##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548091_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	968280
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.625327384640805	31.0	31.0	33.0	30.0	34.0
2	32.03146197380923	31.0	31.0	34.0	30.0	34.0
3	32.0952121287231	33.0	31.0	34.0	30.0	34.0
4	35.78638410377164	37.0	35.0	37.0	35.0	37.0
5	35.700958400462675	37.0	35.0	37.0	33.0	37.0
6	35.7669795926798	37.0	35.0	37.0	34.0	37.0
7	36.11767877060355	37.0	35.0	37.0	35.0	37.0
8	36.145981534266944	37.0	35.0	37.0	35.0	37.0
9	38.00990106167637	39.0	39.0	39.0	35.0	39.0
10	37.47356136654687	39.0	37.0	39.0	35.0	39.0
11	37.07211653653902	39.0	37.0	39.0	33.0	39.0
12	35.84150865452142	37.0	35.0	39.0	32.0	39.0
13	35.24624385508324	37.0	35.0	39.0	30.0	39.0
14	36.21599434048002	38.0	35.0	41.0	31.0	41.0
15	36.61766121369852	38.0	35.0	41.0	32.0	41.0
16	36.85814537117363	38.0	35.0	41.0	33.0	41.0
17	36.81329677366051	38.0	35.0	41.0	32.0	41.0
18	36.782709546825295	37.0	35.0	40.0	32.0	41.0
19	36.70368178626017	37.0	35.0	40.0	32.0	41.0
20	36.53261969678192	37.0	35.0	40.0	32.0	41.0
21	36.36670281323584	37.0	35.0	40.0	32.0	41.0
22	36.25538583880696	36.0	35.0	40.0	31.0	41.0
23	36.27545338125336	36.0	35.0	40.0	32.0	41.0
24	36.248365142314206	36.0	35.0	40.0	32.0	41.0
25	36.213879249803774	36.0	35.0	40.0	32.0	41.0
26	36.10259222538935	36.0	35.0	40.0	31.0	41.0
27	36.02232412112199	36.0	35.0	40.0	31.0	41.0
28	36.071659024249186	36.0	35.0	40.0	31.0	41.0
29	36.10908311645392	36.0	35.0	40.0	31.0	41.0
30	36.08893708431445	36.0	35.0	40.0	31.0	41.0
31	35.89425372826042	36.0	35.0	40.0	31.0	41.0
32	35.72388255463296	36.0	34.0	40.0	30.0	41.0
33	35.619727764696165	36.0	35.0	40.0	30.0	41.0
34	35.565068988309164	36.0	35.0	40.0	30.0	41.0
35	35.479492502168796	36.0	35.0	40.0	30.0	41.0
36	35.2770376337423	36.0	34.0	40.0	29.0	41.0
37	35.18013694385922	35.0	34.0	40.0	29.0	41.0
38	35.13566427066551	35.0	34.0	40.0	29.0	41.0
39	35.159889701326065	36.0	34.0	40.0	29.0	41.0
40	35.001168050563884	36.0	34.0	40.0	28.0	41.0
41	34.976494402445574	36.0	34.0	40.0	29.0	41.0
42	34.90334923782377	35.0	34.0	40.0	28.0	41.0
43	34.79470917503202	35.0	34.0	40.0	27.0	41.0
44	34.64970256537365	35.0	34.0	40.0	27.0	41.0
45	34.595020035526915	35.0	34.0	40.0	27.0	41.0
46	34.53290370553972	35.0	34.0	40.0	27.0	41.0
47	34.470016937249554	35.0	34.0	40.0	27.0	41.0
48	34.42700665096873	35.0	34.0	40.0	27.0	41.0
49	34.36978663196596	35.0	34.0	40.0	26.0	41.0
50	34.18538542570331	35.0	34.0	39.0	26.0	41.0
51	33.78025158012145	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	7.0
15	16.0
16	56.0
17	138.0
18	314.0
19	648.0
20	1089.0
21	1948.0
22	3078.0
23	4560.0
24	6685.0
25	9713.0
26	12713.0
27	15079.0
28	16876.0
29	19299.0
30	23191.0
31	28940.0
32	37227.0
33	50524.0
34	102846.0
35	190083.0
36	53792.0
37	66184.0
38	107596.0
39	215634.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.212562481926714	24.130933201140166	31.185194365266245	13.471309951666873
2	29.0203246994671	25.725719833106126	32.25451315735118	12.9994423100756
3	29.409468335605403	26.06384516875284	31.074379311769324	13.452307183872433
4	25.132296443177594	27.983847647374727	31.78336844714339	15.100487462304294
5	24.19393150741521	32.0896848019168	29.645660346180858	14.070723344487131
6	21.212562481926717	41.068699136613375	26.448341388854463	11.270396992605445
7	89.68490519271285	3.6261205436444004	5.375201388028256	1.3137728756144917
8	90.81691246333705	2.5935679762052297	4.843227165695873	1.7462923947618458
9	87.07667203701409	4.7163010699384476	5.933924071549552	2.273102821497914
10	56.29600941876317	25.72117569298137	10.012083281695377	7.9707316065600855
11	51.415912752509605	17.742595117114885	19.499938034452843	11.341554095922666
12	48.17263601437601	19.45139835584748	19.95590118560747	12.420064444169041
13	20.23577890692775	45.085099351427274	21.724811005081175	12.954310736563803
14	13.12450943941835	46.99869872350973	25.819907464782915	14.056884372289009
15	11.811562771099268	25.005990002891725	51.94396249018879	11.238484735820217
16	14.508406659230802	18.43733217664312	51.24664353286239	15.807617631263685
17	15.238154252902053	18.502499277068615	27.296339901681332	38.963006568348
18	21.10009501383897	24.12773164787045	36.068079481141815	18.70409385714876
19	30.79130003717933	25.174949394803157	25.03707605238154	18.996674515635974
20	34.52740942702524	24.108625604164086	23.912607923327965	17.451357045482712
21	22.368013384558186	29.043561779650513	28.164683769157683	20.423741066633617
22	21.714070310240842	23.989238649977278	25.985871855248483	28.310819184533397
23	20.05473623332094	31.95532284050068	24.33056553889371	23.65937538728467
24	19.818647498657413	23.109740984012888	40.74482587681249	16.326785640517205
25	16.013343247820877	25.000722931383486	38.88059239063081	20.105341430164827
26	15.908311645391828	38.95856570413517	26.171252117156197	18.96187053331681
27	17.373383731978354	37.05519064733341	29.96850084686248	15.602924773825752
28	13.96238691287644	29.407815921014585	41.68236460527947	14.947432560829514
29	13.386933531623086	25.52278266617094	40.96593960424671	20.124344197959267
30	17.3134837030611	32.72193993472962	32.81055066716239	17.154025695046887
31	30.665096872805385	26.95480646094105	24.617362746313052	17.762733919940512
32	31.82829346883133	27.67598215392242	25.592080803073493	14.90364357417276
33	28.40500681621019	28.934708968480194	24.03943074317346	18.620853472136158
34	18.024331804849837	29.797062833064814	27.438963936051557	24.739641426033792
35	17.985809889701326	25.886933531623086	32.812099805841285	23.315156772834303
36	31.64931631346305	23.4576775312926	27.800326351881687	17.092679803362664
37	18.8899904986161	32.69859957863428	30.43045400090883	17.980955921840792
38	18.7568678481431	33.88575618622712	23.667224356591067	23.690151609038708
39	18.555479819886813	32.84143016482835	28.992853306894702	19.610236708390136
40	25.137666790597763	25.825484364026934	25.386665014252074	23.65018383112323
41	16.340004957243774	23.444974594125668	29.213863758416984	31.001156690213577
42	22.799500144586275	24.373115214607346	25.3569215516173	27.470463089189078
43	22.088858594621392	25.651154624695337	25.722931383484116	26.537055397199154
44	18.775767340025613	31.59396042467055	30.10059073821622	19.52968149708762
45	17.01842442268765	40.82600074358657	21.919795926798034	20.23577890692775
46	22.691473540711364	34.63078861486347	24.25228239765357	18.425455446771593
47	20.96418391374396	28.3920973272194	26.901619366299006	23.742099392737636
48	23.719895071673484	23.512310488701615	31.80371380179287	20.964080637832033
49	19.679741397116537	22.51332259263849	34.08817697360268	23.718759036642293
50	19.077333002850416	34.913661337629605	26.611723881521876	19.3972817779981
51	17.06871979179576	36.34589168422357	24.219234105837153	22.366154418143513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	573.0
1	1015.0
2	1457.0
3	3771.0
4	6085.0
5	4669.5
6	3254.0
7	3318.0
8	3382.0
9	3612.5
10	3843.0
11	3682.0
12	3521.0
13	3274.0
14	3027.0
15	2905.0
16	2783.0
17	2682.0
18	2581.0
19	3087.0
20	3593.0
21	3538.0
22	3483.0
23	4612.0
24	5741.0
25	6088.5
26	8773.5
27	11111.0
28	11232.0
29	11353.0
30	13636.0
31	15919.0
32	17747.5
33	19576.0
34	20222.0
35	20868.0
36	23963.0
37	27058.0
38	33840.5
39	40623.0
40	58375.0
41	76127.0
42	99589.5
43	123052.0
44	121030.0
45	119008.0
46	109501.0
47	99994.0
48	90203.5
49	80413.0
50	72447.5
51	64482.0
52	58725.5
53	52969.0
54	45717.0
55	38465.0
56	35351.5
57	32238.0
58	29213.0
59	26188.0
60	24492.0
61	22796.0
62	18578.0
63	14360.0
64	12044.5
65	9729.0
66	7640.5
67	5552.0
68	4136.0
69	2720.0
70	2222.0
71	1724.0
72	1340.0
73	956.0
74	886.5
75	485.5
76	154.0
77	127.0
78	100.0
79	72.0
80	44.0
81	39.5
82	35.0
83	35.5
84	36.0
85	27.5
86	19.0
87	21.5
88	24.0
89	17.5
90	11.0
91	5.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	968280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.196690584062445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74321827776012	14.057128320681183
2	8.260397110584124	2.841029864243972
3	2.4454641955407506	1.2616167331535237
4	1.100104522785209	0.7567262835385964
5	0.5480280530254735	0.47121344296326173
6	0.3666709790017345	0.37833164232288513
7	0.2601091402249755	0.313111148177479
8	0.18888055038098875	0.2598496305799425
9	0.1549155771318739	0.23976317219294657
>10	1.7892596007858257	7.778190377135015
>50	1.1550453960816254	14.853826746909196
>100	1.9824866756520911	55.811598841288045
>500	0.004215494146283304	0.43547309487316666
>1k	0.001204426898938087	0.5421407019407913
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3545	0.3661131077787417	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1690	0.1745362911554509	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.042239847977857646	0.0
2	0.0	0.0	0.0	0.1571859379518321	0.0
3	0.0	0.0	0.0	0.22400545296814972	0.0
4	0.0	0.0	0.0	0.3632213822448052	0.0
5	0.0	0.0	0.0	0.6059197752716157	0.0
6	0.0	0.0	0.0	0.8305448837113232	0.0
7	0.0	0.0	0.0	0.9606725327384641	0.0
8	0.0	0.0	0.0	1.2576940554385094	0.0
9	0.0	0.0	0.0	1.3420704754822985	0.0
10	0.0	0.0	0.0	1.5418060891477672	0.0
11	0.0	0.0	0.0	1.7978270748130707	0.0
12	0.0	0.0	0.0	2.0154294212417896	0.0
13	0.0	0.0	0.0	2.1041434295864834	0.0
14	0.0	0.0	0.0	2.136055686371711	0.0
15	0.0	0.0	0.0	2.203804684595365	0.0
16	0.0	0.0	0.0	2.353038377328872	0.0
17	0.0	0.0	0.0	2.5247862188623125	0.0
18	0.0	0.0	0.0	2.772235303837733	0.0
19	0.0	0.0	0.0	2.8824307018630972	0.0
20	0.0	0.0	0.0	2.998409550956335	0.0
21	0.0	0.0	0.0	3.167162391043913	0.0
22	0.0	0.0	0.0	3.33901350848928	0.0
23	0.0	0.0	0.0	3.5266658404593714	0.0
24	0.0	0.0	0.0	3.6531788325690915	0.0
25	0.0	0.0	0.0	3.758623538645846	0.0
26	0.0	0.0	0.0	3.8689222125831373	0.0
27	0.0	0.0	0.0	3.9674474325608293	0.0
28	0.0	0.0	0.0	4.0776428305861945	0.0
29	0.0	0.0	0.0	4.20260668401702	0.0
30	0.0	0.0	0.0	4.370946420456892	0.0
31	0.0	0.0	0.0	4.538150121865576	0.0
32	0.0	0.0	0.0	4.673648118312885	0.0
33	0.0	0.0	0.0	4.805118354195067	0.0
34	0.0	0.0	0.0	4.956107737431322	0.0
35	0.0	0.0	0.0	5.161936629900442	0.0
36	0.0	0.0	0.0	5.334407402817367	0.0
37	0.0	0.0	0.0	5.49717024001322	0.0
38	0.0	0.0	0.0	5.639484446647664	0.0
39	0.0	0.0	0.0	5.795534349568307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	45	3.8380676E-10	45.000004	1
AGCGCAT	45	3.8380676E-10	45.000004	10
CGCGTGA	45	3.8380676E-10	45.000004	12
CGATAGT	45	3.8380676E-10	45.000004	10
ACGGTCC	45	3.8380676E-10	45.000004	33
ACGCGTG	45	3.8380676E-10	45.000004	11
CTAGATA	100	0.0	45.0	19
TTTAGCG	20	7.032737E-4	45.0	1
TTAGGTA	25	3.8903483E-5	45.0	18
CTATGCG	40	6.8139343E-9	45.0	1
AGCGTAT	40	6.8139343E-9	45.0	29
AACCGAT	20	7.032737E-4	45.0	35
TCGCATA	25	3.8903483E-5	45.0	14
AGTCCGT	20	7.032737E-4	45.0	33
TCGCACA	20	7.032737E-4	45.0	17
GCGACGC	20	7.032737E-4	45.0	42
TCCGCAA	40	6.8139343E-9	45.0	32
CTCGTCG	20	7.032737E-4	45.0	44
GTACAGT	20	7.032737E-4	45.0	16
AGTCAAT	35	1.2116652E-7	45.0	30
>>END_MODULE