FastQCFastQC Report
Thu 26 May 2016
SRR1548093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548093_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2302116
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83730.36370886610405384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGATA302.1664546E-645.00000431
ACCGCGT406.8193913E-945.042
CGACGGT6900.042.06521628
TCACGAC7150.041.22377825
CGTTTTT43050.041.1324041
CGGTCTA7150.040.59440631
ACGGGAT9850.040.4314735
ACGGGAC6200.039.556455
TAATACG403.4602454E-739.3751
CGTAAGG3300.038.181822
CTCACGA7800.037.7884624
TACGGGA10250.037.7560964
ATAGGGA31900.037.6645744
AGGGATC24800.037.6512076
GCGGGAT14900.037.6006745
CGTAACG301.1402776E-437.50000435
GCGATAC900.037.59
GGGCGAT24600.037.3170747
CACGACG7900.037.31012726
TAGGGAC23600.037.2775425
Produced by FastQC (version 0.11.5)