##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548099_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1970025
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.532785624547913	31.0	31.0	33.0	30.0	34.0
2	31.94816512480806	31.0	31.0	34.0	30.0	34.0
3	32.05390083882184	33.0	31.0	34.0	30.0	34.0
4	35.75306303219502	37.0	35.0	37.0	35.0	37.0
5	35.64950495552087	37.0	35.0	37.0	33.0	37.0
6	35.712988413852614	37.0	35.0	37.0	33.0	37.0
7	36.07764520754813	37.0	35.0	37.0	35.0	37.0
8	36.113310744787505	37.0	35.0	37.0	35.0	37.0
9	37.947919442646665	39.0	38.0	39.0	35.0	39.0
10	37.3715313257446	39.0	37.0	39.0	35.0	39.0
11	37.01074554891435	39.0	37.0	39.0	33.0	39.0
12	36.445421758607125	38.0	35.0	39.0	32.0	39.0
13	36.21276024415934	39.0	35.0	39.0	32.0	39.0
14	37.17389677795967	39.0	35.0	41.0	32.0	41.0
15	37.42865496630753	39.0	35.0	41.0	32.0	41.0
16	37.543248435933556	39.0	35.0	41.0	33.0	41.0
17	37.496452583089045	39.0	35.0	41.0	33.0	41.0
18	37.43935076966029	39.0	35.0	41.0	32.0	41.0
19	37.413395261481455	39.0	35.0	41.0	32.0	41.0
20	37.29170086674027	39.0	35.0	41.0	32.0	41.0
21	37.17488407507519	39.0	35.0	41.0	32.0	41.0
22	37.11642999454322	39.0	35.0	41.0	32.0	41.0
23	37.03629953934595	39.0	35.0	41.0	32.0	41.0
24	37.06498546972754	39.0	35.0	41.0	32.0	41.0
25	37.052522176114515	39.0	35.0	41.0	32.0	41.0
26	36.92804761360897	39.0	35.0	41.0	32.0	41.0
27	36.88242788797096	39.0	35.0	41.0	31.0	41.0
28	36.872986383421534	39.0	35.0	41.0	31.0	41.0
29	36.81966929353688	39.0	35.0	41.0	31.0	41.0
30	36.74495399804571	38.0	35.0	41.0	31.0	41.0
31	36.672942729153185	38.0	35.0	41.0	31.0	41.0
32	36.599436555373664	39.0	35.0	41.0	31.0	41.0
33	36.518297483534475	39.0	35.0	41.0	30.0	41.0
34	36.44273346784939	38.0	35.0	41.0	30.0	41.0
35	36.343366200936536	38.0	35.0	41.0	30.0	41.0
36	36.20892323701476	38.0	35.0	41.0	30.0	41.0
37	36.16371315084834	38.0	35.0	41.0	30.0	41.0
38	36.039988325021255	38.0	35.0	41.0	30.0	41.0
39	35.98581591604167	38.0	35.0	40.0	29.0	41.0
40	35.8854435857413	38.0	35.0	40.0	29.0	41.0
41	35.82022918490882	38.0	35.0	40.0	29.0	41.0
42	35.79192396035583	38.0	35.0	40.0	29.0	41.0
43	35.7076732528775	38.0	35.0	40.0	28.0	41.0
44	35.55529549117397	38.0	34.0	40.0	28.0	41.0
45	35.51587416403345	38.0	34.0	40.0	28.0	41.0
46	35.45996776690651	38.0	34.0	40.0	28.0	41.0
47	35.38519460412939	38.0	34.0	40.0	27.0	41.0
48	35.27935787616909	38.0	34.0	40.0	27.0	41.0
49	35.190829050392765	37.0	34.0	40.0	27.0	41.0
50	35.029863580411416	37.0	34.0	40.0	26.0	41.0
51	34.66351543762135	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	3.0
14	14.0
15	36.0
16	124.0
17	273.0
18	593.0
19	1187.0
20	2108.0
21	3478.0
22	5415.0
23	8032.0
24	11513.0
25	16388.0
26	22031.0
27	26861.0
28	30994.0
29	36027.0
30	43709.0
31	54084.0
32	67464.0
33	88211.0
34	147556.0
35	227541.0
36	131762.0
37	176492.0
38	289237.0
39	578802.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.37862463674318	24.47842032461517	31.16995977208411	11.97299526655753
2	29.242319259907866	25.801550741741856	32.06223271278284	12.893897285567443
3	29.73693227243309	25.17856372381061	31.695536858669303	13.38896714508699
4	26.514739660664205	27.534980520551773	31.065798657377446	14.884481161406582
5	23.080976129744545	32.279641121305566	30.148043806550678	14.49133894239921
6	21.728404461872312	41.613177497747486	26.114186368193298	10.544231672186902
7	91.60310148348371	2.8952932069389985	4.207053209984645	1.2945520995926447
8	92.80318777680486	1.9833758454841945	3.9433509727033917	1.2700854050075507
9	87.40620042892857	5.277039631476757	5.2034872653900335	2.113272674204642
10	44.078273118361444	36.64400197967031	10.640677148767146	8.637047753201102
11	33.73764292331315	21.890229819418536	27.269450895293208	17.102676361975103
12	33.499422596159945	22.133780028172232	27.07874265555006	17.288054720117767
13	24.677757896473395	29.873631045291305	27.350465095620613	18.098145962614687
14	17.124300453039936	34.14789152421924	29.551604675067576	19.176203347673255
15	17.02120531465337	28.72775726196368	38.205098920064465	16.045938503318485
16	22.391187929087195	26.006471999086305	34.999454321645665	16.602885750180835
17	22.032563038540122	25.511199096458164	26.903465692059747	25.552772172941967
18	22.690574992703137	27.43782439309146	31.859341886524284	18.01225872768112
19	27.09351404169998	27.459296201824852	26.30865090544536	19.13853885102981
20	27.815433814291694	28.61588050913059	26.67240263448433	16.896283042093373
21	25.77652567860814	26.974530780066242	29.730333371403916	17.518610169921704
22	24.479130975495234	23.001230948845826	29.879163970000384	22.64047410565856
23	20.686488750142765	27.90375756652834	30.128247103463153	21.281506579865738
24	20.558622352508216	26.326417177446988	34.316468065126074	18.79849240491872
25	20.27004733442469	28.635321886778087	31.80487557264502	19.289755206152208
26	18.947779850509512	33.82860623596147	26.394893465818964	20.828720447710054
27	17.759470057486578	33.81789571198335	30.449765865915406	17.972868364614662
28	16.457659166761843	30.09971954670626	34.8119440108628	18.63067727566909
29	18.252154160480195	28.1034504638266	34.3748429588457	19.269552416847503
30	20.578165251710004	29.243131432342228	30.801538051547567	19.377165264400197
31	24.942881435514778	27.407317166025813	27.980761665461095	19.66903973299831
32	26.445349678303575	27.597619319551782	27.655994213271406	18.301036788873237
33	24.73506681387292	27.366962348193553	27.803911117879217	20.094059720054315
34	20.027664623545384	27.194629509777794	30.52088171469905	22.256824151977767
35	20.399690359259402	26.973921650740472	31.294069872209747	21.332318117790383
36	25.684699432748314	26.294894734838394	28.831766094338906	19.18863973807439
37	20.297153589421455	30.348853440946183	29.379576401314704	19.97441656831766
38	20.522429918402054	29.929011053159222	28.162586769203436	21.385972259235288
39	19.78441897945458	29.407900914963008	29.625461605817183	21.18221849976523
40	23.18173627238233	26.343472798568545	28.171520665981397	22.303270263067727
41	19.020570804939023	25.745815408433902	30.077841651755687	25.155772134871384
42	20.46014644484207	26.347685943071784	28.495831271176762	24.69633634090938
43	20.87080113196533	26.920267509295563	28.665270745295114	23.543660613443993
44	20.70359513204147	28.867349399119302	29.210339970304943	21.218715498534284
45	19.549295059707365	31.859849494295755	26.792857958655347	21.79799748734153
46	21.642161901498714	30.159160416745976	27.69502925089783	20.503648430857478
47	20.197662466212357	27.989746323016206	29.42693620639332	22.385655004378116
48	20.899582492607962	27.40351010773975	30.725853732820653	20.97105366683164
49	21.569472468623495	25.54541186025558	31.352140201266483	21.532975469854442
50	20.076445730384133	29.477950787426554	29.21145670740219	21.23414677478712
51	19.38285047144072	30.326366416669835	28.08142028654459	22.209362825344854
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	664.0
1	1669.0
2	2674.0
3	6698.0
4	10722.0
5	7837.5
6	4953.0
7	4644.5
8	4336.0
9	4367.5
10	4399.0
11	4362.0
12	4325.0
13	4217.0
14	4109.0
15	4259.5
16	4410.0
17	4161.5
18	3913.0
19	3919.5
20	3926.0
21	5224.0
22	6522.0
23	8012.5
24	9503.0
25	13066.0
26	21004.0
27	25379.0
28	29658.5
29	33938.0
30	41660.0
31	49382.0
32	56471.5
33	63561.0
34	69155.5
35	74750.0
36	78166.5
37	81583.0
38	90666.5
39	99750.0
40	121115.5
41	142481.0
42	155524.0
43	168567.0
44	174803.5
45	181040.0
46	179282.5
47	177525.0
48	171434.0
49	165343.0
50	156049.0
51	146755.0
52	133901.5
53	121048.0
54	108613.5
55	96179.0
56	88470.0
57	80761.0
58	71812.5
59	62864.0
60	53583.0
61	44302.0
62	37144.0
63	29986.0
64	24078.0
65	18170.0
66	14840.0
67	11510.0
68	8961.5
69	6413.0
70	5104.0
71	3795.0
72	2949.5
73	2104.0
74	1554.0
75	680.0
76	356.0
77	253.0
78	150.0
79	130.0
80	110.0
81	84.5
82	59.0
83	42.5
84	26.0
85	27.5
86	29.0
87	20.5
88	12.0
89	8.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1970025.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.076860624959654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22484374494084	16.106630065263435
2	7.948517229441602	3.19162545181179
3	2.5235863375294083	1.5199707352089098
4	1.1691631941166813	0.9389250598445306
5	0.7175273688979543	0.7202848489979119
6	0.48928736690617813	0.5894012562557301
7	0.3615588880569592	0.5081277182264472
8	0.28420383058761084	0.45647365566296866
9	0.24394021648200367	0.4407798354418507
>10	3.132614655654687	15.872840977794617
>50	1.6843611454075746	24.626834894254163
>100	1.2168505676731622	34.155673404040876
>500	0.0025324673608927164	0.33714652581805526
>1k	7.59740208267815E-4	0.21044329216723434
>5k	2.5324673608927167E-4	0.3248422792115149
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6389	0.32431060519536553	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5228233144249438E-4	0.0	0.0	0.04715676197002576	0.0
2	1.5228233144249438E-4	0.0	0.0	0.13649572974962246	0.0
3	1.5228233144249438E-4	0.0	0.0	0.19451529802921283	0.0
4	1.5228233144249438E-4	0.0	0.0	0.284006548140252	0.0
5	1.5228233144249438E-4	0.0	0.0	0.503496148526034	0.0
6	1.5228233144249438E-4	0.0	0.0	0.6726310579814977	0.0
7	1.5228233144249438E-4	0.0	0.0	0.7902945394093983	0.0
8	1.5228233144249438E-4	0.0	0.0	1.061001763937006	0.0
9	1.5228233144249438E-4	0.0	0.0	1.1460768264362127	0.0
10	1.5228233144249438E-4	0.0	0.0	1.3452621159629954	0.0
11	1.5228233144249438E-4	0.0	0.0	1.695663760612175	0.0
12	1.5228233144249438E-4	0.0	0.0	1.9812438928439995	0.0
13	1.5228233144249438E-4	0.0	0.0	2.094491186660068	0.0
14	1.5228233144249438E-4	0.0	0.0	2.1418509917386834	0.0
15	1.5228233144249438E-4	0.0	0.0	2.217890635905636	0.0
16	1.5228233144249438E-4	0.0	0.0	2.3906803213157195	0.0
17	1.5228233144249438E-4	0.0	0.0	2.616464258067791	0.0
18	1.5228233144249438E-4	0.0	0.0	2.8662072816334816	0.0
19	1.5228233144249438E-4	0.0	0.0	3.0247331886651185	0.0
20	1.5228233144249438E-4	0.0	0.0	3.2063552492988667	0.0
21	1.5228233144249438E-4	0.0	0.0	3.424474308701666	0.0
22	1.5228233144249438E-4	0.0	0.0	3.677009175010469	0.0
23	1.5228233144249438E-4	0.0	0.0	3.9233512265072776	0.0
24	1.5228233144249438E-4	0.0	0.0	4.108932627758531	0.0
25	1.5228233144249438E-4	0.0	0.0	4.281316226951435	0.0
26	1.5228233144249438E-4	0.0	0.0	4.447659293663786	0.0
27	1.5228233144249438E-4	0.0	0.0	4.639027423509854	0.0
28	1.5228233144249438E-4	0.0	0.0	4.827248385172777	0.0
29	1.5228233144249438E-4	0.0	0.0	5.038667021992107	0.0
30	1.5228233144249438E-4	0.0	0.0	5.325922259869799	0.0
31	1.5228233144249438E-4	0.0	0.0	5.5637365008058275	0.0
32	1.5228233144249438E-4	0.0	0.0	5.762617225669725	0.0
33	1.5228233144249438E-4	0.0	0.0	5.981396175175442	0.0
34	1.5228233144249438E-4	0.0	0.0	6.217484549688455	0.0
35	1.5228233144249438E-4	0.0	0.0	6.481136026192561	0.0
36	1.5228233144249438E-4	0.0	0.0	6.704838771081585	0.0
37	1.5228233144249438E-4	0.0	0.0	6.947627568178069	0.0
38	1.5228233144249438E-4	0.0	0.0	7.227319450260784	0.0
39	1.5228233144249438E-4	0.0	0.0	7.691882082714686	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCC	25	3.891823E-5	45.000004	30
TACCGCG	30	2.1662945E-6	45.000004	1
TCCGATC	20	7.034516E-4	45.0	17
CGCGTAG	45	3.8562575E-10	45.0	24
CGAAGTA	20	7.034516E-4	45.0	36
CGACGGT	425	0.0	43.411762	28
CGCGAGG	270	0.0	43.333332	2
CGGTCTA	435	0.0	42.931034	31
ACGGTCT	445	0.0	41.460674	30
GGCGATA	430	0.0	40.813953	8
TACGGGA	785	0.0	40.700638	4
GGGCGAT	1715	0.0	40.408165	7
CACGACG	460	0.0	40.1087	26
TCACGAC	455	0.0	40.054943	25
AGTCCGC	45	1.929584E-8	40.0	13
ACGGGTA	180	0.0	40.0	5
GCGCGAG	175	0.0	39.857143	1
CGTTTTT	3155	0.0	39.793976	1
CGGGTAT	170	0.0	39.705883	6
TCTCACG	455	0.0	39.56044	23
>>END_MODULE