##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548093_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2302116
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.553794422175077	31.0	31.0	33.0	30.0	34.0
2	31.97517675043308	31.0	31.0	34.0	30.0	34.0
3	32.084838035963436	33.0	31.0	34.0	30.0	34.0
4	35.79125899824336	37.0	35.0	37.0	35.0	37.0
5	35.701340418988444	37.0	35.0	37.0	33.0	37.0
6	35.74650625772116	37.0	35.0	37.0	33.0	37.0
7	36.08340891597122	37.0	35.0	37.0	35.0	37.0
8	36.10749892707405	37.0	35.0	37.0	35.0	37.0
9	37.94161545291375	39.0	38.0	39.0	35.0	39.0
10	37.366958485150185	39.0	37.0	39.0	34.0	39.0
11	37.057123533305884	39.0	37.0	39.0	33.0	39.0
12	36.510601116538005	39.0	35.0	39.0	33.0	39.0
13	36.2914566424976	39.0	35.0	39.0	32.0	39.0
14	37.29535305779552	39.0	35.0	41.0	32.0	41.0
15	37.49951262230053	40.0	35.0	41.0	32.0	41.0
16	37.610802843992225	40.0	35.0	41.0	33.0	41.0
17	37.547879863568994	39.0	35.0	41.0	33.0	41.0
18	37.4680954391525	39.0	36.0	41.0	32.0	41.0
19	37.437292907916024	39.0	35.0	41.0	32.0	41.0
20	37.32521080605843	39.0	35.0	41.0	32.0	41.0
21	37.20481722033121	39.0	35.0	41.0	32.0	41.0
22	37.143533601260756	39.0	35.0	41.0	32.0	41.0
23	37.057216056879845	39.0	35.0	41.0	32.0	41.0
24	37.08077698951747	39.0	35.0	41.0	32.0	41.0
25	37.06747487963248	39.0	35.0	41.0	32.0	41.0
26	36.950962071416036	39.0	35.0	41.0	32.0	41.0
27	36.89356748313291	39.0	35.0	41.0	31.0	41.0
28	36.848103657678415	39.0	35.0	41.0	31.0	41.0
29	36.79256649100219	39.0	35.0	41.0	31.0	41.0
30	36.71160097927298	38.0	35.0	41.0	31.0	41.0
31	36.65835865786085	39.0	35.0	41.0	31.0	41.0
32	36.566721659551476	39.0	35.0	41.0	31.0	41.0
33	36.46688959201013	39.0	35.0	41.0	30.0	41.0
34	36.332413744572385	38.0	35.0	41.0	30.0	41.0
35	36.22614499008738	38.0	35.0	41.0	30.0	41.0
36	36.080020728755635	38.0	35.0	41.0	30.0	41.0
37	36.022598339962016	38.0	35.0	41.0	30.0	41.0
38	35.91234889988167	38.0	35.0	40.0	29.0	41.0
39	35.85720311226715	38.0	35.0	40.0	29.0	41.0
40	35.720505395905334	38.0	35.0	40.0	28.0	41.0
41	35.645450098952445	38.0	35.0	40.0	28.0	41.0
42	35.621373119338905	38.0	35.0	40.0	28.0	41.0
43	35.53079384357695	38.0	34.0	40.0	28.0	41.0
44	35.367295566339834	38.0	34.0	40.0	27.0	41.0
45	35.32735492042973	38.0	34.0	40.0	27.0	41.0
46	35.26083003636654	38.0	34.0	40.0	27.0	41.0
47	35.170656908687484	38.0	34.0	40.0	27.0	41.0
48	35.0455611272412	37.0	34.0	40.0	26.0	41.0
49	34.9613103770618	37.0	34.0	40.0	26.0	41.0
50	34.80851616512808	37.0	34.0	40.0	26.0	41.0
51	34.42305470271698	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	6.0
12	9.0
13	12.0
14	36.0
15	78.0
16	175.0
17	358.0
18	801.0
19	1501.0
20	2708.0
21	4526.0
22	6797.0
23	10184.0
24	14375.0
25	20466.0
26	26927.0
27	32465.0
28	36217.0
29	42187.0
30	50620.0
31	62266.0
32	78817.0
33	103026.0
34	175645.0
35	257085.0
36	159427.0
37	211935.0
38	344538.0
39	658820.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.888923060349693	24.490468768732764	30.29143622649771	13.32917194441983
2	28.78629921341931	25.844179876252976	32.258409220039304	13.111111690288412
3	29.864524637333652	25.707349238700395	31.157248374973285	13.270877748992666
4	27.16813574989271	26.986737418965856	30.702883781703445	15.142243049437996
5	24.091835511329577	32.221660420239466	28.956273272067957	14.730230796362997
6	22.071085905314934	40.65768188918369	26.25910249526957	11.012129710231804
7	90.16808883653125	3.350439334942288	4.960219207025189	1.5212526215012623
8	91.01735099360762	2.4466186760354387	4.852752858674368	1.683277471682574
9	86.08532324174803	5.0681199383523685	6.293557752954239	2.552999066945367
10	43.20351363701916	32.92401425471175	13.246074481042658	10.62639762722643
11	35.41715534751507	22.724441340054106	25.333171742866128	16.525231569564696
12	34.32437809389275	21.900764340285196	26.329689728927647	17.445167836894406
13	25.30971506214283	28.418029326063497	26.85907226221442	19.413183349579256
14	18.436169159156186	32.23047839465952	28.962137442248782	20.37121500393551
15	18.600452800814555	27.618417143184793	36.511018558578286	17.270111497422374
16	23.218117592684294	24.87567959216651	34.68847790467552	17.21772491047367
17	23.36024770254844	24.914947813229222	27.272908923790112	24.45189556043223
18	23.949618524870164	27.223041758104284	29.736338221010584	19.091001496014968
19	27.173261469013728	27.93742800102167	25.69831407279216	19.190996457172446
20	29.013611824947134	27.98890238372002	25.52034736737853	17.47713842395431
21	26.17626566167821	27.021140550693364	28.7037664479114	18.098827339717026
22	24.814822537178838	23.48157086784506	29.45338115021137	22.25022544476473
23	22.10674874767388	27.339499834065702	29.059482667250474	21.49426875100994
24	22.392138363140692	25.90217000359669	32.3973248958784	19.308366737384215
25	21.345405705012258	27.659813840831653	30.713743356112378	20.281037098043715
26	20.37160594861423	32.73158259618542	25.875759518634162	21.021051936566185
27	19.029362551669855	32.2620580370407	29.51866891155789	19.189910499731553
28	17.91121733222826	29.196182989910152	33.40778657548099	19.484813102380592
29	19.73050011380834	27.3538344722855	32.85208043382696	20.063584980079195
30	21.137727204015782	28.585527401746912	30.706966981681198	19.5697784125561
31	25.11871686743848	27.29658279600159	27.393189569943477	20.191510766616453
32	27.143940618109603	26.906854389613727	27.135383273475362	18.81382171880131
33	25.780108387240258	27.92913128617324	26.403013575336775	19.88774675124972
34	20.990992634602254	27.119267665052497	29.83598567578697	22.053754024558277
35	21.819360970515824	27.10853840553647	29.969037181445245	21.103063442502464
36	25.444547538004166	27.262961553631527	28.102667285228023	19.189823623136277
37	21.83243589810418	29.880292739375424	28.738039264746	19.549232097774397
38	21.277120701128872	29.98784596432152	26.595358357267834	22.13967497728177
39	21.27838041176031	29.092104828774918	27.969398588081575	21.660116171383198
40	23.764223870560823	26.643574867643505	27.756594367964084	21.83560689383159
41	19.820721457997774	25.74505368104822	29.644379344915723	24.78984551603829
42	21.93647062094178	26.490194238691707	27.141725264930177	24.43160987543634
43	21.9784320164579	26.80785850930188	27.897855711875508	23.31585376236471
44	21.22873043756266	28.45625502798295	28.338841309473544	21.976173224980844
45	20.76689445709947	30.681946522243013	26.35966215429631	22.191496866361206
46	22.13446238156548	29.296786087234526	27.355572004190925	21.213179527009064
47	21.299491424411283	27.66650333866756	28.708284030865517	22.325721206055647
48	21.681965635093974	26.68840319080359	30.24573913738491	21.383892036717526
49	22.207873104569885	25.55153606508099	30.086407461657018	22.154183368692106
50	20.331078016920085	29.056615739606517	28.970651348585392	21.641654894888006
51	19.502709681006518	30.1312357848171	27.237984532490977	23.128070001685405
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1151.0
1	2553.5
2	3956.0
3	9412.5
4	14869.0
5	10905.0
6	6941.0
7	6633.0
8	6325.0
9	6413.0
10	6501.0
11	6357.5
12	6214.0
13	6123.0
14	6032.0
15	5780.5
16	5529.0
17	5804.0
18	6079.0
19	6348.0
20	6617.0
21	6841.5
22	7066.0
23	8769.5
24	10473.0
25	13842.5
26	21224.5
27	25237.0
28	29871.0
29	34505.0
30	42317.5
31	50130.0
32	56522.5
33	62915.0
34	68792.5
35	74670.0
36	82247.5
37	89825.0
38	96645.5
39	103466.0
40	123373.5
41	143281.0
42	166552.5
43	189824.0
44	189289.5
45	188755.0
46	189092.5
47	189430.0
48	190546.5
49	191663.0
50	180968.5
51	170274.0
52	156016.0
53	141758.0
54	132212.5
55	122667.0
56	113784.5
57	104902.0
58	99468.0
59	94034.0
60	83480.0
61	72926.0
62	63508.5
63	54091.0
64	45409.5
65	36728.0
66	29097.0
67	21466.0
68	17606.0
69	13746.0
70	11433.0
71	9120.0
72	7374.0
73	5628.0
74	4316.0
75	2215.0
76	1426.0
77	1153.0
78	880.0
79	591.0
80	302.0
81	283.0
82	264.0
83	180.0
84	96.0
85	77.0
86	58.0
87	49.0
88	40.0
89	31.0
90	22.0
91	15.0
92	8.0
93	6.5
94	5.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2302116.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.843521969029513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30519427807528	15.935378868841699
2	7.358502653461346	2.9203721812624437
3	2.2600930342048224	1.345446173288819
4	1.0533079407631123	0.8360535705074421
5	0.6772385228530822	0.6719398753254117
6	0.4625454292836291	0.5507118232598329
7	0.3574074631946179	0.49645559934582556
8	0.29756959856402293	0.47238630931363856
9	0.2524031119483393	0.45077100272984577
>10	3.9039564698226212	19.859447975720954
>50	2.096257142936967	30.17890690693178
>100	0.9720041950844212	25.34597700265988
>500	0.002420109867815408	0.3250318020798463
>1k	8.800399519328756E-4	0.24557473293473941
>5k	2.200099879832189E-4	0.3655461757977908
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8373	0.36370886610405384	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045783965708070314	0.0
2	0.0	0.0	0.0	0.1525118629990843	0.0
3	0.0	0.0	0.0	0.22739948812310065	0.0
4	0.0	0.0	0.0	0.3309563896867056	0.0
5	0.0	0.0	0.0	0.5692154522187414	0.0
6	0.0	0.0	0.0	0.7724632468563704	0.0
7	0.0	0.0	0.0	0.9051672461335571	0.0
8	0.0	0.0	0.0	1.1872555509800549	0.0
9	4.3438297635740334E-5	0.0	0.0	1.2848179674699276	0.0
10	4.3438297635740334E-5	0.0	0.0	1.4839391238321613	0.0
11	4.3438297635740334E-5	0.0	0.0	1.7897013008901377	0.0
12	4.3438297635740334E-5	0.0	0.0	2.0313051123401253	0.0
13	4.3438297635740334E-5	0.0	0.0	2.134297316034466	0.0
14	4.3438297635740334E-5	0.0	0.0	2.184077605125024	0.0
15	4.3438297635740334E-5	0.0	0.0	2.2576620813199684	0.0
16	4.3438297635740334E-5	0.0	0.0	2.4180362761911214	0.0
17	4.3438297635740334E-5	0.0	0.0	2.6067322411207776	0.0
18	4.3438297635740334E-5	0.0	0.0	2.808850640019877	0.0
19	4.3438297635740334E-5	0.0	0.0	2.940381805260899	0.0
20	4.3438297635740334E-5	0.0	0.0	3.086986059781523	0.0
21	4.3438297635740334E-5	0.0	0.0	3.2765507906638938	0.0
22	4.3438297635740334E-5	0.0	0.0	3.4805370363613304	0.0
23	4.3438297635740334E-5	0.0	0.0	3.688649920334162	0.0
24	4.3438297635740334E-5	0.0	0.0	3.8434640131079405	0.0
25	4.3438297635740334E-5	0.0	0.0	3.9851597399957255	0.0
26	4.3438297635740334E-5	0.0	0.0	4.116647466939112	0.0
27	4.3438297635740334E-5	0.0	0.0	4.264815500174622	0.0
28	4.3438297635740334E-5	0.0	0.0	4.415285763184826	0.0
29	4.3438297635740334E-5	0.0	0.0	4.586172026083829	0.0
30	4.3438297635740334E-5	0.0	0.0	4.779038067586516	0.0
31	4.3438297635740334E-5	0.0	0.0	4.956309760237972	0.0
32	4.3438297635740334E-5	0.0	0.0	5.115988942346954	0.0
33	4.3438297635740334E-5	0.0	0.0	5.2818363627202105	0.0
34	4.3438297635740334E-5	0.0	0.0	5.462452804289619	0.0
35	4.3438297635740334E-5	0.0	0.0	5.663398369152554	0.0
36	4.3438297635740334E-5	0.0	0.0	5.848402078783172	0.0
37	4.3438297635740334E-5	0.0	0.0	6.0404862309284155	0.0
38	4.3438297635740334E-5	0.0	0.0	6.253637957426993	0.0
39	4.3438297635740334E-5	0.0	0.0	6.595931742796627	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGATA	30	2.1664546E-6	45.000004	31
ACCGCGT	40	6.8193913E-9	45.0	42
CGACGGT	690	0.0	42.065216	28
TCACGAC	715	0.0	41.223778	25
CGTTTTT	4305	0.0	41.132404	1
CGGTCTA	715	0.0	40.594406	31
ACGGGAT	985	0.0	40.431473	5
ACGGGAC	620	0.0	39.55645	5
TAATACG	40	3.4602454E-7	39.375	1
CGTAAGG	330	0.0	38.18182	2
CTCACGA	780	0.0	37.78846	24
TACGGGA	1025	0.0	37.756096	4
ATAGGGA	3190	0.0	37.664574	4
AGGGATC	2480	0.0	37.651207	6
GCGGGAT	1490	0.0	37.600674	5
CGTAACG	30	1.1402776E-4	37.500004	35
GCGATAC	90	0.0	37.5	9
GGGCGAT	2460	0.0	37.317074	7
CACGACG	790	0.0	37.310127	26
TAGGGAC	2360	0.0	37.277542	5
>>END_MODULE