##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548096_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2229110
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.528419414026224	31.0	31.0	33.0	30.0	34.0
2	31.94401846476845	31.0	31.0	34.0	30.0	34.0
3	32.05411307651933	33.0	31.0	34.0	30.0	34.0
4	35.76741928392946	37.0	35.0	37.0	35.0	37.0
5	35.65771810274055	37.0	35.0	37.0	33.0	37.0
6	35.708941236637045	37.0	35.0	37.0	33.0	37.0
7	36.057971118518154	37.0	35.0	37.0	35.0	37.0
8	36.08381013050052	37.0	35.0	37.0	35.0	37.0
9	37.92257492900754	39.0	38.0	39.0	35.0	39.0
10	37.31745808865421	39.0	37.0	39.0	34.0	39.0
11	37.00172400644203	39.0	37.0	39.0	33.0	39.0
12	36.38459205691958	38.0	35.0	39.0	32.0	39.0
13	36.1487418745598	38.0	35.0	39.0	32.0	39.0
14	37.18267155950132	39.0	35.0	41.0	32.0	41.0
15	37.38029078870042	39.0	35.0	41.0	32.0	41.0
16	37.49887354145825	39.0	35.0	41.0	33.0	41.0
17	37.43265473664377	39.0	35.0	41.0	33.0	41.0
18	37.3708049400882	39.0	35.0	41.0	32.0	41.0
19	37.354236444141385	39.0	35.0	41.0	32.0	41.0
20	37.24060050872321	39.0	35.0	41.0	32.0	41.0
21	37.11196351907263	39.0	35.0	41.0	32.0	41.0
22	37.013076519328344	39.0	35.0	41.0	32.0	41.0
23	36.94100291147588	39.0	35.0	41.0	32.0	41.0
24	36.952236094225945	39.0	35.0	41.0	32.0	41.0
25	36.92743292165932	39.0	35.0	41.0	32.0	41.0
26	36.804908236919665	39.0	35.0	41.0	31.0	41.0
27	36.75379232070198	38.0	35.0	41.0	31.0	41.0
28	36.717559923018605	38.0	35.0	41.0	31.0	41.0
29	36.687025315036045	38.0	35.0	41.0	31.0	41.0
30	36.624935960988914	38.0	35.0	41.0	31.0	41.0
31	36.56509817819668	38.0	35.0	41.0	31.0	41.0
32	36.48182144443298	38.0	35.0	41.0	30.0	41.0
33	36.39244362099672	38.0	35.0	41.0	30.0	41.0
34	36.308774353890115	38.0	35.0	41.0	30.0	41.0
35	36.22213618888256	38.0	35.0	41.0	30.0	41.0
36	36.07905935552754	38.0	35.0	41.0	30.0	41.0
37	36.013011470945806	38.0	35.0	40.0	30.0	41.0
38	35.91918388953439	38.0	35.0	40.0	29.0	41.0
39	35.88420176662435	38.0	35.0	40.0	29.0	41.0
40	35.75318938948728	38.0	35.0	40.0	29.0	41.0
41	35.69469833251836	38.0	35.0	40.0	29.0	41.0
42	35.631571344617356	38.0	35.0	40.0	28.0	41.0
43	35.54711880526309	38.0	34.0	40.0	28.0	41.0
44	35.38728191968992	38.0	34.0	40.0	27.0	41.0
45	35.35852021658868	38.0	34.0	40.0	27.0	41.0
46	35.28540089991073	38.0	34.0	40.0	27.0	41.0
47	35.20483017886062	37.0	34.0	40.0	27.0	41.0
48	35.088465800252116	37.0	34.0	40.0	27.0	41.0
49	34.99472480048091	37.0	34.0	40.0	27.0	41.0
50	34.84626599853753	37.0	34.0	40.0	26.0	41.0
51	34.477816258506756	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	2.0
11	6.0
12	14.0
13	21.0
14	41.0
15	76.0
16	164.0
17	346.0
18	764.0
19	1453.0
20	2689.0
21	4399.0
22	6520.0
23	9797.0
24	13974.0
25	19359.0
26	25770.0
27	31033.0
28	35361.0
29	41499.0
30	50145.0
31	62218.0
32	77109.0
33	100720.0
34	174724.0
35	269061.0
36	150109.0
37	199455.0
38	325190.0
39	626973.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.270184064492106	24.033852075492014	30.807048553009945	11.888915307005934
2	30.336232846292916	25.250122246098215	31.125022991238655	13.28862191637021
3	29.647258322828396	25.462718304614846	30.699786013251924	14.190237359304836
4	26.687736361148616	28.019478625998715	30.351799597148638	14.940985415704025
5	24.313246093732477	31.34892401003091	29.64541902373593	14.692410872500684
6	22.141482475068525	41.419983760334844	26.130608179946258	10.307925584650377
7	90.70817501155169	3.415398970889727	4.426609723163056	1.449816294395521
8	91.72566629731148	2.53253540650753	4.2370273337789515	1.504770962402035
9	86.89360327664404	5.090776139356066	5.5900336905760595	2.4255868934238327
10	44.20706021685785	30.44605246039899	13.382874779620565	11.96401254312259
11	37.15249583914657	24.24371161584668	22.99550044636649	15.608292098640264
12	35.430014669531786	22.74741937365137	24.80546047525694	17.017105481559906
13	24.86768261772636	31.221698345976645	25.05143308315875	18.859185953138248
14	18.6574462453625	33.7006249130819	27.24580662237395	20.39612221918165
15	17.69562740286482	28.420939298643855	37.31251486019084	16.570918438300488
16	22.30029025036898	25.23204328184791	35.532701392035385	16.93496507574772
17	22.381802602832522	25.32477087268013	26.249444845700747	26.043981678786597
18	23.339897986191797	27.30443988856539	30.04302165438224	19.31264047086057
19	28.0787399455388	28.439825760056703	24.87373884644544	18.607695447959053
20	29.739357860311966	27.485319252975405	24.99751021708215	17.77781266963048
21	26.22786672707942	27.6376670509755	27.77359574000386	18.360870481941223
22	24.996702719919607	24.19974788144147	27.467195427771625	23.336353970867297
23	22.40849487014997	28.37800736616856	28.147197760541204	21.066300003140267
24	22.125332531817634	25.268021766534627	33.48968870984384	19.116956991803903
25	20.757432338466923	28.26769428157426	31.248839222828845	19.726034157129977
26	20.342917128360646	33.90330670088062	25.739106638972505	20.01466953178623
27	20.004037485812724	33.380407427179456	28.524792405937795	18.090762681070025
28	17.439740524245103	30.32824759657442	33.722113309796285	18.509898569384195
29	19.100089273297417	27.433639434572545	33.41046426600751	20.055807026122533
30	21.095997954340522	29.718497516946226	30.00076263620907	19.184741892504185
31	25.973729425645214	27.414842695066643	26.48666956767499	20.124758311613157
32	26.849190932704083	27.710072629883676	27.07699485444864	18.363741582963605
33	25.282108105925683	28.91916504793393	26.50878601773802	19.289940828402365
34	20.470277375275334	28.297302510867567	28.84375378514295	22.38866632871415
35	21.24148202645899	27.04272108599396	30.10044367482986	21.615353212717185
36	25.15690118477778	27.77561448291022	27.65283005324995	19.414654279062045
37	20.96105620628861	30.911843740326855	28.82423927038145	19.30286078300308
38	21.169614778992514	30.676099429817285	26.978614783478612	21.175671007711596
39	20.782150723831485	30.117042227615503	28.141724724217287	20.95908232433572
40	23.370223990740698	27.308701679145486	27.06847127328844	22.25260305682537
41	19.47405018146256	25.604748083315766	29.320715442486016	25.600486292735663
42	22.01214834620095	26.31808210451705	26.994226395287807	24.675543153994195
43	22.102408584592055	27.459569065680924	26.769293574565634	23.668728775161387
44	20.690544656836135	29.06325843049468	28.674852295310682	21.5713446173585
45	20.400249426901322	32.607722364531135	25.289869050876806	21.702159157690737
46	21.543396243343757	31.164590352203348	26.092072620911484	21.19994078354141
47	21.180201964012543	28.296360430844597	28.52219047063626	22.001247134506595
48	22.014122228153838	26.606268869638555	30.14759253693178	21.232016365275825
49	21.33977237552207	26.31094921291457	30.44219441840017	21.90708399316319
50	20.095598691854597	30.25866825773515	28.37065016979871	21.275082880611546
51	18.79104216481017	31.323846737038547	27.218441440754383	22.6666696573969
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	742.0
1	1936.5
2	3131.0
3	6566.0
4	10001.0
5	7659.5
6	5318.0
7	5252.5
8	5187.0
9	5270.5
10	5354.0
11	5302.5
12	5251.0
13	5297.0
14	5343.0
15	5215.0
16	5087.0
17	4964.0
18	4841.0
19	5447.0
20	6053.0
21	6775.5
22	7498.0
23	7896.0
24	8294.0
25	10971.5
26	17179.0
27	20709.0
28	27162.5
29	33616.0
30	38424.0
31	43232.0
32	51707.5
33	60183.0
34	67669.0
35	75155.0
36	86134.5
37	97114.0
38	101488.5
39	105863.0
40	134058.0
41	162253.0
42	176006.0
43	189759.0
44	192561.5
45	195364.0
46	193783.5
47	192203.0
48	187898.0
49	183593.0
50	175551.5
51	167510.0
52	156029.5
53	144549.0
54	127818.0
55	111087.0
56	103518.0
57	95949.0
58	87091.0
59	78233.0
60	72298.5
61	66364.0
62	57443.5
63	48523.0
64	38175.5
65	27828.0
66	23135.0
67	18442.0
68	14438.5
69	10435.0
70	8917.5
71	7400.0
72	5342.0
73	3284.0
74	2677.0
75	1681.0
76	1292.0
77	1006.5
78	721.0
79	501.5
80	282.0
81	224.5
82	167.0
83	125.5
84	84.0
85	67.5
86	51.0
87	39.5
88	28.0
89	19.5
90	11.0
91	8.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2229110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.479026299823726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52599412090831	15.06520989479539
2	7.515640420462856	2.777634339795027
3	2.1984832695491834	1.2187749047316545
4	0.9981339360240643	0.7377817301814102
5	0.5937366880126173	0.5485837936477694
6	0.3898457841544183	0.4322382298958939
7	0.30774030662082086	0.3980718853693377
8	0.22223184304196175	0.32853024577845674
9	0.19756829429944653	0.3285782735733708
>10	2.8349894133430276	13.486951702858896
>50	1.693360362325638	23.066756328515822
>100	1.5186256038737678	40.8342513659107
>500	0.0024333049225468925	0.28094198015889754
>1k	9.73321969018757E-4	0.26457468169893616
>5k	2.4333049225468926E-4	0.23112064308846567
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5140	0.2305853008599845	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.486095347470515E-5	0.0	0.0	0.028621288316861886	0.0
2	4.486095347470515E-5	0.0	0.0	0.10053339673681425	0.0
3	4.486095347470515E-5	0.0	0.0	0.14736823216440642	0.0
4	4.486095347470515E-5	0.0	0.0	0.22071589109554934	0.0
5	4.486095347470515E-5	0.0	0.0	0.3954493048795259	0.0
6	4.486095347470515E-5	0.0	0.0	0.5346977044650107	0.0
7	4.486095347470515E-5	0.0	0.0	0.6373844269686108	0.0
8	4.486095347470515E-5	0.0	0.0	0.8571582380411913	0.0
9	4.486095347470515E-5	0.0	0.0	0.9190214928828098	0.0
10	4.486095347470515E-5	0.0	0.0	1.0702029060925662	0.0
11	4.486095347470515E-5	0.0	0.0	1.329319773362463	0.0
12	4.486095347470515E-5	0.0	0.0	1.5215938199550494	0.0
13	4.486095347470515E-5	0.0	0.0	1.604137974348507	0.0
14	4.486095347470515E-5	0.0	0.0	1.635136893199528	0.0
15	4.486095347470515E-5	0.0	0.0	1.6922448869728277	0.0
16	4.486095347470515E-5	0.0	0.0	1.8351270237897637	0.0
17	4.486095347470515E-5	0.0	0.0	1.9950563229270875	0.0
18	4.486095347470515E-5	0.0	0.0	2.17118042626878	0.0
19	4.486095347470515E-5	0.0	0.0	2.282570173746473	0.0
20	4.486095347470515E-5	0.0	0.0	2.412128607381421	0.0
21	4.486095347470515E-5	0.0	0.0	2.5858302192354796	0.0
22	4.486095347470515E-5	0.0	0.0	2.768907770365751	0.0
23	4.486095347470515E-5	0.0	0.0	2.9636491693994462	0.0
24	4.486095347470515E-5	0.0	0.0	3.1060826966816353	0.0
25	4.486095347470515E-5	0.0	0.0	3.229674623504448	0.0
26	4.486095347470515E-5	0.0	0.0	3.350754336932677	0.0
27	4.486095347470515E-5	0.0	0.0	3.483273593496956	0.0
28	4.486095347470515E-5	0.0	0.0	3.6218490787803206	0.0
29	4.486095347470515E-5	0.0	0.0	3.7751838177568624	0.0
30	4.486095347470515E-5	0.0	0.0	3.955435128818228	0.0
31	4.486095347470515E-5	0.0	0.0	4.130976039764749	0.0
32	4.486095347470515E-5	0.0	0.0	4.2940456056453025	0.0
33	4.486095347470515E-5	0.0	0.0	4.450206584690751	0.0
34	4.486095347470515E-5	0.0	0.0	4.614980866803343	0.0
35	4.486095347470515E-5	0.0	0.0	4.805774501931264	0.0
36	4.486095347470515E-5	0.0	0.0	4.980642498575665	0.0
37	4.486095347470515E-5	0.0	0.0	5.16699489930959	0.0
38	4.486095347470515E-5	0.0	0.0	5.358551170646581	0.0
39	4.486095347470515E-5	0.0	0.0	5.616142765498338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAC	25	3.8919887E-5	45.000004	45
CGTAAGC	25	3.8919887E-5	45.000004	27
GTCGCGC	20	7.034714E-4	45.0	19
TATTCGC	20	7.034714E-4	45.0	27
GTATCCG	20	7.034714E-4	45.0	9
CGACGGT	430	0.0	42.906975	28
GACGGTC	440	0.0	41.931816	29
CGTTAGG	415	0.0	41.20482	2
CGGTCTA	455	0.0	41.043957	31
GCGAATG	205	0.0	40.609756	1
CCGGTAA	50	1.0822987E-9	40.500004	25
CGTTTTT	3350	0.0	40.298508	1
CTCACGA	485	0.0	39.432987	24
GGCGATA	390	0.0	39.23077	8
AAGTACG	75	0.0	39.0	1
CACGACG	480	0.0	38.906246	26
TCTCACG	505	0.0	38.316833	23
TCAACGG	165	0.0	38.181816	2
TCGCGCA	315	0.0	37.857143	34
CGAACGG	125	0.0	37.800003	2
>>END_MODULE