FastQCFastQC Report
Thu 26 May 2016
SRR1548099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548099_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1970025
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63890.32431060519536553No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCC253.891823E-545.00000430
TACCGCG302.1662945E-645.0000041
TCCGATC207.034516E-445.017
CGCGTAG453.8562575E-1045.024
CGAAGTA207.034516E-445.036
CGACGGT4250.043.41176228
CGCGAGG2700.043.3333322
CGGTCTA4350.042.93103431
ACGGTCT4450.041.46067430
GGCGATA4300.040.8139538
TACGGGA7850.040.7006384
GGGCGAT17150.040.4081657
CACGACG4600.040.108726
TCACGAC4550.040.05494325
AGTCCGC451.929584E-840.013
ACGGGTA1800.040.05
GCGCGAG1750.039.8571431
CGTTTTT31550.039.7939761
CGGGTAT1700.039.7058836
TCTCACG4550.039.5604423
Produced by FastQC (version 0.11.5)