FastQCFastQC Report
Thu 26 May 2016
SRR1548096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548096_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2229110
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51400.2305853008599845No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC253.8919887E-545.00000445
CGTAAGC253.8919887E-545.00000427
GTCGCGC207.034714E-445.019
TATTCGC207.034714E-445.027
GTATCCG207.034714E-445.09
CGACGGT4300.042.90697528
GACGGTC4400.041.93181629
CGTTAGG4150.041.204822
CGGTCTA4550.041.04395731
GCGAATG2050.040.6097561
CCGGTAA501.0822987E-940.50000425
CGTTTTT33500.040.2985081
CTCACGA4850.039.43298724
GGCGATA3900.039.230778
AAGTACG750.039.01
CACGACG4800.038.90624626
TCTCACG5050.038.31683323
TCAACGG1650.038.1818162
TCGCGCA3150.037.85714334
CGAACGG1250.037.8000032
Produced by FastQC (version 0.11.5)