##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548094_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2144021
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.478764433743887	31.0	31.0	33.0	30.0	34.0
2	31.884749263183522	31.0	31.0	34.0	30.0	34.0
3	31.982282822789514	33.0	31.0	34.0	30.0	34.0
4	35.69534813325056	37.0	35.0	37.0	33.0	37.0
5	35.58995924013804	37.0	35.0	37.0	33.0	37.0
6	35.65009391232642	37.0	35.0	37.0	33.0	37.0
7	36.043513566331676	37.0	35.0	37.0	35.0	37.0
8	36.07298389334806	37.0	35.0	37.0	35.0	37.0
9	37.8923322112983	39.0	38.0	39.0	35.0	39.0
10	37.26359769797031	39.0	37.0	39.0	34.0	39.0
11	36.929011889342505	39.0	37.0	39.0	33.0	39.0
12	36.35183050912281	38.0	35.0	39.0	32.0	39.0
13	36.1319996399289	38.0	35.0	39.0	32.0	39.0
14	37.13510828485355	39.0	35.0	41.0	32.0	41.0
15	37.344998020075366	39.0	35.0	41.0	32.0	41.0
16	37.47253641638771	39.0	35.0	41.0	32.0	41.0
17	37.42037601310808	39.0	35.0	41.0	32.0	41.0
18	37.34594530557303	39.0	35.0	41.0	32.0	41.0
19	37.30889063120184	39.0	35.0	41.0	32.0	41.0
20	37.19598921838919	39.0	35.0	41.0	32.0	41.0
21	37.06506885893375	39.0	35.0	41.0	32.0	41.0
22	36.97878332348424	39.0	35.0	41.0	32.0	41.0
23	36.89674914564736	39.0	35.0	41.0	31.0	41.0
24	36.914380036389566	39.0	35.0	41.0	32.0	41.0
25	36.89154537199029	39.0	35.0	41.0	31.0	41.0
26	36.77121259539902	38.0	35.0	41.0	31.0	41.0
27	36.696525826939194	38.0	35.0	41.0	31.0	41.0
28	36.67050136169375	38.0	35.0	41.0	31.0	41.0
29	36.61371273882112	38.0	35.0	41.0	31.0	41.0
30	36.54110897234682	38.0	35.0	40.0	31.0	41.0
31	36.47232279907706	38.0	35.0	41.0	30.0	41.0
32	36.37330930993679	38.0	35.0	41.0	30.0	41.0
33	36.29254517563027	38.0	35.0	41.0	30.0	41.0
34	36.19383765364238	38.0	35.0	41.0	30.0	41.0
35	36.09410542154205	38.0	35.0	41.0	30.0	41.0
36	35.9341172497844	38.0	35.0	40.0	29.0	41.0
37	35.876400930774466	38.0	35.0	40.0	29.0	41.0
38	35.76973966206488	38.0	35.0	40.0	29.0	41.0
39	35.725331981356526	38.0	35.0	40.0	29.0	41.0
40	35.589870155189715	38.0	35.0	40.0	28.0	41.0
41	35.53319860206593	38.0	34.0	40.0	28.0	41.0
42	35.48306336551741	38.0	34.0	40.0	27.0	41.0
43	35.39491637441984	38.0	34.0	40.0	27.0	41.0
44	35.24828768001806	38.0	34.0	40.0	27.0	41.0
45	35.20315565938953	38.0	34.0	40.0	27.0	41.0
46	35.131050955191206	38.0	34.0	40.0	27.0	41.0
47	35.05123737127575	37.0	34.0	40.0	26.0	41.0
48	34.91827225572884	37.0	34.0	40.0	26.0	41.0
49	34.82505815008342	37.0	34.0	40.0	26.0	41.0
50	34.67013196232686	37.0	34.0	40.0	25.0	41.0
51	34.29704653079424	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	10.0
13	11.0
14	23.0
15	49.0
16	155.0
17	346.0
18	797.0
19	1501.0
20	2637.0
21	4500.0
22	6827.0
23	9928.0
24	14444.0
25	20285.0
26	26678.0
27	32171.0
28	36197.0
29	42215.0
30	49970.0
31	61174.0
32	76522.0
33	99296.0
34	164713.0
35	250393.0
36	149716.0
37	194256.0
38	308574.0
39	590540.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.66679757334467	24.438659882529134	30.514206717191673	12.380335826934532
2	29.326018728361337	25.681091743037964	31.576323179670347	13.41656634893035
3	29.539076343002236	24.692901795271595	31.809996264029127	13.958025597697038
4	26.48943270611622	27.812600716131048	31.06252224208625	14.63544433566649
5	23.774627207476044	32.26335003248569	29.637396275502898	14.324626484535367
6	22.550945163316964	40.98625899653035	26.08080797716067	10.381987862992013
7	90.09137503783778	3.4809826955985974	5.025277271071505	1.402364995492115
8	90.81086425925865	2.6824830540372506	4.876304849626006	1.6303478370780884
9	85.99677894945992	5.589124360255799	6.030211457816878	2.383885232467406
10	42.8930500214317	35.42633211148585	12.45048439357637	9.23013347350609
11	34.78137574212193	23.236899265445626	26.0797818678082	15.901943124624246
12	33.11105628163157	21.781083300956475	27.718338579706074	17.389521837705882
13	23.784795018332378	30.86569581174811	27.95863473352173	17.390874436397777
14	18.3491672889398	33.89668291495279	29.246775101549844	18.507374694557562
15	16.94447022673752	28.385915996158616	38.416321481925785	16.253292295178078
16	21.38477188423061	26.071386427651593	36.124086471167956	16.419755216949834
17	21.161313252062364	26.116441956492032	27.56246324079848	25.159781550647125
18	22.15239496254934	27.35565556494083	31.73882158803482	18.753127884475013
19	25.985473090049027	28.7644104232188	26.469656780414	18.780459706318176
20	28.350888354171904	28.090256578643586	26.375627850660045	17.183227216524465
21	24.64448809036852	28.096833006766257	30.308611716023304	16.95006718684192
22	24.21594751170814	23.418707186170284	29.823075426966433	22.542269875155142
23	20.581514826580523	28.27999352618281	29.08152485446738	22.056966792769288
24	20.326713217827624	26.31807244425311	34.700919440621156	18.654294897298115
25	19.649294479858174	29.180077993639053	32.25742658304186	18.913200943460907
26	19.568978102360006	34.08539375313954	26.859998106361832	19.48563003813862
27	18.157751253369252	33.958062910764404	29.90586379517738	17.97832204068897
28	17.34731143025185	30.159872501248824	34.348264312709624	18.144551755789706
29	18.104067077701195	27.687928429805492	34.093322779954114	20.114681712539195
30	19.79280986520188	30.06896854088649	30.73155533457928	19.406666259332347
31	24.605729141645536	28.48045798058881	27.81376674948613	19.100046128279526
32	25.474050860509294	27.891098081595285	28.045387615139965	18.58946344275546
33	24.062963935521154	28.466698786998823	27.63746250619747	19.832874771282558
34	19.749899837734798	28.545522641802485	29.616267751108783	22.088309769353938
35	20.304278736075812	27.8787847693656	30.408424171218474	21.40851232334012
36	25.186973448487677	27.189705697845312	28.109519449669566	19.51380140399744
37	19.992807906265845	30.593310420000552	29.7905197756925	19.62336189804111
38	19.63600169961022	31.753793456314096	27.100061053506476	21.510143790569217
39	20.239587205535766	30.606043504238066	28.82392476566228	20.33044452456389
40	23.061947620848862	27.56311621947733	27.987272512722587	21.387663646951218
41	19.095148788188176	26.543583295126304	29.037635358982023	25.32363255770349
42	20.551337883350957	26.793254357116837	28.006628666416983	24.648779093115223
43	20.688090275235176	27.23802612007998	28.54542935913408	23.52845424555077
44	19.978349092662807	29.290338107695774	29.31356549212904	21.41774730751238
45	19.054477544762854	32.539560013637924	26.696426947310687	21.709535494288534
46	21.295547011899604	31.06298865542828	27.238772381427236	20.40269195124488
47	20.762249996618504	28.2254698064991	28.416885842069643	22.595394354812758
48	21.011827775940628	27.453509084099455	30.88407249742423	20.650590642535686
49	21.103991052326446	26.22632893987512	31.22282850774316	21.44685150005527
50	20.107265740400866	30.153529279797166	28.774298386069912	20.964906593732056
51	18.972668644570177	31.255104311011877	27.576875413067313	22.195351631350626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	993.0
1	2200.5
2	3408.0
3	8227.5
4	13047.0
5	9653.5
6	6260.0
7	6140.0
8	6020.0
9	6513.0
10	7006.0
11	7244.0
12	7482.0
13	7214.5
14	6947.0
15	6563.0
16	6179.0
17	6436.0
18	6693.0
19	6887.0
20	7081.0
21	8322.5
22	9564.0
23	11583.0
24	13602.0
25	14842.5
26	20958.5
27	25834.0
28	32391.5
29	38949.0
30	45009.0
31	51069.0
32	56991.5
33	62914.0
34	74164.0
35	85414.0
36	88228.0
37	91042.0
38	104161.5
39	117281.0
40	133216.5
41	149152.0
42	168903.5
43	188655.0
44	195170.0
45	201685.0
46	195652.0
47	189619.0
48	182667.5
49	175716.0
50	163494.0
51	151272.0
52	134949.0
53	118626.0
54	108610.5
55	98595.0
56	89346.5
57	80098.0
58	73355.5
59	66613.0
60	57659.5
61	48706.0
62	40629.5
63	32553.0
64	27751.0
65	22949.0
66	18660.0
67	14371.0
68	11936.0
69	9501.0
70	7874.5
71	6248.0
72	4666.5
73	3085.0
74	2544.5
75	1446.5
76	889.0
77	588.0
78	287.0
79	296.0
80	305.0
81	197.5
82	90.0
83	84.0
84	78.0
85	51.0
86	24.0
87	19.5
88	15.0
89	11.0
90	7.0
91	6.5
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2144021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.069970091997142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31745236795123	16.922863192581374
2	8.094960823207657	3.4112116488174777
3	2.589188760928493	1.6366238926589547
4	1.215842369284113	1.0247104942959682
5	0.7474181243766228	0.7874038763416021
6	0.496694532009431	0.6279203360578338
7	0.34929576293615466	0.5151755894828272
8	0.28250982322368173	0.4761978820814699
9	0.21981683059084664	0.41683806416400676
>10	2.9934008432629198	15.500989648892874
>50	1.5339923763190444	23.70606444954579
>100	1.1556514730267364	34.01419937133583
>500	0.0028874627928251015	0.3920355753919045
>1k	6.663375675750235E-4	0.20306104352697546
>5k	2.221125225250078E-4	0.3647049348250104
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7793	0.3634759174467041	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2177	0.10153818456069227	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.664133420334969E-5	0.0	0.0	0.042723462130268314	0.0
2	4.664133420334969E-5	0.0	0.0	0.13344085715578347	0.0
3	4.664133420334969E-5	0.0	0.0	0.19999804106396346	0.0
4	4.664133420334969E-5	0.0	0.0	0.30941861110502183	0.0
5	4.664133420334969E-5	0.0	0.0	0.5657593838866317	0.0
6	4.664133420334969E-5	0.0	0.0	0.7969604775326361	0.0
7	4.664133420334969E-5	0.0	0.0	0.948731379030336	0.0
8	4.664133420334969E-5	0.0	0.0	1.2802579825477456	0.0
9	4.664133420334969E-5	0.0	0.0	1.3801637204113206	0.0
10	4.664133420334969E-5	0.0	0.0	1.5935478243916454	0.0
11	4.664133420334969E-5	0.0	0.0	1.9344493360839283	0.0
12	4.664133420334969E-5	0.0	0.0	2.1957807316252964	0.0
13	4.664133420334969E-5	0.0	0.0	2.3084195537263863	0.0
14	4.664133420334969E-5	0.0	0.0	2.347644915791403	0.0
15	4.664133420334969E-5	0.0	0.0	2.424976247900557	0.0
16	4.664133420334969E-5	0.0	0.0	2.598761859142238	0.0
17	4.664133420334969E-5	0.0	0.0	2.813265355143443	0.0
18	4.664133420334969E-5	0.0	0.0	3.0554271623272347	0.0
19	4.664133420334969E-5	0.0	0.0	3.2201643547334657	0.0
20	4.664133420334969E-5	0.0	0.0	3.390125376570472	0.0
21	4.664133420334969E-5	0.0	0.0	3.618901120837902	0.0
22	4.664133420334969E-5	0.0	0.0	3.86964493351511	0.0
23	4.664133420334969E-5	0.0	0.0	4.1468343826856175	0.0
24	4.664133420334969E-5	0.0	0.0	4.347018989086394	0.0
25	4.664133420334969E-5	0.0	0.0	4.520618034991262	0.0
26	4.664133420334969E-5	0.0	0.0	4.682696671347902	0.0
27	4.664133420334969E-5	0.0	0.0	4.859327403975987	0.0
28	4.664133420334969E-5	0.0	0.0	5.049390840854637	0.0
29	4.664133420334969E-5	0.0	0.0	5.2643141088636725	0.0
30	4.664133420334969E-5	0.0	0.0	5.524899243057787	0.0
31	4.664133420334969E-5	0.0	0.0	5.754421248672471	0.0
32	4.664133420334969E-5	0.0	0.0	5.965146796603205	0.0
33	4.664133420334969E-5	0.0	0.0	6.17867082458614	0.0
34	4.664133420334969E-5	0.0	0.0	6.418593847728171	0.0
35	4.664133420334969E-5	0.0	0.0	6.678992416585472	0.0
36	4.664133420334969E-5	0.0	0.0	6.906135714155785	0.0
37	4.664133420334969E-5	0.0	0.0	7.136590546454536	0.0
38	4.664133420334969E-5	0.0	0.0	7.404171880779153	0.0
39	4.664133420334969E-5	0.0	0.0	7.7680209288994835	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	95	0.0	45.0	21
TGCGCGA	20	7.034655E-4	45.0	27
CGTCGTC	25	3.89194E-5	45.0	29
TATCGTG	20	7.034655E-4	45.0	1
CGTTGCG	30	2.1663855E-6	44.999996	15
CGTTAGG	175	0.0	42.42857	2
TATTACG	140	0.0	41.785713	1
CGGTCTA	365	0.0	41.30137	31
TTTCGCG	60	3.6379788E-12	41.249996	1
TCGATAG	120	0.0	41.249996	1
CGACGGT	375	0.0	40.8	28
CGTTTTT	3855	0.0	40.7393	1
GTACGCG	105	0.0	40.714287	1
TCGTCCC	335	0.0	40.298504	38
TAACGCG	45	1.929584E-8	40.000004	1
CGTAAGT	45	1.929584E-8	40.000004	30
TACGTAG	85	0.0	39.705883	1
TAGGGCG	460	0.0	39.619564	5
CTCACGA	405	0.0	39.444447	24
TACGCGG	235	0.0	39.255318	2
>>END_MODULE