##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548100_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1233502
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62554985723574	31.0	31.0	33.0	30.0	34.0
2	32.05797639566048	33.0	31.0	34.0	30.0	34.0
3	32.14548334741249	33.0	31.0	34.0	30.0	34.0
4	35.8423067007593	37.0	35.0	37.0	35.0	37.0
5	35.757185638936946	37.0	35.0	37.0	35.0	37.0
6	35.81433430995653	37.0	35.0	37.0	35.0	37.0
7	36.13659321184724	37.0	35.0	37.0	35.0	37.0
8	36.172658009472215	37.0	35.0	37.0	35.0	37.0
9	38.00160275378556	39.0	39.0	39.0	35.0	39.0
10	37.47586870552298	39.0	37.0	39.0	35.0	39.0
11	37.13720204750378	39.0	37.0	39.0	33.0	39.0
12	36.645801141789796	39.0	35.0	39.0	33.0	39.0
13	36.45865349225214	39.0	35.0	39.0	32.0	39.0
14	37.48823998663966	40.0	35.0	41.0	32.0	41.0
15	37.685921871225176	40.0	35.0	41.0	33.0	41.0
16	37.802471337703544	40.0	35.0	41.0	33.0	41.0
17	37.735569135680365	40.0	35.0	41.0	33.0	41.0
18	37.64402084471691	39.0	36.0	41.0	33.0	41.0
19	37.60604928082808	39.0	36.0	41.0	33.0	41.0
20	37.50594891617525	39.0	35.0	41.0	33.0	41.0
21	37.38676953908465	39.0	35.0	41.0	32.0	41.0
22	37.32824186746353	39.0	35.0	41.0	32.0	41.0
23	37.24690596367091	39.0	35.0	41.0	32.0	41.0
24	37.27985929491805	39.0	35.0	41.0	32.0	41.0
25	37.268392754936755	39.0	35.0	41.0	32.0	41.0
26	37.163377116534875	39.0	35.0	41.0	32.0	41.0
27	37.11818951246127	39.0	35.0	41.0	32.0	41.0
28	37.082087422638956	39.0	35.0	41.0	32.0	41.0
29	37.0318921250229	39.0	35.0	41.0	32.0	41.0
30	36.944645407952315	39.0	35.0	41.0	32.0	41.0
31	36.879976684269664	39.0	35.0	41.0	31.0	41.0
32	36.76973203124113	39.0	35.0	41.0	31.0	41.0
33	36.67724332834483	39.0	35.0	41.0	31.0	41.0
34	36.574481435782026	39.0	35.0	41.0	30.0	41.0
35	36.46038433662856	39.0	35.0	41.0	30.0	41.0
36	36.32247292667543	39.0	35.0	41.0	30.0	41.0
37	36.26008226983013	39.0	35.0	41.0	30.0	41.0
38	36.1324383746439	39.0	35.0	41.0	30.0	41.0
39	36.07707162209709	38.0	35.0	41.0	30.0	41.0
40	35.96691938886195	38.0	35.0	41.0	29.0	41.0
41	35.88774318971514	38.0	35.0	40.0	29.0	41.0
42	35.852333437643395	38.0	35.0	40.0	29.0	41.0
43	35.77188362888751	38.0	35.0	40.0	29.0	41.0
44	35.610582714904396	38.0	35.0	40.0	28.0	41.0
45	35.58922806772912	38.0	34.0	40.0	28.0	41.0
46	35.524913619921165	38.0	34.0	40.0	28.0	41.0
47	35.45770497331986	38.0	34.0	40.0	28.0	41.0
48	35.33360545828057	38.0	34.0	40.0	27.0	41.0
49	35.236910033384625	38.0	34.0	40.0	27.0	41.0
50	35.08975664409137	37.0	34.0	40.0	27.0	41.0
51	34.73565669127411	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	13.0
15	29.0
16	83.0
17	176.0
18	370.0
19	732.0
20	1251.0
21	2012.0
22	3161.0
23	4601.0
24	7082.0
25	10254.0
26	13964.0
27	16423.0
28	18437.0
29	20721.0
30	25016.0
31	30958.0
32	39249.0
33	52182.0
34	88612.0
35	132709.0
36	83451.0
37	114032.0
38	187194.0
39	380712.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.75673651116902	24.585691794581606	30.29034407726943	12.367227616979948
2	30.467076664650726	25.32464479182036	30.973196638513762	13.235081905015154
3	29.698857399501584	25.104134407564803	31.62054054229341	13.57646765064021
4	26.43724939238039	27.825491973259876	31.088397100288446	14.648861534071287
5	23.78496346175361	31.613973872762262	29.5809005579237	15.020162107560425
6	23.286788347323313	40.22409367799971	26.103078876240165	10.38603909843681
7	89.94991495757607	3.3041697540822796	5.324271869846989	1.42164341849466
8	91.09381257590178	2.2478277295051003	5.1209483243642895	1.5374113702288281
9	85.43010064029082	5.622447308557263	6.5491584123900894	2.3982936387618343
10	42.16312579955282	36.85985105820663	12.559687783238294	8.417335359002255
11	32.82726740613311	22.509570312816678	28.464080317664664	16.19908196338555
12	32.523335997833804	21.761618546220436	28.963795761984983	16.75124969396077
13	25.70786265445861	27.555934242506297	28.608790257332377	18.12741284570272
14	18.45898912202818	31.53379564848699	30.872021285737677	19.135193943747154
15	16.955383939385587	29.399141630901287	37.39775046980062	16.24772395991251
16	23.392017199809974	26.30607814174602	34.4706372587965	15.831267399647508
17	23.170209695647028	25.836926085243476	28.39241444278161	22.600449776327885
18	23.37061472133811	27.352124277058326	31.410001767325873	17.86725923427769
19	26.35966540791989	27.987794101671504	27.276891322429957	18.37564916797865
20	27.42265517202242	28.745798547549985	27.534693904022856	16.29685237640474
21	26.501618967784406	26.253382645508477	30.521150350789867	16.72384803591725
22	24.474950182488556	22.934944572444955	31.606434363300583	20.9836708817659
23	21.633527955366105	26.198092909456168	31.439430175224686	20.728948959953044
24	20.982535901846937	25.765503420343055	34.472420798669155	18.779539879140852
25	20.72384155031771	28.35033911578579	31.89050362301804	19.035315710878457
26	19.84398890313919	33.46626110050896	27.43295106128729	19.256798935064555
27	18.944760527344098	32.23521323840578	30.84413320772889	17.975893026521238
28	17.520279659052033	29.102587592075245	34.539222473899514	18.837910274973208
29	18.953353946730527	27.15544847110098	34.340519918086876	19.550677664081615
30	20.280145471997614	28.15658182962006	32.32333632211379	19.239936376268542
31	24.90372938187372	26.81000922576534	29.041785096416543	19.244476295944395
32	25.758450330846642	27.263514773385044	28.84186649069073	18.136168405077576
33	24.33956329215518	27.369067905848553	28.465134227589417	19.82623457440685
34	20.13989438201154	28.034247208354753	30.925851761894187	20.900006647739524
35	21.050472556996258	27.32107446927528	31.370196400167977	20.25825657356048
36	24.942237629124232	27.022250470611315	29.060106915108364	18.975404985156082
37	20.974428902425775	30.12220490927457	29.551958569990155	19.351407618309498
38	20.81869344354529	30.070968672932835	28.57003880009923	20.540299083422646
39	20.551405672629635	29.069592104431123	29.676806361076025	20.702195861863217
40	22.939889842091866	27.032303149893554	28.600602187917	21.427204820097575
41	19.281768493281728	26.387715625917103	30.42832520741758	23.902190673383586
42	20.550027482728037	27.06602826748558	29.000925819333894	23.383018430452484
43	21.13592033089529	27.162258350614753	29.045433246156065	22.65638807233389
44	20.638880196383955	28.4077366716876	29.75252573566966	21.200857396258783
45	19.667499525740535	30.792815901392945	27.805873034660667	21.733811538205856
46	21.650309444167906	29.845269809047736	28.828327801657395	19.676092945126964
47	20.9382716850074	28.436354379644296	29.018518008077816	21.606855927270487
48	21.16478124883462	27.454677819736006	31.250050668746383	20.130490262682997
49	21.529758362775254	26.019982132173276	31.38178940934024	21.068470095711238
50	20.238070145001792	29.051189215745087	29.664240511973226	21.04650012727989
51	19.150272962670513	30.194600414105533	28.823544671998913	21.831581951225047
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	424.0
1	1272.0
2	2120.0
3	5670.0
4	9220.0
5	6764.5
6	4309.0
7	3945.5
8	3582.0
9	3628.5
10	3675.0
11	3703.5
12	3732.0
13	3517.0
14	3302.0
15	3249.0
16	3196.0
17	3218.0
18	3240.0
19	3635.0
20	4030.0
21	4718.0
22	5406.0
23	6605.0
24	7804.0
25	10219.5
26	15243.0
27	17851.0
28	21842.5
29	25834.0
30	28393.5
31	30953.0
32	36362.0
33	41771.0
34	45643.0
35	49515.0
36	53343.5
37	57172.0
38	61770.0
39	66368.0
40	74436.0
41	82504.0
42	91592.5
43	100681.0
44	102230.5
45	103780.0
46	100442.5
47	97105.0
48	95257.0
49	93409.0
50	89288.0
51	85167.0
52	77070.5
53	68974.0
54	63995.5
55	59017.0
56	54010.5
57	49004.0
58	45683.5
59	42363.0
60	37870.5
61	33378.0
62	28228.5
63	23079.0
64	18929.0
65	14779.0
66	12452.5
67	10126.0
68	8048.0
69	5970.0
70	4864.0
71	3758.0
72	2825.0
73	1892.0
74	1453.5
75	834.5
76	654.0
77	514.0
78	374.0
79	280.0
80	186.0
81	128.5
82	71.0
83	56.0
84	41.0
85	30.0
86	19.0
87	15.0
88	11.0
89	7.5
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1233502.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.816230434518136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09930047819228	16.46549265963241
2	6.134336457874123	2.55387522539947
3	1.9257741766190577	1.202620770760402
4	1.0481567035206105	0.8727468588787975
5	0.7186074809910623	0.7479349458139283
6	0.5313031279223396	0.6635837004847013
7	0.48093004765993225	0.7007805486481058
8	0.4141380651263924	0.689663471630117
9	0.3462012258392733	0.648594404440467
>10	6.770297566459496	36.65849885847335
>50	2.345991285831926	33.30433608817165
>100	0.1830122934153465	4.762682419711551
>500	7.804362192552087E-4	0.1198123338235215
>1k	7.804362192552087E-4	0.17577890874176308
>5k	3.9021810962760437E-4	0.4335988053898019
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5338	0.4327516291015337	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03121194777146693	0.0
2	0.0	0.0	0.0	0.100526792822387	0.0
3	0.0	0.0	0.0	0.14000788000343736	0.0
4	0.0	0.0	0.0	0.20032395569686956	0.0
5	0.0	0.0	0.0	0.3533030347741633	0.0
6	0.0	0.0	0.0	0.47507016607999014	0.0
7	0.0	0.0	0.0	0.5558158803147462	0.0
8	0.0	0.0	0.0	0.7516809863299776	0.0
9	0.0	0.0	0.0	0.8166180516934711	0.0
10	0.0	0.0	0.0	0.9647329311180687	0.0
11	8.106999421160241E-5	0.0	0.0	1.2123206934403026	0.0
12	8.106999421160241E-5	0.0	0.0	1.3973224202311791	0.0
13	8.106999421160241E-5	0.0	0.0	1.4740957047495666	0.0
14	8.106999421160241E-5	0.0	0.0	1.5084693822952862	0.0
15	8.106999421160241E-5	0.0	0.0	1.5652994482376195	0.0
16	8.106999421160241E-5	0.0	0.0	1.6947682289935484	0.0
17	8.106999421160241E-5	0.0	0.0	1.8558543074920024	0.0
18	8.106999421160241E-5	0.0	0.0	2.026344505319002	0.0
19	8.106999421160241E-5	0.0	0.0	2.145517396810058	0.0
20	8.106999421160241E-5	0.0	0.0	2.2732837076875434	0.0
21	8.106999421160241E-5	0.0	0.0	2.4355858360991713	0.0
22	8.106999421160241E-5	0.0	0.0	2.6080217137872497	0.0
23	8.106999421160241E-5	0.0	0.0	2.7791604715679425	0.0
24	8.106999421160241E-5	0.0	0.0	2.9110613521502193	0.0
25	8.106999421160241E-5	0.0	0.0	3.030639593612333	0.0
26	8.106999421160241E-5	0.0	0.0	3.1477857352480987	0.0
27	8.106999421160241E-5	0.0	0.0	3.2835779755525327	0.0
28	8.106999421160241E-5	0.0	0.0	3.415803136111656	0.0
29	8.106999421160241E-5	0.0	0.0	3.567322955293141	0.0
30	8.106999421160241E-5	0.0	0.0	3.7760781903880174	0.0
31	8.106999421160241E-5	0.0	0.0	3.943893078406034	0.0
32	8.106999421160241E-5	0.0	0.0	4.08681947820109	0.0
33	8.106999421160241E-5	0.0	0.0	4.239068927330479	0.0
34	8.106999421160241E-5	0.0	0.0	4.4055056254468985	0.0
35	8.106999421160241E-5	0.0	0.0	4.593831222000451	0.0
36	8.106999421160241E-5	0.0	0.0	4.7601868501226585	0.0
37	8.106999421160241E-5	0.0	0.0	4.933676637735488	0.0
38	8.106999421160241E-5	0.0	0.0	5.1388647930850535	0.0
39	8.106999421160241E-5	0.0	0.0	5.46752254961889	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAG	20	7.0334884E-4	45.0	1
GCGATAT	50	2.1827873E-11	45.0	9
TCGTAAG	40	6.8157533E-9	45.0	1
GGCGATA	175	0.0	43.714287	8
TCACGAC	155	0.0	43.548386	25
CGTTAGG	300	0.0	42.0	2
CGTTTTT	2500	0.0	41.58	1
CGTAAGG	165	0.0	40.909092	2
TAGTCGG	55	6.184564E-11	40.909092	1
TAAGGGA	1785	0.0	40.588234	4
CACGACG	150	0.0	40.5	26
GGGCGAT	980	0.0	39.719387	7
GGTCGAA	40	3.458499E-7	39.375	9
ATAGCGG	260	0.0	38.94231	2
GGCACCG	220	0.0	38.863636	8
AAGGGAT	2370	0.0	38.829117	5
ATGTCGC	35	6.2486597E-6	38.57143	33
TACGCGG	140	0.0	38.57143	2
TACGACG	35	6.2486597E-6	38.57143	1
GCGATAC	35	6.2486597E-6	38.57143	9
>>END_MODULE