FastQCFastQC Report
Thu 26 May 2016
SRR1548101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548101_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2305320
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93640.4061908975760415No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCA207.0347654E-445.035
TGCGGTA207.0347654E-445.015
CGCGTAG207.0347654E-445.036
TAGGACG750.045.01
CGCATCG600.044.99999621
CGACGGT5350.042.89719428
TCGTTAG1350.041.6666681
TATAGCG1900.041.4473721
CGTTTTT36700.041.2602161
CGGGGTA2900.040.3448266
GACCGAT6050.040.1652879
TACGGGA10550.039.668254
CGAGGGA18900.039.2857134
TTTACGG2350.039.2553182
TAGGGAC28550.039.0893145
TATGGGA29500.038.8983084
GGGCGAT23350.038.8329777
AGGGATT53200.038.8251886
ACGTCGA356.2510244E-638.57142638
TCGTCCC1700.038.3823538
Produced by FastQC (version 0.11.5)