FastQCFastQC Report
Thu 26 May 2016
SRR1548100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548100_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1233502
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53380.4327516291015337No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG207.0334884E-445.01
GCGATAT502.1827873E-1145.09
TCGTAAG406.8157533E-945.01
GGCGATA1750.043.7142878
TCACGAC1550.043.54838625
CGTTAGG3000.042.02
CGTTTTT25000.041.581
CGTAAGG1650.040.9090922
TAGTCGG556.184564E-1140.9090921
TAAGGGA17850.040.5882344
CACGACG1500.040.526
GGGCGAT9800.039.7193877
GGTCGAA403.458499E-739.3759
ATAGCGG2600.038.942312
GGCACCG2200.038.8636368
AAGGGAT23700.038.8291175
ATGTCGC356.2486597E-638.5714333
TACGCGG1400.038.571432
TACGACG356.2486597E-638.571431
GCGATAC356.2486597E-638.571439
Produced by FastQC (version 0.11.5)