FastQCFastQC Report
Thu 26 May 2016
SRR1548091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548091_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences968280
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35450.3661131077787417No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16900.1745362911554509No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG453.8380676E-1045.0000041
AGCGCAT453.8380676E-1045.00000410
CGCGTGA453.8380676E-1045.00000412
CGATAGT453.8380676E-1045.00000410
ACGGTCC453.8380676E-1045.00000433
ACGCGTG453.8380676E-1045.00000411
CTAGATA1000.045.019
TTTAGCG207.032737E-445.01
TTAGGTA253.8903483E-545.018
CTATGCG406.8139343E-945.01
AGCGTAT406.8139343E-945.029
AACCGAT207.032737E-445.035
TCGCATA253.8903483E-545.014
AGTCCGT207.032737E-445.033
TCGCACA207.032737E-445.017
GCGACGC207.032737E-445.042
TCCGCAA406.8139343E-945.032
CTCGTCG207.032737E-445.044
GTACAGT207.032737E-445.016
AGTCAAT351.2116652E-745.030