297328957025	1.5224675510316856
9	87.83076346242285	4.633531369353085	5.605870110961977	1.9298350572620793
10	46.877587434447854	34.448037580109144	10.503413986314825	8.170960999128178
11	34.56431086159598	22.905608107892107	26.53775524976606	15.992325780745857
12	35.004057690911274	22.42809301314491	26.037633998563255	16.53021529738056
13	22.992835903508706	33.10605392869881	26.423674080420373	17.47743608737211
14	15.47320622431975	36.22693658265245	28.90318125707584	19.39667593595196
15	14.706103981926036	28.947251844913545	40.339841318482385	16.006802854678035
16	19.139833463413876	26.387184547070813	37.64591817302067	16.82706381649464
17	19.43275444827506	25.89478135736497	27.404826939596806	27.267637254763162
18	21.004955543509087	27.48246424459587	32.65201270146309	18.86056751043194
19	25.367578387418373	29.510990017212645	26.173727627326322	18.94770396804266
20	27.588964359453417	28.260024688662988	26.797246524474705	17.35376442740889
21	22.727910017450125	29.31890619084437	30.573753384643982	17.379430407061523
22	22.85774699281033	24.37477993249208	29.100882370805813	23.666590703891778
23	18.84513138743801	29.79806536969743	29.360053962493875	21.996749280370686
24	19.122159559275854	27.133827076147128	36.210495741593824	17.5335176229832
25	18.297149021153423	28.94469438074887	32.77340091127928	19.984755686818417
26	17.117153323173504	35.85642396179514	26.260681408925272	20.765741306106083
27	16.91091210589363	34.74529712024968	30.25735853227132	18.08643224158537
28	14.859962852833009	30.363995654137682	35.81340376102507	18.962637732004243
29	16.311232428280256	27.355047726391525	35.26491903570814	21.068800809620083
30	18.442330781501678	31.49202002677117	31.089584772858363	18.976064418868788
31	24.679711865126645	28.594824149220727	26.6715654283821	20.05389855727053
32	25.771201035407636	28.48279808500737	27.28247968071887	18.463521198866133
33	24.36596581492454	28.77051280353332	26.788569413915987	20.074951967626156
34	18.000894199077578	29.314339290550308	29.89049943164926	22.794267078722857
35	18.511245306939088	26.892055374579442	31.761330137112047	22.835369181369423
36	25.108178450715613	27.244985429304613	28.52449388469216	19.12234223528762
37	18.524946007821274	31.711002895871477	30.39518758314613	19.368863513161124
38	19.147277510893197	32.433166839371616	27.490501989113426	20.929053660621765
39	18.33825112379999	30.90563002901352	29.74276020713633	21.01335864005016
40	22.907937227042076	27.828452610874066	27.552474826103857	21.711135335980003
41	17.924352950149085	25.902727763876644	29.355121710176284	26.817797575797986
42	19.740472190089005	26.659463142602828	27.21511790138144	26.38494676592672
43	19.844506178787753	27.105740639338638	28.365154733432313	24.684598448441296
44	18.94080794859862	29.21048797786332	29.679234624045918	22.169469449492137
45	18.136942158837705	33.92266137028017	25.651548247703193	22.288848223178938
46	20.50424059526805	32.148740289057386	27.49228308022811	19.85473603544645
47	19.013193318259518	28.530293848065618	29.42645669276955	23.030056140905312
48	19.57049216114399	26.603107592974094	32.56190775866124	21.26449248722067
49	19.512857879432918	26.071794412762845	32.38905058253096	22.026297125273274
50	18.45265197616626	30.243475155377364	30.230779172559867	21.073093695896503
51	17.349014851103068	31.83768871002878	27.387792401499954	23.425504037368206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	742.0
1	1781.5
2	2821.0
3	7943.0
4	13065.0
5	9369.5
6	5674.0
7	5579.5
8	5485.0
9	5891.0
10	6297.0
11	6543.0
12	6789.0
13	6661.5
14	6534.0
15	6153.5
16	5773.0
17	5752.5
18	5732.0
19	5751.5
20	5771.0
21	6518.5
22	7266.0
23	8968.5
24	10671.0
25	14501.5
26	23574.5
27	28817.0
28	37391.5
29	45966.0
30	50193.5
31	54421.0
32	63875.5
33	73330.0
34	80879.5
35	88429.0
36	93178.0
37	97927.0
38	107760.5
39	117594.0
40	138467.0
41	159340.0
42	182586.0
43	205832.0
44	208827.5
45	211823.0
46	210941.5
47	210060.0
48	201589.5
49	193119.0
50	177510.5
51	161902.0
52	144636.0
53	127370.0
54	109350.5
55	91331.0
56	82017.0
57	72703.0
58	64046.0
59	55389.0
60	46976.0
61	38563.0
62	31370.5
63	24178.0
64	19677.5
65	15177.0
66	11175.5
67	7174.0
68	5682.5
69	4191.0
70	3126.5
71	2062.0
72	1512.0
73	962.0
74	700.0
75	365.5
76	293.0
77	225.0
78	157.0
79	112.0
80	67.0
81	52.0
82	37.0
83	30.0
84	23.0
85	18.0
86	13.0
87	15.0
88	17.0
89	11.5
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2189669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.487443978918026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95924373691471	16.966323264146894
2	9.21427647500563	3.959824991258915
3	3.101047751953366	1.9990076953814293
4	1.4497714207394314	1.2460752874149972
5	0.8525038309706647	0.9159064154897583
6	0.5391151225485608	0.6950523596369838
7	0.40619365043520317	0.6109644315823048
8	0.31224358465118524	0.5367453226375113
9	0.23812720208542434	0.46050704232003437
>10	2.6200188007076646	13.468759964110586
>50	1.0669502602147958	16.727657103711735
>100	1.2356015967814007	41.14655427549545
>500	0.004053250993401799	0.6086954076373906
>1k	6.399869989581788E-4	0.26302389103270934
>5k	2.1332899965272625E-4	0.39490254814339326
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8615	0.39343846033350244	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3199	0.1460951404070661	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.13380058812542E-5	0.0	0.0	0.06978223649327821	0.0
2	9.13380058812542E-5	0.0	0.0	0.2545133533881148	0.0
3	9.13380058812542E-5	0.0	0.0	0.3773173022954611	0.0
4	9.13380058812542E-5	0.0	0.0	0.564468876346151	0.0
5	9.13380058812542E-5	0.0	0.0	1.0250407710023752	0.0
6	9.13380058812542E-5	0.0	0.0	1.3795235718275227	0.0
7	9.13380058812542E-5	0.0	0.0	1.6307487570039125	0.0
8	9.13380058812542E-5	0.0	0.0	2.1772240461914563	0.0
9	9.13380058812542E-5	0.0	0.0	2.346519040092361	0.0
10	9.13380058812542E-5	0.0	0.0	2.706071100243918	0.0
11	9.13380058812542E-5	0.0	0.0	3.2978957093515047	0.0
12	9.13380058812542E-5	0.0	0.0	3.7201969795434837	0.0
13	9.13380058812542E-5	0.0	0.0	3.8889896144120413	0.0
14	9.13380058812542E-5	0.0	0.0	3.9574017808171007	0.0
15	9.13380058812542E-5	0.0	0.0	4.069245169018696	0.0
16	9.13380058812542E-5	0.0	0.0	4.323986867421515	0.0
17	9.13380058812542E-5	0.0	0.0	4.653260378623436	0.0
18	9.13380058812542E-5	0.0	0.0	5.006829799389771	0.0
19	9.13380058812542E-5	0.0	0.0	5.2498802330397885	0.0
20	9.13380058812542E-5	0.0	0.0	5.490921230560418	0.0
21	9.13380058812542E-5	0.0	0.0	5.813938088359474	0.0
22	9.13380058812542E-5	0.0	0.0	6.175956274669825	0.0
23	9.13380058812542E-5	0.0	0.0	6.545007487433032	0.0
24	9.13380058812542E-5	0.0	0.0	6.817057737950348	0.0
25	9.13380058812542E-5	0.0	0.0	7.0553129262916	0.0
26	9.13380058812542E-5	0.0	0.0	7.269637557091962	0.0
27	9.13380058812542E-5	0.0	0.0	7.514332074847842	0.0
28	9.13380058812542E-5	0.0	0.0	7.755692755389057	0.0
29	9.13380058812542E-5	0.0	0.0	8.029387090012234	0.0
30	9.13380058812542E-5	0.0	0.0	8.365236937637606	0.0
31	9.13380058812542E-5	0.0	0.0	8.656285493378222	0.0
32	9.13380058812542E-5	0.0	0.0	8.920297999377988	0.0
33	9.13380058812542E-5	0.0	0.0	9.191937228868838	0.0
34	9.13380058812542E-5	0.0	0.0	9.475633075136013	0.0
35	9.13380058812542E-5	0.0	0.0	9.808605775576126	0.0
36	9.13380058812542E-5	0.0	0.0	10.089926833690388	0.0
37	9.13380058812542E-5	0.0	0.0	10.3849029236839	0.0
38	9.13380058812542E-5	0.0	0.0	10.715729180985802	0.0
39	9.13380058812542E-5	0.0	0.0	11.19187420564478	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	35	1.2126111E-7	45.000004	21
GTCGGAC	30	2.1664055E-6	45.000004	20
CGGACCG	35	1.2126111E-7	45.000004	6
CGAAACG	20	7.0346886E-4	45.0	32
CGCATCG	95	0.0	45.0	21
TAGCGCC	20	7.0346886E-4	45.0	20
CGTTCGA	25	3.891968E-5	45.0	26
CGCGTGA	20	7.0346886E-4	45.0	9
TCGAATC	25	3.891968E-5	45.0	13
CCGTTCG	20	7.0346886E-4	45.0	35
ACGACTC	20	7.0346886E-4	45.0	23
TACGTCG	20	7.0346886E-4	45.0	26
CGACGGT	570	0.0	44.210526	28
CGGTCTA	585	0.0	43.846153	31
TCACGAC	595	0.0	43.487396	25
CACGACG	595	0.0	43.10924	26
TTACGGG	650	0.0	42.576923	3
GGCGATA	415	0.0	41.746986	8
CTCACGA	620	0.0	41.73387	24
GACGGTC	615	0.0	41.707317	29
>>END_MODULE