##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548090_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	455865
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00886666008577	31.0	31.0	33.0	31.0	34.0
2	32.47843111447468	33.0	31.0	34.0	31.0	34.0
3	32.53287705790091	34.0	31.0	34.0	31.0	34.0
4	36.1991466771961	37.0	35.0	37.0	35.0	37.0
5	36.17166485692036	37.0	35.0	37.0	35.0	37.0
6	36.24335274697553	37.0	36.0	37.0	35.0	37.0
7	36.37050223202044	37.0	36.0	37.0	35.0	37.0
8	36.389534182268875	37.0	37.0	37.0	35.0	37.0
9	38.27423908393932	39.0	39.0	39.0	37.0	39.0
10	37.96444780801334	39.0	38.0	39.0	35.0	39.0
11	37.68626238030996	39.0	37.0	39.0	35.0	39.0
12	37.09499961611442	39.0	35.0	39.0	35.0	39.0
13	36.86615774406897	39.0	35.0	39.0	33.0	39.0
14	37.942689173329825	40.0	35.0	41.0	33.0	41.0
15	38.14044289427791	40.0	35.0	41.0	33.0	41.0
16	38.2714356223882	40.0	35.0	41.0	34.0	41.0
17	38.24180184923168	40.0	35.0	41.0	34.0	41.0
18	38.14044289427791	40.0	36.0	41.0	34.0	41.0
19	38.08105908547487	40.0	36.0	41.0	34.0	41.0
20	37.92052032948351	40.0	35.0	41.0	34.0	41.0
21	37.82470248867538	40.0	35.0	41.0	34.0	41.0
22	37.77114277253134	40.0	35.0	41.0	34.0	41.0
23	37.695600671251356	39.0	35.0	41.0	34.0	41.0
24	37.70518903622783	39.0	35.0	41.0	34.0	41.0
25	37.6954295679642	39.0	35.0	41.0	34.0	41.0
26	37.6065808956599	39.0	35.0	41.0	34.0	41.0
27	37.5741853399581	39.0	35.0	41.0	34.0	41.0
28	37.55439219944501	39.0	35.0	41.0	34.0	41.0
29	37.50430280894563	39.0	35.0	41.0	34.0	41.0
30	37.42208548583462	39.0	35.0	41.0	34.0	41.0
31	37.30609500619701	39.0	35.0	41.0	33.0	41.0
32	37.18485735908657	39.0	35.0	41.0	33.0	41.0
33	37.07671788797122	39.0	35.0	41.0	33.0	41.0
34	36.95314841016529	39.0	35.0	41.0	33.0	41.0
35	36.82486920469876	39.0	35.0	41.0	32.0	41.0
36	36.68363879657355	39.0	35.0	41.0	32.0	41.0
37	36.60986476259419	39.0	35.0	41.0	32.0	41.0
38	36.48831781338774	39.0	35.0	41.0	31.0	41.0
39	36.41706206881423	39.0	35.0	41.0	31.0	41.0
40	36.312449957772586	39.0	35.0	41.0	31.0	41.0
41	36.222517631316286	39.0	35.0	41.0	31.0	41.0
42	36.188108321542565	38.0	35.0	41.0	31.0	41.0
43	36.11281190703388	38.0	35.0	41.0	31.0	41.0
44	35.96862887038926	38.0	35.0	41.0	30.0	41.0
45	35.92030974082239	38.0	35.0	40.0	30.0	41.0
46	35.8769679620063	38.0	35.0	40.0	30.0	41.0
47	35.79398286773497	38.0	35.0	40.0	30.0	41.0
48	35.69598016956775	38.0	35.0	40.0	30.0	41.0
49	35.624733199521785	38.0	35.0	40.0	29.0	41.0
50	35.501598060829416	37.0	35.0	40.0	29.0	41.0
51	35.092297061630084	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	5.0
13	3.0
14	8.0
15	14.0
16	26.0
17	31.0
18	88.0
19	141.0
20	285.0
21	504.0
22	771.0
23	1255.0
24	2018.0
25	3055.0
26	4387.0
27	5092.0
28	5109.0
29	5301.0
30	5974.0
31	7501.0
32	10266.0
33	15279.0
34	33601.0
35	62272.0
36	26998.0
37	38964.0
38	70880.0
39	156007.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.51488927643052	23.401664966601953	30.37522073420859	13.70822502275893
2	31.285139240784005	25.329209305386463	30.061970100797385	13.323681353032146
3	29.658561196845557	25.29937591172825	31.71882026477137	13.323242626654821
4	27.19577067772257	27.153214219121892	30.826012086911696	14.825003016243842
5	23.616640891492	30.949732925317804	30.959165542430323	14.474460640759872
6	22.323714257510446	40.486108826077896	26.713610389040614	10.476566527371043
7	88.11490243822185	3.8030996018558123	6.484375856887456	1.5976221030348896
8	88.9612056200849	2.99979160497077	6.400798482006735	1.638204292937602
9	83.86583747381351	5.672512695644544	7.523279918396894	2.938369912145043
10	45.958781656850164	30.06021519528808	13.74047141149244	10.24053173636932
11	37.18359602075176	22.587388810283745	24.907812619964243	15.321202549000251
12	34.16954580851787	22.55601987430489	27.035635549998354	16.23879876717888
13	23.269827690215305	32.904917025873885	27.214635911947617	16.61061937196319
14	16.81396904785408	35.35981047020499	30.192052471674728	17.634168010266198
15	15.892424292279514	26.57277922191877	41.48333388174131	16.05146260406041
16	20.432145481666723	23.51990172529148	38.62810261809966	17.41985017494214
17	20.824147499808056	23.202263828107007	28.462812455441856	27.510776216643084
18	23.000230331348096	26.180119114211443	32.71933576826473	18.10031478617573
19	27.31422679960076	27.204106478891777	26.103561361367948	19.378105360139514
20	28.554944994680444	26.965878056003422	26.9325348513266	17.546642097989537
21	24.210018316826254	27.7755475853597	30.077764250381144	17.936669847432903
22	24.230199730183276	22.757614644686473	29.86300768867976	23.149177936450485
23	20.26038410494335	28.267359854342843	29.775701139591764	21.69655490112204
24	20.147412062781743	25.707830169019335	35.34884231077183	18.79591545742709
25	20.64799885931142	27.002950434887524	32.705296524190274	19.643754181610785
26	18.19705395237625	33.9631250479857	27.25806982330295	20.5817511763351
27	18.235661873580995	31.58522808287541	32.03360644050322	18.145503603040375
28	16.40836651201562	28.323736193829312	36.83327300845645	18.434624285698618
29	17.573843133383786	27.663891722330074	35.57763811654766	19.184627027738475
30	20.18558125760916	29.661851644675508	31.90198852730523	18.2505785704101
31	26.83952485933336	27.334408212957783	27.054281421034737	18.771785506674124
32	27.013260504754697	27.96463865398747	27.643271582595723	17.378829258662105
33	25.7170434229432	28.19957662904588	25.86028758514034	20.223092362870588
34	18.99685213824268	28.625360578241366	29.919822754543564	22.457964528972393
35	20.281881697432354	26.903578910423043	31.22942099086352	21.585118401281083
36	26.59976089412436	26.611167779934846	28.81774209469909	17.971329231241707
37	19.894266943064284	31.394601471926997	29.613153016792253	19.09797856821647
38	20.631107893784346	31.91427286587038	26.20227479626644	21.252344444078837
39	19.945378566022836	30.426990446733132	28.748423327081486	20.87920766016255
40	23.71667050552247	26.562030425674266	27.8975135182565	21.823785550546763
41	19.082184418632707	25.072773737839054	29.618417733320175	26.226624110208064
42	21.254538075965474	26.37118445153719	27.65577528434953	24.718502188147806
43	21.484650060873285	27.36665460169129	27.548067958715848	23.600627378719576
44	20.089719544163295	29.082294100227042	29.948559332258455	20.87942702335121
45	19.46804426749147	33.40572318559222	25.59792921149902	21.528303335417284
46	22.535619097759206	30.170116152808397	26.70330031917344	20.59096443025896
47	20.015136060017767	28.84823357792329	28.868195628091648	22.268434733967293
48	21.328902196922332	27.023131848244546	31.368716615664727	20.279249339168395
49	20.84213528127845	26.337183157294376	31.009180349445558	21.811501211981618
50	19.717460213001655	30.778190911783092	28.797999407719388	20.706349467495862
51	18.51162076491944	32.175973149945705	27.485330086758143	21.827075998376714
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	284.0
1	581.5
2	879.0
3	2960.5
4	5042.0
5	3535.0
6	2028.0
7	1898.0
8	1768.0
9	1853.5
10	1939.0
11	1936.5
12	1934.0
13	1877.0
14	1820.0
15	1823.5
16	1827.0
17	1875.5
18	1924.0
19	1735.0
20	1546.0
21	1691.5
22	1837.0
23	2277.0
24	2717.0
25	3085.5
26	4209.0
27	4964.0
28	5757.0
29	6550.0
30	8471.0
31	10392.0
32	11437.5
33	12483.0
34	13334.5
35	14186.0
36	15804.0
37	17422.0
38	19638.5
39	21855.0
40	27369.0
41	32883.0
42	35992.5
43	39102.0
44	40704.0
45	42306.0
46	39652.5
47	36999.0
48	36990.5
49	36982.0
50	34790.0
51	32598.0
52	29170.5
53	25743.0
54	24082.5
55	22422.0
56	20305.5
57	18189.0
58	16632.5
59	15076.0
60	13509.0
61	11942.0
62	10336.5
63	8731.0
64	7347.0
65	5963.0
66	4940.0
67	3917.0
68	3209.0
69	2501.0
70	2116.5
71	1732.0
72	1329.5
73	927.0
74	719.0
75	358.0
76	205.0
77	168.5
78	132.0
79	98.0
80	64.0
81	46.0
82	28.0
83	30.5
84	33.0
85	22.5
86	12.0
87	9.0
88	6.0
89	5.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	455865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.170305277444662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38009466508706	16.711997105676577
2	6.5220752810128175	2.891928000450597
3	2.4449120482968043	1.6261333946172807
4	1.4327293244703894	1.270561860138224
5	1.035795683502294	1.1481953254152653
6	0.8268365265343871	1.0998730924685602
7	0.6898635903308377	1.0706140478200086
8	0.6098794051232923	1.0816970075207788
9	0.5248962092889176	1.0473398279017907
>10	9.042212031979	49.341564662387846
>50	1.4247182823496127	19.698411814938204
>100	0.06398734741780716	2.162729037205204
>500	9.99802303403237E-4	0.1290056676902151
>1k	9.99802303403237E-4	0.7199491557694478
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3248	0.7124916367784322	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	582	0.12766937580204668	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0315882991675167	0.0
2	0.0	0.0	0.0	0.12174656970813728	0.0
3	0.0	0.0	0.0	0.1849231680431707	0.0
4	0.0	0.0	0.0	0.2803461551117107	0.0
5	0.0	0.0	0.0	0.49181226898314195	0.0
6	0.0	0.0	0.0	0.6433922323494894	0.0
7	0.0	0.0	0.0	0.7460542046439187	0.0
8	0.0	0.0	0.0	0.9719982889671285	0.0
9	0.0	0.0	0.0	1.0492141313766137	0.0
10	0.0	0.0	0.0	1.215491428383403	0.0
11	0.0	0.0	0.0	1.482017702609325	0.0
12	0.0	0.0	0.0	1.6783477564629878	0.0
13	0.0	0.0	0.0	1.764557489607669	0.0
14	0.0	0.0	0.0	1.804042863567065	0.0
15	0.0	0.0	0.0	1.8698518201660579	0.0
16	0.0	0.0	0.0	2.0030052756846874	0.0
17	0.0	0.0	0.0	2.149759248900442	0.0
18	0.0	0.0	0.0	2.3261272525857435	0.0
19	0.0	0.0	0.0	2.4292279512574995	0.0
20	0.0	0.0	0.0	2.5555811479275663	0.0
21	0.0	0.0	0.0	2.711767738255843	0.0
22	0.0	0.0	0.0	2.87124477641407	0.0
23	0.0	0.0	0.0	3.054413038947934	0.0
24	0.0	0.0	0.0	3.1917343950511663	0.0
25	0.0	0.0	0.0	3.3042677108354446	0.0
26	0.0	0.0	0.0	3.4216270167703158	0.0
27	0.0	0.0	0.0	3.5486383030063724	0.0
28	0.0	0.0	0.0	3.681791758525002	0.0
29	0.0	0.0	0.0	3.8316168163820428	0.0
30	0.0	0.0	0.0	3.9989909293321486	0.0
31	0.0	0.0	0.0	4.161539052131662	0.0
32	0.0	0.0	0.0	4.301492766498854	0.0
33	0.0	0.0	0.0	4.451975913921885	0.0
34	0.0	0.0	0.0	4.612111041646101	0.0
35	0.0	0.0	0.0	4.7990084783872415	0.0
36	0.0	0.0	0.0	4.966163228148685	0.0
37	0.0	0.0	0.0	5.13814396806072	0.0
38	0.0	0.0	0.0	5.338641922498986	0.0
39	0.0	0.0	0.0	5.634343500817128	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTC	25	3.8870887E-5	45.000004	13
CGAACGG	25	3.8870887E-5	45.000004	2
TCTAGCG	25	3.8870887E-5	45.000004	1
CGTTCAC	25	3.8870887E-5	45.000004	21
ACGGGCT	50	2.1827873E-11	45.000004	5
TACGCGG	25	3.8870887E-5	45.000004	2
GTTCGAT	35	1.2097735E-7	45.000004	39
CTCGAAG	25	3.8870887E-5	45.000004	1
TATCGGA	25	3.8870887E-5	45.000004	10
ACTGCAC	45	3.8380676E-10	45.0	33
GGTAATC	20	7.028803E-4	45.0	9
CGTATGG	20	7.028803E-4	45.0	2
TTTCGTA	20	7.028803E-4	45.0	27
CACGACG	105	0.0	45.0	26
TAGCCGG	20	7.028803E-4	45.0	2
CTAACGG	40	6.7993824E-9	45.0	2
CGTAGTA	20	7.028803E-4	45.0	30
TACGATG	40	6.7993824E-9	45.0	1
CTACGGT	20	7.028803E-4	45.0	15
TTAAGCG	20	7.028803E-4	45.0	1
>>END_MODULE