FastQCFastQC Report
Thu 26 May 2016
SRR1548095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548095_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1763204
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208181.1806915138577272No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA207.0343143E-445.029
CGACGGT4550.044.01098628
CGGTCTA4550.044.01098631
CGTTTTT79450.043.923851
GCTAGCG1000.042.751
TCACGAC4650.042.58064725
CGGGTCA700.041.7857136
CTCACGA4750.041.68420824
ACGGTCT4750.041.68420830
GCGCGAC650.041.538469
TCGTCCC3450.041.08695638
CGTGCGG1100.040.9090922
CGGGACG556.184564E-1140.9090926
CTCGTCC3550.040.5633837
TATTACG501.0822987E-940.51
TCGTAAG1000.040.51
TACGGGA6950.040.4676254
ACGGGAC6950.040.4676255
CTACGGG3750.040.1999973
CACGACG5000.040.05000326
Produced by FastQC (version 0.11.5)