FastQCFastQC Report
Thu 26 May 2016
SRR1548092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548092_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1332583
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55300.4149835319826233No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20320.15248581139035994No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13930.10453382641081269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACC351.21212E-745.00000427
TACGAAC351.21212E-745.00000441
AGCGCGC351.21212E-745.00000436
CCGCGTG351.21212E-745.00000433
CTTCGGT406.8157533E-945.034
AACCGAT207.033692E-445.026
CGCGACT253.8911414E-545.043
AATATCG253.8911414E-545.01
TAGCACG207.033692E-445.01
TCGCTAG253.8911414E-545.01
TTGCGAG900.045.01
CCGGTCA253.8911414E-545.031
GCGGACA207.033692E-445.014
CGCGTCC207.033692E-445.017
TATCGCG253.8911414E-545.01
ATACCGG406.8157533E-945.02
ATCGCAA253.8911414E-545.025
AATCCGG600.044.9999962
CGTGCGA302.1657634E-644.99999639
CTATCCG302.1657634E-644.9999961