##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548092_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1332583
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5163205593948	31.0	31.0	33.0	30.0	34.0
2	31.91788503980615	31.0	31.0	34.0	30.0	34.0
3	32.00918591937613	33.0	31.0	34.0	30.0	34.0
4	35.716307351962314	37.0	35.0	37.0	33.0	37.0
5	35.61732514972801	37.0	35.0	37.0	33.0	37.0
6	35.67593388179198	37.0	35.0	37.0	33.0	37.0
7	36.077460841088325	37.0	35.0	37.0	35.0	37.0
8	36.108699420598946	37.0	35.0	37.0	35.0	37.0
9	37.923740585014215	39.0	38.0	39.0	35.0	39.0
10	37.2993397034181	39.0	37.0	39.0	34.0	39.0
11	36.96751271778193	39.0	37.0	39.0	33.0	39.0
12	36.513276846545395	39.0	35.0	39.0	32.0	39.0
13	36.35213416350051	39.0	35.0	39.0	32.0	39.0
14	37.430214102986454	40.0	35.0	41.0	32.0	41.0
15	37.58594474040266	40.0	35.0	41.0	32.0	41.0
16	37.67884326904966	40.0	35.0	41.0	33.0	41.0
17	37.61009257959917	39.0	35.0	41.0	33.0	41.0
18	37.5550220886804	39.0	36.0	41.0	33.0	41.0
19	37.50799537439694	39.0	36.0	41.0	32.0	41.0
20	37.41735036391729	39.0	35.0	41.0	32.0	41.0
21	37.28409787607976	39.0	35.0	41.0	32.0	41.0
22	37.19969863040426	39.0	35.0	41.0	32.0	41.0
23	37.09949474066531	39.0	35.0	41.0	32.0	41.0
24	37.128351479795256	39.0	35.0	41.0	32.0	41.0
25	37.10833546578337	39.0	35.0	41.0	32.0	41.0
26	36.978426109293004	39.0	35.0	41.0	32.0	41.0
27	36.920695371320214	39.0	35.0	41.0	31.0	41.0
28	36.89218682813753	39.0	35.0	41.0	31.0	41.0
29	36.82832889208402	39.0	35.0	41.0	31.0	41.0
30	36.748447939077714	39.0	35.0	41.0	31.0	41.0
31	36.692603012345195	39.0	35.0	41.0	31.0	41.0
32	36.61409383130356	39.0	35.0	41.0	30.0	41.0
33	36.545941228426294	39.0	35.0	41.0	30.0	41.0
34	36.450042511423305	39.0	35.0	41.0	30.0	41.0
35	36.3380179696124	39.0	35.0	41.0	30.0	41.0
36	36.20578380483617	38.0	35.0	41.0	30.0	41.0
37	36.13140644897916	38.0	35.0	41.0	30.0	41.0
38	36.02993134386376	38.0	35.0	41.0	29.0	41.0
39	35.99080957809007	38.0	35.0	41.0	29.0	41.0
40	35.884412453107984	38.0	35.0	40.0	29.0	41.0
41	35.81608875394628	38.0	35.0	40.0	29.0	41.0
42	35.78139598058807	38.0	35.0	40.0	29.0	41.0
43	35.66828857939806	38.0	35.0	40.0	28.0	41.0
44	35.527856801415	38.0	34.0	40.0	27.0	41.0
45	35.50231467758481	38.0	34.0	40.0	27.0	41.0
46	35.43102605991522	38.0	34.0	40.0	27.0	41.0
47	35.36845734937336	38.0	34.0	40.0	27.0	41.0
48	35.24626458539543	38.0	34.0	40.0	27.0	41.0
49	35.141959637786165	38.0	34.0	40.0	27.0	41.0
50	34.97902119417702	37.0	34.0	40.0	26.0	41.0
51	34.60938568179243	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	4.0
13	7.0
14	13.0
15	23.0
16	69.0
17	190.0
18	417.0
19	828.0
20	1533.0
21	2436.0
22	3760.0
23	5588.0
24	8135.0
25	11641.0
26	15280.0
27	18150.0
28	20668.0
29	24517.0
30	29495.0
31	36610.0
32	45611.0
33	58295.0
34	94473.0
35	145713.0
36	92782.0
37	122260.0
38	198034.0
39	395989.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.98422462240626	23.955731087669584	29.94890374558283	12.111140544341328
2	29.976444243998312	25.9191359937805	30.436378071759883	13.668041690461308
3	29.681678364499618	26.27438591067123	31.18462414723886	12.859311577590288
4	26.50311462775677	28.137009101872078	30.392778536121202	14.967097734249949
5	23.759645740640543	32.26928453987482	28.98603689226112	14.98503282722352
6	21.41089898340291	41.85397832630313	26.077325014651997	10.657797675641968
7	89.99484459879797	3.718717708390397	4.890877341223774	1.395560351587856
8	91.23731880115535	2.574323700662548	4.6758813522309675	1.512476145951134
9	85.90339213392336	5.780502978050898	5.910926373816865	2.4051785142088713
10	41.365828620055936	35.22729916260376	13.078960184844021	10.327912032496288
11	31.217792812905465	25.13539494350446	26.987587264733225	16.65922497885685
12	29.98387342476979	25.110781092059558	28.001407792235078	16.903937690935575
13	22.974253761304176	32.19416726762986	27.67069668455924	17.16088228650673
14	17.76774880063756	35.64753565068742	28.988813454771673	17.595902093903344
15	16.6418902237234	29.048847238783626	37.109208206918446	17.20005433057453
16	20.66805594848501	25.78743688010428	35.95220710454809	17.592300066862627
17	20.183283142588493	25.583096887773593	29.00397198523469	25.229647984403226
18	21.58919932191841	28.064518307677645	31.954857596112213	18.391424774291732
19	24.66262889441033	28.91429652036684	27.057601665337167	19.365472919885665
20	26.94886547404552	27.923513957479575	27.136921302462962	17.990699266011948
21	23.915283325691533	28.307955301846118	30.169602944056766	17.607158428405587
22	22.915495695202477	24.565674333231023	29.502027265843857	23.016802705722643
23	19.651008605092514	29.789664133491122	29.2375784472712	21.32174881414516
24	19.61588884144552	28.31200758226692	33.99307960554802	18.079023970739534
25	19.63209796312875	29.4841672151003	31.395267686890797	19.488467134880153
26	18.277435626899038	34.257828593040735	27.568489167278887	19.896246612781344
27	18.627432587688723	32.35213116181131	30.498963291592347	18.521472958907626
28	17.097171433224048	29.930818568149224	34.61833146603251	18.353678532594216
29	18.410860711865602	27.870834312009084	33.733433489696324	19.984871486428986
30	19.927239053777512	29.679277013139142	31.428286268097374	18.965197664985972
31	24.32576432387326	29.216716707327052	26.92642784727105	19.53109112152864
32	24.85826398805928	29.192177898112163	27.38193418346174	18.567623930366814
33	23.30879202271078	29.120362483987865	27.19477886180448	20.376066631496876
34	19.155804929223923	29.023332880578543	29.72632849135851	22.094533698839022
35	19.906452356063376	28.060466027256837	30.2340642196396	21.799017397040185
36	24.33139249112438	28.61757954288776	27.72120010535929	19.32982786062857
37	19.66639226224558	32.34350130535959	28.966150701307157	19.023955731087668
38	19.995302356401066	32.49433618768962	26.458464500897882	21.051896955011433
39	19.924837702417037	30.201570934043133	28.767363833997585	21.10622752954225
40	22.491357011158026	27.202508211496017	28.807961680435668	21.498173096910286
41	19.332079127529017	26.68689304906336	29.42053140404763	24.560496419359996
42	21.152603627691484	27.94947856906474	27.47303545069988	23.424882352543893
43	20.694620897910298	28.761435497826405	27.965162395137867	22.578781209125435
44	20.29749741667123	30.12225129691734	29.00134550718417	20.57890577922726
45	19.237375833250162	32.540562201378826	26.57132801484035	21.65073395053066
46	21.41652715065403	31.353168995852414	27.49749921768475	19.7328046358088
47	20.31963487452564	29.824558770448068	28.177381821620116	21.678424533406172
48	20.401731074161987	29.254613033484596	30.092159362681347	20.25149652967207
49	20.763809833984073	27.968013999878433	29.38458617587047	21.883589990267023
50	19.879136984337936	29.8759626980083	29.882416329789592	20.36248398786417
51	18.637788415430784	31.583323515308237	27.999231567564646	21.77965650169633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	586.0
1	1226.0
2	1866.0
3	5295.5
4	8725.0
5	6312.5
6	3900.0
7	3775.0
8	3650.0
9	3907.5
10	4165.0
11	4176.5
12	4188.0
13	4237.5
14	4287.0
15	4100.0
16	3913.0
17	3762.0
18	3611.0
19	4099.5
20	4588.0
21	4995.0
22	5402.0
23	6500.0
24	7598.0
25	8839.5
26	13841.5
27	17602.0
28	20821.5
29	24041.0
30	29209.5
31	34378.0
32	38130.5
33	41883.0
34	46764.0
35	51645.0
36	57121.0
37	62597.0
38	69780.5
39	76964.0
40	87179.5
41	97395.0
42	108008.0
43	118621.0
44	120857.5
45	123094.0
46	120407.0
47	117720.0
48	119154.5
49	120589.0
50	109667.5
51	98746.0
52	87787.0
53	76828.0
54	67167.0
55	57506.0
56	51413.5
57	45321.0
58	40700.0
59	36079.0
60	30602.5
61	25126.0
62	20052.5
63	14979.0
64	12023.5
65	9068.0
66	7060.0
67	5052.0
68	4154.0
69	3256.0
70	3119.0
71	2982.0
72	2481.0
73	1980.0
74	1690.0
75	969.5
76	539.0
77	428.5
78	318.0
79	229.0
80	140.0
81	108.5
82	77.0
83	56.5
84	36.0
85	35.0
86	34.0
87	24.5
88	15.0
89	11.0
90	7.0
91	5.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1332583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.18480843139405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.37232305587244	17.608597817492512
2	8.226129940782581	3.6499023373603294
3	2.629719359812404	1.7501946067729917
4	1.2863889030774343	1.1415316553217603
5	0.7589188705510569	0.841823487907257
6	0.5718611452105217	0.7611977973511824
7	0.42985322516094787	0.6675348017668765
8	0.34234496101663564	0.60758859020857
9	0.2986408042502666	0.5962760128880653
>10	3.7090046416695603	19.899271215636492
>50	1.4712716577506466	23.5731793452122
>100	0.8991292805226859	27.71361454055451
>500	0.003055952992713	0.43042756782284003
>1k	0.0010186509975709998	0.3422931165885518
>5k	3.3955033252366663E-4	0.4165671071159093
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5530	0.4149835319826233	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2032	0.15248581139035994	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1393	0.10453382641081269	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04742668936944265	0.0
2	0.0	0.0	0.0	0.1786755496655743	0.0
3	0.0	0.0	0.0	0.26369839627250236	0.0
4	0.0	0.0	0.0	0.39006951161766285	0.0
5	0.0	0.0	0.0	0.6931650786480091	0.0
6	0.0	0.0	0.0	0.93967880424709	0.0
7	0.0	0.0	0.0	1.1037961612897658	0.0
8	0.0	0.0	0.0	1.4777315934542163	0.0
9	0.0	0.0	0.0	1.5966735280278976	0.0
10	0.0	0.0	0.0	1.8574452773298173	0.0
11	0.0	0.0	0.0	2.2633486994806327	0.0
12	0.0	0.0	0.0	2.5811525435939076	0.0
13	0.0	0.0	0.0	2.7096248413794863	0.0
14	0.0	0.0	0.0	2.758027079739123	0.0
15	0.0	0.0	0.0	2.8490533047472466	0.0
16	0.0	0.0	0.0	3.0733545302619047	0.0
17	0.0	0.0	0.0	3.3579897087085757	0.0
18	0.0	0.0	0.0	3.689226111994525	0.0
19	0.0	0.0	0.0	3.881259178602759	0.0
20	0.0	0.0	0.0	4.060234897188392	0.0
21	0.0	0.0	0.0	4.298268850795785	0.0
22	0.0	0.0	0.0	4.568946174459677	0.0
23	0.0	0.0	0.0	4.856507999876931	0.0
24	0.0	0.0	0.0	5.074805846990394	0.0
25	0.0	0.0	0.0	5.264212435548105	0.0
26	0.0	0.0	0.0	5.448966405844889	0.0
27	0.0	0.0	0.0	5.638898290012705	0.0
28	0.0	0.0	0.0	5.8508925898049124	0.0
29	0.0	0.0	0.0	6.081197193720766	0.0
30	0.0	0.0	0.0	6.346621561283612	0.0
31	0.0	0.0	0.0	6.5938857091828424	0.0
32	0.0	0.0	0.0	6.809331951555738	0.0
33	0.0	0.0	0.0	7.036034528430874	0.0
34	0.0	0.0	0.0	7.2695659482373705	0.0
35	0.0	0.0	0.0	7.525384910358304	0.0
36	0.0	0.0	0.0	7.760792385915174	0.0
37	0.0	0.0	0.0	8.003403915553477	0.0
38	0.0	0.0	0.0	8.274681577057489	0.0
39	0.0	0.0	0.0	8.59803854619187	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	35	1.21212E-7	45.000004	27
TACGAAC	35	1.21212E-7	45.000004	41
AGCGCGC	35	1.21212E-7	45.000004	36
CCGCGTG	35	1.21212E-7	45.000004	33
CTTCGGT	40	6.8157533E-9	45.0	34
AACCGAT	20	7.033692E-4	45.0	26
CGCGACT	25	3.8911414E-5	45.0	43
AATATCG	25	3.8911414E-5	45.0	1
TAGCACG	20	7.033692E-4	45.0	1
TCGCTAG	25	3.8911414E-5	45.0	1
TTGCGAG	90	0.0	45.0	1
CCGGTCA	25	3.8911414E-5	45.0	31
GCGGACA	20	7.033692E-4	45.0	14
CGCGTCC	20	7.033692E-4	45.0	17
TATCGCG	25	3.8911414E-5	45.0	1
ATACCGG	40	6.8157533E-9	45.0	2
ATCGCAA	25	3.8911414E-5	45.0	25
AATCCGG	60	0.0	44.999996	2
CGTGCGA	30	2.1657634E-6	44.999996	39
CTATCCG	30	2.1657634E-6	44.999996	1
>>END_MODULE