##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548095_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1763204
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.493416530361774	31.0	31.0	33.0	30.0	34.0
2	31.88419717741112	31.0	31.0	34.0	30.0	34.0
3	31.856936576822648	33.0	31.0	34.0	30.0	34.0
4	35.6314062354668	37.0	35.0	37.0	33.0	37.0
5	35.57052558864431	37.0	35.0	37.0	33.0	37.0
6	35.64437126957516	37.0	35.0	37.0	33.0	37.0
7	36.07114037853816	37.0	35.0	37.0	35.0	37.0
8	36.119172824018094	37.0	35.0	37.0	35.0	37.0
9	37.9453381457846	39.0	38.0	39.0	35.0	39.0
10	37.33550570438815	39.0	37.0	39.0	34.0	39.0
11	36.98074584676532	39.0	37.0	39.0	33.0	39.0
12	36.535987894764304	39.0	35.0	39.0	32.0	39.0
13	36.35407984555389	39.0	35.0	39.0	32.0	39.0
14	37.40567852613764	40.0	35.0	41.0	32.0	41.0
15	37.60707722986109	40.0	35.0	41.0	32.0	41.0
16	37.708270852380096	40.0	35.0	41.0	33.0	41.0
17	37.65013690985275	40.0	35.0	41.0	33.0	41.0
18	37.52365806792634	39.0	36.0	41.0	32.0	41.0
19	37.45772185181068	39.0	36.0	41.0	32.0	41.0
20	37.29065666820175	39.0	35.0	41.0	32.0	41.0
21	37.17139707033333	39.0	35.0	41.0	32.0	41.0
22	37.1042760792285	39.0	35.0	41.0	32.0	41.0
23	37.02905165823127	39.0	35.0	41.0	32.0	41.0
24	37.022822656935894	39.0	35.0	41.0	32.0	41.0
25	37.01617566657063	39.0	35.0	41.0	32.0	41.0
26	36.89770100340063	39.0	35.0	41.0	32.0	41.0
27	36.832090898160395	39.0	35.0	41.0	31.0	41.0
28	36.777490296074646	38.0	35.0	41.0	31.0	41.0
29	36.72961608526296	38.0	35.0	41.0	31.0	41.0
30	36.62928169400705	38.0	35.0	41.0	31.0	41.0
31	36.51518088661324	38.0	35.0	41.0	30.0	41.0
32	36.36109037865159	38.0	35.0	41.0	30.0	41.0
33	36.20396335307769	38.0	35.0	41.0	30.0	41.0
34	36.04316913981592	38.0	35.0	41.0	30.0	41.0
35	35.88809973207865	38.0	35.0	41.0	29.0	41.0
36	35.728861776629365	38.0	35.0	41.0	28.0	41.0
37	35.64510345938417	38.0	35.0	40.0	27.0	41.0
38	35.533964305888595	38.0	35.0	40.0	27.0	41.0
39	35.494684109155834	38.0	35.0	40.0	27.0	41.0
40	35.39009609778562	38.0	35.0	40.0	26.0	41.0
41	35.313733975195156	38.0	34.0	40.0	26.0	41.0
42	35.28181707845491	38.0	34.0	40.0	26.0	41.0
43	35.1928767176118	38.0	34.0	40.0	26.0	41.0
44	35.03185337601321	38.0	34.0	40.0	25.0	41.0
45	35.00870971254602	38.0	34.0	40.0	25.0	41.0
46	34.92593369797255	38.0	34.0	40.0	24.0	41.0
47	34.85015290346438	38.0	34.0	40.0	24.0	41.0
48	34.724941073182684	37.0	34.0	40.0	24.0	41.0
49	34.62257458581083	37.0	34.0	40.0	24.0	41.0
50	34.46572546341773	37.0	34.0	40.0	24.0	41.0
51	34.12492485271132	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	7.0
14	18.0
15	28.0
16	100.0
17	281.0
18	566.0
19	1319.0
20	2195.0
21	3674.0
22	5666.0
23	8704.0
24	13101.0
25	19991.0
26	26668.0
27	30439.0
28	31597.0
29	35317.0
30	40654.0
31	49103.0
32	60293.0
33	77397.0
34	123953.0
35	188425.0
36	123849.0
37	161465.0
38	254519.0
39	503793.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.714369976474643	25.033405096630908	29.311866352390307	14.940358574504142
2	30.399375228277613	25.838076592385228	30.923761515967524	12.838786663369639
3	27.576275915889482	25.286693995703274	34.325977028182784	12.811053060224456
4	24.92025880159074	27.25912600016788	33.777827182787696	14.042788015453686
5	22.641622863831977	31.30165312692122	32.27936188892493	13.777362120321868
6	21.51265537056404	40.1542873087856	28.400060344690687	9.932996975959673
7	88.87496852321115	2.487914047381925	7.361768689272484	1.275348740134437
8	89.82539740154854	1.8107944401215061	7.121070505738417	1.2427376525915321
9	84.95171290446257	4.9915381317193015	8.143130346800485	1.9136186170176563
10	38.14567117588209	40.11396299010211	13.336063212197793	8.40430262181801
11	28.96698283352352	22.25766275484856	32.19020601132937	16.585148400298547
12	30.381453308862728	22.49541176177005	30.00486614141075	17.11826878795647
13	22.794072608728204	28.864782520910797	31.122717507446673	17.218427362914333
14	16.431847931379465	32.78253679097824	31.93011132007414	18.855503957568153
15	15.478016156950641	29.47225618816654	39.08980469645032	15.95992295843249
16	20.304570543170275	27.000108892674923	37.0186319903993	15.676688573755504
17	20.638848369218763	26.922182572181097	29.465904115462532	22.973064943137604
18	20.712180779989158	28.836481768417038	32.851161862155486	17.60017558943832
19	23.728224300761568	29.37504678982126	28.673823335246517	18.22290557417066
20	25.070269804288102	29.356160716513802	29.50617171921116	16.06739775998693
21	23.517188028157832	27.816690524749266	32.179827178250505	16.486294268842403
22	22.575493249788455	24.413794433315715	31.870674068343764	21.140038248552067
23	18.817448236278956	28.083080573773657	32.728487458059305	20.370983731888085
24	18.550094033361994	27.350947479701727	36.44371269575159	17.655245791184683
25	18.83270455375555	30.050975383449675	32.790647026662825	18.325673036131953
26	18.23271725790096	34.103144049128744	28.79167696987983	18.872461723090463
27	17.861121004716413	32.466407744084066	32.42336110852743	17.249110142672087
28	16.141807754519615	30.344531886270676	35.76988255471289	17.743777804496812
29	17.337755585853934	28.01955984673356	34.810662861472636	19.832021705939866
30	19.34007636098829	29.64427258558851	32.4800193284498	18.5356317249734
31	23.195727777387077	28.63037969514588	29.495112306914006	18.678780220553037
32	23.467902749766903	29.691402696454862	29.214770383914733	17.6259241698635
33	23.00488202159251	29.589145668907285	28.700876359173417	18.705095950326793
34	18.95764755524602	29.564361242374677	30.77806084831931	20.699930354059994
35	19.015156499191246	29.59271870980329	30.68085144997402	20.71127334103144
36	23.080596459626907	29.021656030725886	29.44940006941908	18.44834744022813
37	19.250466763913877	31.609048073847383	30.720551904374084	18.41993325786466
38	18.991960090834638	32.17336167567678	29.06198034940937	19.77269788407921
39	18.794251827922352	31.673022520366334	29.350772797702362	20.18195285400895
40	21.512712085498897	29.069636865615095	29.019954582680167	20.39769646620584
41	18.34512625878798	28.188570352608096	30.11302152218348	23.353281866420446
42	19.761581756847193	29.189362093098698	28.63917051004875	22.409885640005353
43	20.542943414375195	29.196394745021	28.6312871341036	21.62937470650021
44	19.778142517825504	30.88780424726804	29.35905317819152	19.975000056714933
45	18.754211083913148	32.60949952472885	27.068563819047597	21.567725572310408
46	20.67344447948167	31.677276140480625	27.892121388109377	19.75715799192833
47	19.954525965231475	29.581602582571275	29.24658746236964	21.21728398982761
48	20.194373424742686	28.806990002291283	31.086136374463756	19.912500198502272
49	20.1568281378672	28.6827275800191	30.31906688051978	20.841377401593917
50	18.434395566253254	31.02624540325453	30.205807155609897	20.333551874882318
51	17.835655998965517	31.61540014655139	28.958305448490364	21.590638405992728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	907.0
1	1609.5
2	2312.0
3	15780.0
4	29248.0
5	19945.0
6	10642.0
7	9871.5
8	9101.0
9	9746.0
10	10391.0
11	10680.0
12	10969.0
13	11045.0
14	11121.0
15	10903.5
16	10686.0
17	10259.5
18	9833.0
19	10108.5
20	10384.0
21	10941.5
22	11499.0
23	12311.0
24	13123.0
25	14770.5
26	21884.0
27	27350.0
28	33103.0
29	38856.0
30	42090.0
31	45324.0
32	52679.0
33	60034.0
34	66195.0
35	72356.0
36	76457.5
37	80559.0
38	86435.5
39	92312.0
40	108483.5
41	124655.0
42	135356.5
43	146058.0
44	146019.0
45	145980.0
46	151498.0
47	157016.0
48	150090.0
49	143164.0
50	132262.5
51	121361.0
52	108513.5
53	95666.0
54	84432.0
55	73198.0
56	66344.5
57	59491.0
58	51753.0
59	44015.0
60	38262.5
61	32510.0
62	26806.0
63	21102.0
64	16403.5
65	11705.0
66	8976.5
67	6248.0
68	4799.0
69	3350.0
70	2534.5
71	1719.0
72	1475.0
73	1231.0
74	909.0
75	505.5
76	424.0
77	289.5
78	155.0
79	105.5
80	56.0
81	46.5
82	37.0
83	26.5
84	16.0
85	15.5
86	15.0
87	12.5
88	10.0
89	7.0
90	4.0
91	4.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1763204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.854772552744052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37785983195256	19.480638792940354
2	7.717453650029739	3.8363111031566715
3	2.773322738629853	2.0679091765199473
4	1.4324337562524732	1.4241126083411215
5	0.9292696652278104	1.1548393084700919
6	0.6492813506022991	0.9682644175175168
7	0.5237611734036738	0.9112575385834654
8	0.440015212121027	0.8749182413612444
9	0.3649585593134946	0.816386578462265
>10	4.891170148759661	29.532145382740005
>50	1.6013410879670227	27.79121941132434
>100	0.2959212771881434	9.296562676901294
>500	0.0018351706012552584	0.27111077026929736
>1k	0.0011469816257845364	0.38736626145312436
>5k	0.0	0.0
>10k+	2.293963251569073E-4	1.1869577319592497
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20818	1.1806915138577272	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1342986971445165E-4	0.0	0.0	0.036070698569195626	0.0
2	1.1342986971445165E-4	0.0	0.0	0.12800560797275867	0.0
3	1.1342986971445165E-4	0.0	0.0	0.22084795633403737	0.0
4	1.1342986971445165E-4	0.0	0.0	0.33354053189534505	0.0
5	1.1342986971445165E-4	0.0	0.0	0.6844358338570012	0.0
6	1.1342986971445165E-4	0.0	0.0	1.0911386317181677	0.0
7	1.1342986971445165E-4	0.0	0.0	1.37301185795858	0.0
8	1.1342986971445165E-4	0.0	0.0	1.8313252465398218	0.0
9	1.1342986971445165E-4	0.0	0.0	2.02926036919154	0.0
10	1.1342986971445165E-4	0.0	0.0	2.2955369883462153	0.0
11	1.1342986971445165E-4	0.0	0.0	2.691804238193652	0.0
12	1.1342986971445165E-4	0.0	0.0	2.986892044255798	0.0
13	1.1342986971445165E-4	0.0	0.0	3.119037842473134	0.0
14	1.1342986971445165E-4	0.0	0.0	3.1852808863863737	0.0
15	1.1342986971445165E-4	0.0	0.0	3.2678578315384947	0.0
16	1.1342986971445165E-4	0.0	0.0	3.426659649138727	0.0
17	1.1342986971445165E-4	0.0	0.0	3.650967216499055	0.0
18	1.1342986971445165E-4	0.0	0.0	3.890587816270834	0.0
19	1.1342986971445165E-4	0.0	0.0	4.059825181884796	0.0
20	1.1342986971445165E-4	0.0	0.0	4.238930946163915	0.0
21	1.1342986971445165E-4	0.0	0.0	4.4707248849253975	0.0
22	1.1342986971445165E-4	0.0	0.0	4.739440246278933	0.0
23	1.1342986971445165E-4	0.0	0.0	5.011218214114759	0.0
24	1.1342986971445165E-4	0.0	0.0	5.207395173785903	0.0
25	1.1342986971445165E-4	0.0	0.0	5.36937302773814	0.0
26	1.7014480457167746E-4	0.0	0.0	5.533109044670951	0.0
27	1.7014480457167746E-4	0.0	0.0	5.702289695350056	0.0
28	1.7014480457167746E-4	0.0	0.0	5.885195360264609	0.0
29	1.7014480457167746E-4	0.0	0.0	6.0983300854580635	0.0
30	1.7014480457167746E-4	0.0	0.0	6.355192025426439	0.0
31	1.7014480457167746E-4	0.0	0.0	6.5779115746107655	0.0
32	1.7014480457167746E-4	0.0	0.0	6.773861674542481	0.0
33	1.7014480457167746E-4	0.0	0.0	6.970719213431911	0.0
34	1.7014480457167746E-4	0.0	0.0	7.1804510425339325	0.0
35	1.7014480457167746E-4	0.0	0.0	7.42908931694801	0.0
36	1.7014480457167746E-4	0.0	0.0	7.656289345986057	0.0
37	1.7014480457167746E-4	0.0	0.0	7.888650434096111	0.0
38	1.7014480457167746E-4	0.0	0.0	8.144491505237058	0.0
39	1.7014480457167746E-4	0.0	0.0	8.51506688959417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGTA	20	7.0343143E-4	45.0	29
CGACGGT	455	0.0	44.010986	28
CGGTCTA	455	0.0	44.010986	31
CGTTTTT	7945	0.0	43.92385	1
GCTAGCG	100	0.0	42.75	1
TCACGAC	465	0.0	42.580647	25
CGGGTCA	70	0.0	41.785713	6
CTCACGA	475	0.0	41.684208	24
ACGGTCT	475	0.0	41.684208	30
GCGCGAC	65	0.0	41.53846	9
TCGTCCC	345	0.0	41.086956	38
CGTGCGG	110	0.0	40.909092	2
CGGGACG	55	6.184564E-11	40.909092	6
CTCGTCC	355	0.0	40.56338	37
TATTACG	50	1.0822987E-9	40.5	1
TCGTAAG	100	0.0	40.5	1
TACGGGA	695	0.0	40.467625	4
ACGGGAC	695	0.0	40.467625	5
CTACGGG	375	0.0	40.199997	3
CACGACG	500	0.0	40.050003	26
>>END_MODULE