FastQCFastQC Report
Thu 26 May 2016
SRR1548090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548090_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences455865
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32480.7124916367784322No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT5820.12766937580204668No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC253.8870887E-545.00000413
CGAACGG253.8870887E-545.0000042
TCTAGCG253.8870887E-545.0000041
CGTTCAC253.8870887E-545.00000421
ACGGGCT502.1827873E-1145.0000045
TACGCGG253.8870887E-545.0000042
GTTCGAT351.2097735E-745.00000439
CTCGAAG253.8870887E-545.0000041
TATCGGA253.8870887E-545.00000410
ACTGCAC453.8380676E-1045.033
GGTAATC207.028803E-445.09
CGTATGG207.028803E-445.02
TTTCGTA207.028803E-445.027
CACGACG1050.045.026
TAGCCGG207.028803E-445.02
CTAACGG406.7993824E-945.02
CGTAGTA207.028803E-445.030
TACGATG406.7993824E-945.01
CTACGGT207.028803E-445.015
TTAAGCG207.028803E-445.01