##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548097_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2829885
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.557290137231725	31.0	31.0	33.0	30.0	34.0
2	31.98003841145488	31.0	31.0	34.0	30.0	34.0
3	32.07580626067844	33.0	31.0	34.0	30.0	34.0
4	35.78345056424554	37.0	35.0	37.0	35.0	37.0
5	35.693442666398106	37.0	35.0	37.0	33.0	37.0
6	35.749791952676524	37.0	35.0	37.0	33.0	37.0
7	36.100373336725696	37.0	35.0	37.0	35.0	37.0
8	36.130988361717876	37.0	35.0	37.0	35.0	37.0
9	37.970699869429325	39.0	38.0	39.0	35.0	39.0
10	37.39806069857963	39.0	37.0	39.0	35.0	39.0
11	37.03388476916906	39.0	37.0	39.0	33.0	39.0
12	36.603531592273185	39.0	35.0	39.0	33.0	39.0
13	36.43840050037369	39.0	35.0	39.0	32.0	39.0
14	37.520893605217175	40.0	36.0	41.0	32.0	41.0
15	37.703466041906296	40.0	36.0	41.0	33.0	41.0
16	37.79293999579488	40.0	36.0	41.0	33.0	41.0
17	37.72733344287842	40.0	36.0	41.0	33.0	41.0
18	37.65018366470722	39.0	36.0	41.0	33.0	41.0
19	37.620759147456525	39.0	36.0	41.0	33.0	41.0
20	37.52122542082099	39.0	35.0	41.0	33.0	41.0
21	37.405797055357375	39.0	35.0	41.0	32.0	41.0
22	37.322207086153675	39.0	35.0	41.0	32.0	41.0
23	37.229258786134416	39.0	35.0	41.0	32.0	41.0
24	37.24333780347965	39.0	35.0	41.0	32.0	41.0
25	37.22931461879193	39.0	35.0	41.0	32.0	41.0
26	37.121255104005996	39.0	35.0	41.0	32.0	41.0
27	37.07829116730892	39.0	35.0	41.0	32.0	41.0
28	37.028767953468076	39.0	35.0	41.0	32.0	41.0
29	36.97750685981939	39.0	35.0	41.0	31.0	41.0
30	36.91609729723999	39.0	35.0	41.0	31.0	41.0
31	36.862693360330894	39.0	35.0	41.0	31.0	41.0
32	36.779980458569874	39.0	35.0	41.0	31.0	41.0
33	36.693951167626956	39.0	35.0	41.0	31.0	41.0
34	36.59734582854074	39.0	35.0	41.0	30.0	41.0
35	36.49725624892884	39.0	35.0	41.0	30.0	41.0
36	36.37350987760987	39.0	35.0	41.0	30.0	41.0
37	36.31013592425134	39.0	35.0	41.0	30.0	41.0
38	36.21405145438772	39.0	35.0	41.0	30.0	41.0
39	36.14983612408278	39.0	35.0	41.0	30.0	41.0
40	36.02315217756198	38.0	35.0	41.0	29.0	41.0
41	35.964252610971826	38.0	35.0	40.0	29.0	41.0
42	35.92325730550888	38.0	35.0	40.0	29.0	41.0
43	35.84229889200444	38.0	35.0	40.0	29.0	41.0
44	35.69556148041352	38.0	35.0	40.0	28.0	41.0
45	35.65176535442253	38.0	35.0	40.0	28.0	41.0
46	35.57349291578986	38.0	34.0	40.0	28.0	41.0
47	35.50253561540487	38.0	34.0	40.0	28.0	41.0
48	35.38768925239012	38.0	34.0	40.0	27.0	41.0
49	35.286131768605436	38.0	34.0	40.0	27.0	41.0
50	35.14306765115897	38.0	34.0	40.0	27.0	41.0
51	34.78198407355776	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	7.0
12	11.0
13	10.0
14	21.0
15	52.0
16	156.0
17	323.0
18	708.0
19	1534.0
20	2777.0
21	4576.0
22	7283.0
23	10985.0
24	15948.0
25	22690.0
26	30791.0
27	36884.0
28	42152.0
29	49700.0
30	60344.0
31	73899.0
32	92291.0
33	119726.0
34	199069.0
35	294252.0
36	195644.0
37	263382.0
38	434569.0
39	869948.0
40	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.872602596925315	24.65810448127751	30.781215491088858	12.688077430708313
2	29.152280039648254	25.799493618998653	31.699804055641838	13.348422285711257
3	29.06513868938137	25.734649994611086	31.53106928373414	13.669142032273395
4	26.04526332342127	27.90901397053237	31.165436051288303	14.880286654758054
5	23.612408278074902	32.629700500197	29.368331221940114	14.389559999787977
6	22.113725469409534	41.426453725151376	26.089434729679827	10.370386075759262
7	91.21731095079836	2.915242138814828	4.568418858010132	1.2990280523766866
8	92.42280163328192	2.1198741291607255	4.28144606582953	1.1758781717278264
9	87.6872735111144	5.135509040120005	5.227138205262758	1.950079243502828
10	42.37914261533596	39.153463833335984	10.48639785715674	7.980995694171318
11	30.863869026479872	23.570003728066688	28.37026946324674	17.195857782206698
12	31.510079031480075	21.984320917634463	28.62964396079699	17.875956090088465
13	24.377916417098223	28.15531373183009	28.760956717322433	18.705813133749253
14	18.165649840894595	32.32180813001235	29.615761771238052	19.896780257855003
15	16.977403675414372	28.938667118981865	37.11673795931637	16.967191246287396
16	21.978843663258402	26.814234500695257	35.189203801567906	16.017718034478435
17	21.964991510255715	26.150779978691713	28.37595874037284	23.50826977067973
18	22.07768160190255	28.510310489648873	30.592550580677308	18.819457327771268
19	25.639699139717692	27.69077188649009	27.564901047215702	19.104627926576523
20	27.26778650015813	28.13361673707589	27.677555801737526	16.921040961028453
21	25.776135779369124	26.62344936278329	30.188894601724098	17.411520256123485
22	24.573790101011173	23.85012111799596	29.89435966479203	21.681729116200835
23	21.213900918235193	27.46210535057078	30.525763414414364	20.798230316779655
24	20.736390348017675	26.243080549209598	34.181707030497705	18.838822072275022
25	20.5594927002334	28.519180108025594	31.461349136095635	19.459978055645372
26	20.153575145279756	34.284043344517535	26.378598423610853	19.18378308659186
27	19.46881233689708	32.23943729162139	30.080586313578113	18.21116405790341
28	18.024336677992213	30.143451058965294	33.46157175998318	18.370640503059313
29	19.01119656805842	27.33605782567136	33.676668839899854	19.976076766370365
30	20.501433803847153	28.331221940114176	31.09133410014895	20.076010155889726
31	24.307206829959522	27.72617968574695	28.307616740609596	19.658996743683932
32	24.685914798657898	28.17036734708301	28.656959558427285	18.48675829583181
33	23.773192196856055	28.30797011185967	28.06693558218797	19.851902109096304
34	20.402489853827984	28.7705684153243	29.605266645111023	21.2216750857367
35	20.739040632393188	27.22008138139889	30.79061516634068	21.25026281986724
36	23.629582120828232	27.220046044273882	29.156379146149046	19.993992688748836
37	21.368854211390214	30.111188263834045	29.084927479385204	19.435030045390537
38	20.705470363636685	30.73616065670513	27.269093973783388	21.289275005874796
39	20.745825360394505	29.49229385646413	28.33528570948996	21.426595073651402
40	22.323734003325225	27.656176841108383	28.591409191539586	21.428679964026806
41	19.280076752235516	26.97925887447723	29.66512773487262	24.075536638414636
42	20.54136475510489	27.38153670555517	28.54819188765621	23.528906651683727
43	21.26121732861936	27.102691452126148	28.83297377808639	22.803117441168105
44	20.11159464077162	29.537737399222934	29.354408394687415	20.996259565318027
45	20.079932576765486	30.807364963593926	27.231353924276075	21.881348535364513
46	20.999934626318737	29.993374289061215	28.007357189426425	20.99933389519362
47	20.40432738432834	28.65929180867774	28.870749164718706	22.065631642275214
48	21.275069481622044	26.929892910842668	30.459364956526503	21.335672651008785
49	20.590377347489387	26.875791772457188	30.518625315162986	22.015205564890447
50	19.778506900457085	29.779195974394717	29.25072926991733	21.19156785523087
51	19.426690483888923	30.213135869478798	28.520063536150765	21.840110110481522
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1326.0
1	2822.0
2	4318.0
3	12391.5
4	20465.0
5	14591.0
6	8717.0
7	8141.0
8	7565.0
9	7778.5
10	7992.0
11	7827.5
12	7663.0
13	7654.5
14	7646.0
15	7450.0
16	7254.0
17	7703.0
18	8152.0
19	8281.0
20	8410.0
21	10744.0
22	13078.0
23	15141.0
24	17204.0
25	22063.0
26	34103.5
27	41285.0
28	48208.0
29	55131.0
30	65398.5
31	75666.0
32	82975.0
33	90284.0
34	98162.5
35	106041.0
36	114826.0
37	123611.0
38	137257.5
39	150904.0
40	174233.0
41	197562.0
42	215368.0
43	233174.0
44	238970.5
45	244767.0
46	239505.0
47	234243.0
48	225993.5
49	217744.0
50	209130.5
51	200517.0
52	179153.5
53	157790.0
54	144353.0
55	130916.0
56	123329.5
57	115743.0
58	106356.0
59	96969.0
60	87451.0
61	77933.0
62	65034.0
63	52135.0
64	43144.0
65	34153.0
66	26839.5
67	19526.0
68	15718.0
69	11910.0
70	9714.0
71	7518.0
72	5659.5
73	3801.0
74	2848.5
75	1391.0
76	886.0
77	657.5
78	429.0
79	390.0
80	351.0
81	241.0
82	131.0
83	102.5
84	74.0
85	54.5
86	35.0
87	32.0
88	29.0
89	20.5
90	12.0
91	8.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2829885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.87667936963043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34914146459637	15.167249810522634
2	7.304371407136282	2.757645540984157
3	2.2019487299065332	1.2469644048843176
4	1.0635307127757905	0.8030371305929245
5	0.6504033541075307	0.6138727788210031
6	0.4745590148624573	0.5374859019315774
7	0.34610349642663457	0.4573299311527529
8	0.27346617037599097	0.4129706573302648
9	0.2532122680865959	0.4301826117414818
>10	3.81823511007229	18.66820525387937
>50	2.1347014022326656	29.375119929666653
>100	1.1267457178787026	28.566205545043733
>500	0.002450261581606727	0.30452133418321686
>1k	9.424083006179721E-4	0.21411489532826256
>5k	0.0	0.0
>10k+	1.8848166012359438E-4	0.4450942739376146
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12510	0.44206743383565056	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.067425001369313E-5	0.0	0.0	0.02254508575436811	0.0
2	7.067425001369313E-5	0.0	0.0	0.06774126863812487	0.0
3	7.067425001369313E-5	0.0	0.0	0.10226563976981397	0.0
4	7.067425001369313E-5	0.0	0.0	0.15884037690577532	0.0
5	7.067425001369313E-5	0.0	0.0	0.31033063181012654	0.0
6	7.067425001369313E-5	0.0	0.0	0.44987693846216364	0.0
7	7.067425001369313E-5	0.0	0.0	0.5468420094809506	0.0
8	7.067425001369313E-5	0.0	0.0	0.752433402770784	0.0
9	7.067425001369313E-5	0.0	0.0	0.8277721532853809	0.0
10	7.067425001369313E-5	0.0	0.0	0.9687319449376918	0.0
11	7.067425001369313E-5	0.0	0.0	1.2139362553602002	0.0
12	7.067425001369313E-5	0.0	0.0	1.405816844147377	0.0
13	7.067425001369313E-5	0.0	0.0	1.4818270000371039	0.0
14	7.067425001369313E-5	0.0	0.0	1.5144785035434303	0.0
15	7.067425001369313E-5	0.0	0.0	1.5619362624276252	0.0
16	7.067425001369313E-5	0.0	0.0	1.6727181493240892	0.0
17	7.067425001369313E-5	0.0	0.0	1.8200032863526256	0.0
18	7.067425001369313E-5	0.0	0.0	1.982271364384065	0.0
19	7.067425001369313E-5	0.0	0.0	2.096728312281241	0.0
20	7.067425001369313E-5	0.0	0.0	2.2205849354302383	0.0
21	7.067425001369313E-5	0.0	0.0	2.3820755967115272	0.0
22	7.067425001369313E-5	0.0	0.0	2.569574381997855	0.0
23	7.067425001369313E-5	0.0	0.0	2.75912272053458	0.0
24	7.067425001369313E-5	0.0	0.0	2.8927323901854667	0.0
25	7.067425001369313E-5	0.0	0.0	3.008108103332821	0.0
26	7.067425001369313E-5	0.0	0.0	3.1238018506052367	0.0
27	7.067425001369313E-5	0.0	0.0	3.240944420002933	0.0
28	7.067425001369313E-5	0.0	0.0	3.3699956005279366	0.0
29	7.067425001369313E-5	0.0	0.0	3.521697878182329	0.0
30	7.067425001369313E-5	0.0	0.0	3.721882691346115	0.0
31	7.067425001369313E-5	0.0	0.0	3.8967661230049986	0.0
32	7.067425001369313E-5	0.0	0.0	4.0517194161600205	0.0
33	7.067425001369313E-5	0.0	0.0	4.205753944064865	0.0
34	7.067425001369313E-5	0.0	0.0	4.37703298897305	0.0
35	7.067425001369313E-5	0.0	0.0	4.566652001759789	0.0
36	7.067425001369313E-5	0.0	0.0	4.745139820169371	0.0
37	7.067425001369313E-5	0.0	0.0	4.9259598888294045	0.0
38	7.067425001369313E-5	0.0	0.0	5.1241304858678	0.0
39	7.067425001369313E-5	0.0	0.0	5.411209289423422	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	55	1.8189894E-12	45.0	1
AATCGGC	30	2.1666328E-6	44.999996	41
CGACGGT	380	0.0	42.039474	28
CGTAAGG	515	0.0	39.757286	2
CGTTTTT	5535	0.0	39.5122	1
TACGGGA	1710	0.0	39.473686	4
TCGCACG	40	3.4606092E-7	39.375	1
AGGGATT	6350	0.0	39.11811	6
TAGGGAC	3955	0.0	39.02655	5
CGTTAGG	515	0.0	38.8835	2
ACGGGAC	1245	0.0	38.855423	5
CACGACG	390	0.0	38.653847	26
GACCGAT	845	0.0	38.60947	9
GTCGATC	35	6.2515282E-6	38.571426	23
TTAGGGA	5090	0.0	38.36935	4
ACGGGAT	1555	0.0	37.909966	5
ATAGGGA	4405	0.0	37.849033	4
TAGGGAT	5690	0.0	37.72408	5
AAGGGAT	5045	0.0	37.552032	5
CGAAACG	30	1.14034396E-4	37.499996	42
>>END_MODULE