FastQCFastQC Report
Thu 26 May 2016
SRR1548088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548088_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1179897
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36390.30841675163171023No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG15140.1283162852350671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATG551.8189894E-1245.0000049
TAGTACG551.8189894E-1245.0000041
TACGAAC253.8908685E-545.031
CGTAAAC207.033363E-445.041
ACGTAGC207.033363E-445.016
ATCGATT253.8908685E-545.013
CCGATTA302.1655524E-644.99999612
CGGTCTA4850.043.14433331
TCACGAC5050.042.77227825
TACGTAG1000.042.751
AATACGG900.042.52
CGACGGT5100.042.3529428
GACGGTC5100.042.3529429
CACGACG5050.041.8811926
CGCATCG700.041.78571721
CTCACGA5300.041.60377524
TACGGGA5150.041.5048524
CGACAAT603.6379788E-1241.24999620
TACGCGG603.6379788E-1241.2499962
CACGTAG501.0822987E-940.51
Produced by FastQC (version 0.11.5)