##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548101_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2305320
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58317804035882	31.0	31.0	33.0	30.0	34.0
2	32.004598493918415	31.0	31.0	34.0	30.0	34.0
3	32.09461376294831	33.0	31.0	34.0	30.0	34.0
4	35.78899632155189	37.0	35.0	37.0	35.0	37.0
5	35.68113537383096	37.0	35.0	37.0	33.0	37.0
6	35.74489788836257	37.0	35.0	37.0	33.0	37.0
7	36.11782919507921	37.0	35.0	37.0	35.0	37.0
8	36.14999219197335	37.0	35.0	37.0	35.0	37.0
9	37.97025705758853	39.0	38.0	39.0	35.0	39.0
10	37.403665434733576	39.0	37.0	39.0	35.0	39.0
11	37.06227768813007	39.0	37.0	39.0	33.0	39.0
12	36.4955776204605	39.0	35.0	39.0	33.0	39.0
13	36.282368174483366	39.0	35.0	39.0	32.0	39.0
14	37.309858934985165	39.0	35.0	41.0	32.0	41.0
15	37.53098138219423	40.0	35.0	41.0	33.0	41.0
16	37.65870551593705	40.0	35.0	41.0	33.0	41.0
17	37.61650703589957	40.0	35.0	41.0	33.0	41.0
18	37.54380086061805	39.0	36.0	41.0	33.0	41.0
19	37.51157453195218	39.0	36.0	41.0	33.0	41.0
20	37.39916410736904	39.0	35.0	41.0	32.0	41.0
21	37.2815834678049	39.0	35.0	41.0	32.0	41.0
22	37.20085064112575	39.0	35.0	41.0	32.0	41.0
23	37.11937518435619	39.0	35.0	41.0	32.0	41.0
24	37.14959658528968	39.0	35.0	41.0	32.0	41.0
25	37.140996043933164	39.0	35.0	41.0	32.0	41.0
26	37.0359620356393	39.0	35.0	41.0	32.0	41.0
27	36.98274209220412	39.0	35.0	41.0	32.0	41.0
28	36.952880728055106	39.0	35.0	41.0	32.0	41.0
29	36.9058816129648	39.0	35.0	41.0	31.0	41.0
30	36.8437210452345	39.0	35.0	41.0	31.0	41.0
31	36.77835484878455	39.0	35.0	41.0	31.0	41.0
32	36.68980575364808	39.0	35.0	41.0	31.0	41.0
33	36.61290970450957	39.0	35.0	41.0	31.0	41.0
34	36.53765420852636	39.0	35.0	41.0	30.0	41.0
35	36.43962356635955	39.0	35.0	41.0	30.0	41.0
36	36.29591770339909	38.0	35.0	41.0	30.0	41.0
37	36.23182204639703	38.0	35.0	41.0	30.0	41.0
38	36.13618846841219	38.0	35.0	41.0	30.0	41.0
39	36.09882489198896	38.0	35.0	41.0	30.0	41.0
40	35.969962087692814	38.0	35.0	40.0	29.0	41.0
41	35.918987819478424	38.0	35.0	40.0	29.0	41.0
42	35.89041911751948	38.0	35.0	40.0	29.0	41.0
43	35.8067535092742	38.0	35.0	40.0	29.0	41.0
44	35.64261577568407	38.0	35.0	40.0	28.0	41.0
45	35.629596758801384	38.0	34.0	40.0	28.0	41.0
46	35.568903232523034	38.0	34.0	40.0	28.0	41.0
47	35.501840959172696	38.0	34.0	40.0	28.0	41.0
48	35.38482987177485	38.0	34.0	40.0	28.0	41.0
49	35.286883816563424	37.0	34.0	40.0	27.0	41.0
50	35.140411309492826	37.0	34.0	40.0	27.0	41.0
51	34.7972411639165	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	2.0
11	4.0
12	7.0
13	16.0
14	18.0
15	50.0
16	124.0
17	289.0
18	647.0
19	1278.0
20	2330.0
21	3734.0
22	5797.0
23	8656.0
24	12605.0
25	18244.0
26	24183.0
27	29780.0
28	34165.0
29	40041.0
30	49114.0
31	61325.0
32	77312.0
33	100981.0
34	172950.0
35	268290.0
36	155205.0
37	207694.0
38	334818.0
39	695471.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.53653288914337	24.575069838460603	31.492070515156247	12.396326757239775
2	29.295759373969776	26.276352089948467	31.294050283691632	13.133838252390124
3	29.32000763451495	25.61600992486943	31.37963493137612	13.684347509239497
4	26.702583589263096	28.075104540801277	30.687973903839815	14.534337966095814
5	23.624746239133827	32.51140839449621	29.671151944198638	14.192693422171326
6	23.03176999288602	41.43073412801693	25.56790380511165	9.96959207398539
7	90.7777662103309	3.176261863862718	4.630073048427116	1.4158988773792793
8	91.50026894314021	2.5307115714954973	4.477990040428227	1.4910294449360608
9	86.13342182430205	6.059679350372182	5.618135443235646	2.188763382090122
10	41.301251019381255	40.75690142800132	10.510341297520517	7.431506255096906
11	34.92213662311523	20.952405739767148	27.948571131122797	16.17688650599483
12	33.55404022001284	20.895971058247877	28.620148178994675	16.92984054274461
13	23.845713393368385	29.94438949907171	28.35771172765603	17.852185379903872
14	17.028004788923013	33.71640379643607	30.14431835927333	19.111273055367583
15	15.653618586573664	29.020352922804644	39.04438429372061	16.281644196901084
16	20.394088456266374	26.547681016084535	36.72440268596117	16.333827841687924
17	20.689275241615046	26.37017854354276	28.16242430551941	24.778121909322785
18	22.228193916679682	28.319365641212503	31.151076640119374	18.301363801988444
19	25.96199226137803	28.39909426890844	26.991220307809762	18.64769316190377
20	27.602935818020924	28.5327850363507	27.073942012388734	16.79033713323964
21	24.822410771606545	27.49947946488991	30.085801537313696	17.592308226189857
22	23.816129647944752	23.796869848871307	30.074479898669164	22.312520604514773
23	20.54521714989676	27.871705446532367	30.526911665191818	21.056165738379054
24	20.24144153523155	26.470164662606493	34.94356531848073	18.344828483681226
25	19.2683445248382	29.03783422691861	31.716030746273837	19.97779050196936
26	18.633031422969477	34.458296462096364	26.600298440129787	20.308373674804365
27	18.86545034962608	32.84468099873337	30.624555376260126	17.665313275380424
28	16.721062585671405	29.839371540610415	35.11187167074419	18.32769420297399
29	17.870360730831294	27.303454618013983	34.73699963562542	20.0891850155293
30	20.160151302205335	29.713011642635294	31.325586035778112	18.801251019381258
31	25.23502160220707	27.920722502732808	27.819912203078097	19.024343691982022
32	25.691487515832947	28.26271407006403	27.9614543750976	18.084344039005433
33	24.050500581264206	28.290215675047282	28.093236513802854	19.566047229885655
34	19.735871809553554	28.457177311609666	30.267945447920464	21.539005430916315
35	20.985503097183905	26.772075026460534	30.947026876962852	21.29539499939271
36	24.803194350458938	27.454973712976937	28.941448475699687	18.80038346086444
37	20.446272101053218	31.17532490066455	29.816641507469676	18.561761490812557
38	20.124971804348206	31.203043395277014	27.486335953360054	21.185648847014733
39	20.511686013221592	29.146539309076395	29.65540575711832	20.686368920583693
40	22.73233217080492	27.734197421616084	28.09093748373328	21.442532923845715
41	18.635720854371627	26.342676938559507	30.28299758818732	24.738604618881542
42	21.117415369666684	26.65243870699079	28.45500841531761	23.775137508024915
43	21.62970867385005	26.451555532420663	28.774486839137296	23.144248954591987
44	20.031839397567367	29.001006367879512	29.662736626585463	21.304417607967657
45	19.288688772057675	31.98215432130897	26.969704856592575	21.759452050040775
46	21.047186507729947	30.21602207068867	28.041963805458682	20.69482761612271
47	20.595145142539863	28.13913035934274	29.569300574323737	21.69642392379366
48	21.22082834487186	26.412949178422085	31.138670553328822	21.227551923377234
49	20.648369857546893	26.412732288792878	31.613398573733797	21.32549927992643
50	19.267607100098903	30.052010133083478	29.299620009369633	21.38076275744799
51	18.598415838148284	30.111698159040827	28.067166380372356	23.222719622438532
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1004.0
1	2901.5
2	4799.0
3	9883.0
4	14967.0
5	10764.5
6	6562.0
7	6054.0
8	5546.0
9	5738.5
10	5931.0
11	6012.5
12	6094.0
13	6102.0
14	6110.0
15	5855.0
16	5600.0
17	5815.0
18	6030.0
19	6511.0
20	6992.0
21	8810.5
22	10629.0
23	12240.0
24	13851.0
25	17521.5
26	26039.0
27	30886.0
28	40530.5
29	50175.0
30	58419.5
31	66664.0
32	73626.5
33	80589.0
34	87601.5
35	94614.0
36	95222.5
37	95831.0
38	108714.0
39	121597.0
40	143575.5
41	165554.0
42	183612.5
43	201671.0
44	206996.5
45	212322.0
46	203808.5
47	195295.0
48	190419.0
49	185543.0
50	170048.0
51	154553.0
52	139308.0
53	124063.0
54	112628.0
55	101193.0
56	93739.5
57	86286.0
58	79127.5
59	71969.0
60	62664.5
61	53360.0
62	46241.0
63	39122.0
64	31347.0
65	23572.0
66	18831.0
67	14090.0
68	11854.5
69	9619.0
70	7400.0
71	5181.0
72	4026.5
73	2872.0
74	2353.0
75	1353.0
76	872.0
77	598.0
78	324.0
79	237.0
80	150.0
81	129.0
82	108.0
83	74.0
84	40.0
85	37.5
86	35.0
87	24.0
88	13.0
89	12.5
90	12.0
91	8.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2305320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.248721617828885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.02999742782146	15.21226419945868
2	8.485456882437138	3.266683946602453
3	2.791303089465964	1.6118704836034816
4	1.3000506452380405	1.0009725183706346
5	0.7684404839541646	0.739574847775171
6	0.49419812264171376	0.5707609252070404
7	0.35927071438610947	0.4840851376659712
8	0.29174180370696967	0.44925254110709906
9	0.24372808397614434	0.42223086350132616
>10	3.225113780882772	15.805274995713742
>50	1.7209616806675074	24.394237255030138
>100	1.2838532404829022	34.64620527558782
>500	0.003620950362563909	0.4713807872515052
>1k	0.0020367845789421987	0.5172947831634437
>5k	2.263093976602443E-4	0.4079114399614726
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9364	0.4061908975760415	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.337792584109798E-5	0.0	0.0	0.03535300956049486	0.0
2	4.337792584109798E-5	0.0	0.0	0.14050110179931635	0.0
3	4.337792584109798E-5	0.0	0.0	0.21133725469782938	0.0
4	4.337792584109798E-5	0.0	0.0	0.3279804972845418	0.0
5	4.337792584109798E-5	0.0	0.0	0.6020422327485989	0.0
6	4.337792584109798E-5	0.0	0.0	0.810603300192598	0.0
7	4.337792584109798E-5	0.0	0.0	0.950974268214391	0.0
8	4.337792584109798E-5	0.0	0.0	1.275961688615897	0.0
9	4.337792584109798E-5	0.0	0.0	1.381760449742335	0.0
10	4.337792584109798E-5	0.0	0.0	1.613702219214686	0.0
11	4.337792584109798E-5	0.0	0.0	2.004624086894661	0.0
12	4.337792584109798E-5	0.0	0.0	2.322974684642479	0.0
13	4.337792584109798E-5	0.0	0.0	2.4407023753752193	0.0
14	4.337792584109798E-5	0.0	0.0	2.4900664549823888	0.0
15	4.337792584109798E-5	0.0	0.0	2.570402373640102	0.0
16	4.337792584109798E-5	0.0	0.0	2.75640691964673	0.0
17	4.337792584109798E-5	0.0	0.0	3.000364374577065	0.0
18	4.337792584109798E-5	0.0	0.0	3.2669217288706123	0.0
19	4.337792584109798E-5	0.0	0.0	3.4450314923741607	0.0
20	4.337792584109798E-5	0.0	0.0	3.6481269411621815	0.0
21	4.337792584109798E-5	0.0	0.0	3.8899588777263028	0.0
22	4.337792584109798E-5	0.0	0.0	4.162198740305033	0.0
23	4.337792584109798E-5	0.0	0.0	4.443114188051984	0.0
24	8.675585168219596E-5	0.0	0.0	4.635625422934777	0.0
25	8.675585168219596E-5	0.0	0.0	4.8039317751982376	0.0
26	8.675585168219596E-5	0.0	0.0	4.974103378272864	0.0
27	8.675585168219596E-5	0.0	0.0	5.1533409678482816	0.0
28	8.675585168219596E-5	0.0	0.0	5.334747453715753	0.0
29	8.675585168219596E-5	0.0	0.0	5.547299290337134	0.0
30	8.675585168219596E-5	0.0	0.0	5.8161556747002585	0.0
31	8.675585168219596E-5	0.0	0.0	6.053563062828588	0.0
32	8.675585168219596E-5	0.0	0.0	6.2574826922075895	0.0
33	8.675585168219596E-5	0.0	0.0	6.471856401714295	0.0
34	8.675585168219596E-5	0.0	0.0	6.699026599344125	0.0
35	8.675585168219596E-5	0.0	0.0	6.959120642687349	0.0
36	8.675585168219596E-5	0.0	0.0	7.188893515867646	0.0
37	8.675585168219596E-5	0.0	0.0	7.433067860427186	0.0
38	8.675585168219596E-5	0.0	0.0	7.688997622889664	0.0
39	8.675585168219596E-5	0.0	0.0	8.051723838772926	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCA	20	7.0347654E-4	45.0	35
TGCGGTA	20	7.0347654E-4	45.0	15
CGCGTAG	20	7.0347654E-4	45.0	36
TAGGACG	75	0.0	45.0	1
CGCATCG	60	0.0	44.999996	21
CGACGGT	535	0.0	42.897194	28
TCGTTAG	135	0.0	41.666668	1
TATAGCG	190	0.0	41.447372	1
CGTTTTT	3670	0.0	41.260216	1
CGGGGTA	290	0.0	40.344826	6
GACCGAT	605	0.0	40.165287	9
TACGGGA	1055	0.0	39.66825	4
CGAGGGA	1890	0.0	39.285713	4
TTTACGG	235	0.0	39.255318	2
TAGGGAC	2855	0.0	39.089314	5
TATGGGA	2950	0.0	38.898308	4
GGGCGAT	2335	0.0	38.832977	7
AGGGATT	5320	0.0	38.825188	6
ACGTCGA	35	6.2510244E-6	38.571426	38
TCGTCCC	170	0.0	38.38235	38
>>END_MODULE