FastQCFastQC Report
Thu 26 May 2016
SRR1548094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548094_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2144021
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77930.3634759174467041No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21770.10153818456069227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG950.045.021
TGCGCGA207.034655E-445.027
CGTCGTC253.89194E-545.029
TATCGTG207.034655E-445.01
CGTTGCG302.1663855E-644.99999615
CGTTAGG1750.042.428572
TATTACG1400.041.7857131
CGGTCTA3650.041.3013731
TTTCGCG603.6379788E-1241.2499961
TCGATAG1200.041.2499961
CGACGGT3750.040.828
CGTTTTT38550.040.73931
GTACGCG1050.040.7142871
TCGTCCC3350.040.29850438
TAACGCG451.929584E-840.0000041
CGTAAGT451.929584E-840.00000430
TACGTAG850.039.7058831
TAGGGCG4600.039.6195645
CTCACGA4050.039.44444724
TACGCGG2350.039.2553182
Produced by FastQC (version 0.11.5)