FastQCFastQC Report
Thu 26 May 2016
SRR1548097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548097_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2829885
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125100.44206743383565056No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG551.8189894E-1245.01
AATCGGC302.1666328E-644.99999641
CGACGGT3800.042.03947428
CGTAAGG5150.039.7572862
CGTTTTT55350.039.51221
TACGGGA17100.039.4736864
TCGCACG403.4606092E-739.3751
AGGGATT63500.039.118116
TAGGGAC39550.039.026555
CGTTAGG5150.038.88352
ACGGGAC12450.038.8554235
CACGACG3900.038.65384726
GACCGAT8450.038.609479
GTCGATC356.2515282E-638.57142623
TTAGGGA50900.038.369354
ACGGGAT15550.037.9099665
ATAGGGA44050.037.8490334
TAGGGAT56900.037.724085
AAGGGAT50450.037.5520325
CGAAACG301.14034396E-437.49999642
Produced by FastQC (version 0.11.5)