FastQCFastQC Report
Thu 26 May 2016
SRR1548098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548098_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1196110
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42650.3565725560358161No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18690.15625653158990394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG750.045.0000041
TCGTGCG453.8562575E-1045.0000041
ACAACGC253.8909006E-545.039
ACACGGC207.0334016E-445.013
GCGACTT207.0334016E-445.018
GTCGATA207.0334016E-445.021
TGGCGTA207.0334016E-445.031
CGATTGA551.8189894E-1245.010
ACCCTCG351.2119926E-745.017
CGTTCCA253.8909006E-545.021
GTAATCG253.8909006E-545.043
CTACGCA351.2119926E-745.039
TGCGACC207.0334016E-445.026
TTGCGAC207.0334016E-445.043
TCGTATC253.8909006E-545.021
TCCGTCG207.0334016E-445.014
CGTGTAA207.0334016E-445.044
CGTCGGT207.0334016E-445.016
TTACGCG207.0334016E-445.01
TAGGCGA207.0334016E-445.05