##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548088_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1179897
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.455278723481797	31.0	31.0	33.0	30.0	34.0
2	31.860709027991426	31.0	31.0	34.0	30.0	34.0
3	31.972658630371974	33.0	31.0	34.0	30.0	34.0
4	35.698065170095354	37.0	35.0	37.0	33.0	37.0
5	35.5853146503466	37.0	35.0	37.0	33.0	37.0
6	35.63577244454389	37.0	35.0	37.0	33.0	37.0
7	35.98592165248323	37.0	35.0	37.0	35.0	37.0
8	35.98720566286718	37.0	35.0	37.0	35.0	37.0
9	37.75522778683224	39.0	37.0	39.0	35.0	39.0
10	37.22869199599626	39.0	37.0	39.0	34.0	39.0
11	36.92913957743769	39.0	37.0	39.0	33.0	39.0
12	36.427450870711596	39.0	35.0	39.0	32.0	39.0
13	36.22226770641844	39.0	35.0	39.0	32.0	39.0
14	37.303100185863684	39.0	35.0	41.0	32.0	41.0
15	37.46473293855311	39.0	35.0	41.0	32.0	41.0
16	37.57066337146378	39.0	35.0	41.0	33.0	41.0
17	37.48051228200428	39.0	35.0	41.0	32.0	41.0
18	37.41037310883916	39.0	36.0	41.0	32.0	41.0
19	37.37162904897631	39.0	35.0	41.0	32.0	41.0
20	37.259028542321914	39.0	35.0	41.0	32.0	41.0
21	37.14152845544992	39.0	35.0	41.0	32.0	41.0
22	37.054218291935655	39.0	35.0	41.0	32.0	41.0
23	36.964585044287766	39.0	35.0	41.0	31.0	41.0
24	36.991309410906204	39.0	35.0	41.0	31.0	41.0
25	36.98671409453537	39.0	35.0	41.0	31.0	41.0
26	36.85055814193951	39.0	35.0	41.0	31.0	41.0
27	36.78669239772624	39.0	35.0	41.0	31.0	41.0
28	36.748244126394084	39.0	35.0	41.0	31.0	41.0
29	36.68444194705131	39.0	35.0	41.0	31.0	41.0
30	36.59223389838265	38.0	35.0	41.0	31.0	41.0
31	36.5326634443515	38.0	35.0	41.0	30.0	41.0
32	36.427794121012255	38.0	35.0	41.0	30.0	41.0
33	36.34579713313959	38.0	35.0	41.0	30.0	41.0
34	36.244970535563695	38.0	35.0	41.0	30.0	41.0
35	36.11117411096053	38.0	35.0	41.0	30.0	41.0
36	35.969140526673094	38.0	35.0	41.0	29.0	41.0
37	35.87490772499633	38.0	35.0	40.0	29.0	41.0
38	35.76887304569806	38.0	35.0	40.0	28.0	41.0
39	35.67031868035939	38.0	35.0	40.0	28.0	41.0
40	35.53024374161473	38.0	34.0	40.0	27.0	41.0
41	35.43123086167691	38.0	34.0	40.0	27.0	41.0
42	35.3706993068039	38.0	34.0	40.0	27.0	41.0
43	35.27198052033356	38.0	34.0	40.0	26.0	41.0
44	35.09981040717961	38.0	34.0	40.0	26.0	41.0
45	35.04568873384711	38.0	34.0	40.0	26.0	41.0
46	34.954625700378934	38.0	34.0	40.0	25.0	41.0
47	34.85858680884857	37.0	34.0	40.0	25.0	41.0
48	34.72052560520113	37.0	34.0	40.0	24.0	41.0
49	34.613299296464014	37.0	34.0	40.0	24.0	41.0
50	34.43075878657205	37.0	33.0	40.0	24.0	41.0
51	34.02503099846851	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	7.0
12	6.0
13	10.0
14	20.0
15	50.0
16	113.0
17	224.0
18	494.0
19	921.0
20	1606.0
21	2611.0
22	3819.0
23	5723.0
24	8119.0
25	11220.0
26	14997.0
27	18195.0
28	21005.0
29	24159.0
30	28395.0
31	34539.0
32	42437.0
33	54293.0
34	87920.0
35	124334.0
36	82927.0
37	110706.0
38	177327.0
39	323673.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.85651967926014	22.76613975626686	27.98981606021543	13.387524504257575
2	30.545717126155925	25.997099746842313	29.634620649090554	13.822562477911207
3	30.40485737314359	26.041849415669333	29.836248418294137	13.717044792892938
4	27.961932270359192	26.740130706324365	29.7568347067583	15.541102316558138
5	24.129648604920597	31.693698687258294	27.935489284234134	16.241163423586976
6	22.531373501246296	39.96314932574623	26.332722263044992	11.17275490996248
7	87.4335641161898	4.15400666329349	6.171640405899837	2.2407888146168693
8	87.53026747249973	4.05933738283935	5.9263647589577735	2.484030385703159
9	81.47168778291665	6.50243199194506	8.09774073499636	3.928139490141936
10	41.52252272867886	33.47105721940136	14.298705734483603	10.707714317436183
11	33.44224114477789	23.629943969685492	26.744114104875255	16.183700780661365
12	31.505546670599216	22.776140629224415	28.226362131609793	17.491950568566576
13	23.741648635431737	29.80734759051002	28.248906472344622	18.202097301713625
14	18.795877945278274	32.44944262083894	29.231026098040765	19.523653335842024
15	17.34617513223612	27.21957933616239	37.219774268431905	18.21447126316958
16	21.81910793908282	24.261100757099985	35.06441663975754	18.85537466405966
17	21.523743174192322	24.98489274911285	27.90726648173527	25.58409759495956
18	23.028874554304316	26.540537012976557	30.274591765213405	20.155996667505725
19	26.26034306384371	26.79776285557129	26.1671993402814	20.774694740303605
20	27.430445199877617	27.191187027342217	26.65571655830975	18.722651214470414
21	24.761059651817067	27.017782060637497	29.16618993013797	19.054968357407468
22	23.78368620311773	23.583075471842033	29.20009119440087	23.43314713063937
23	21.33296380955287	27.19415338796522	29.692507057819455	21.780375744662457
24	20.994120673245206	25.923025484427882	32.689039806016964	20.393814036309948
25	21.044803063318238	27.62707253260242	30.32663020585695	21.001494198222385
26	20.249140391068035	31.32053052088445	27.039987388729692	21.390341699317823
27	20.02539204693291	29.92108633211204	29.759885820541964	20.29363580041309
28	18.358297376804924	27.576474895690044	33.43156224653508	20.63366548096995
29	19.73384117427199	26.46807306061461	32.36214686536198	21.43593889975142
30	21.247278364128395	26.984643574820517	30.640216900288753	21.127861160762336
31	25.81174458448492	26.70733123315001	25.883530511561602	21.59739367080347
32	25.91539769996873	27.065159077444896	27.058039811949687	19.96140341063669
33	23.534766170267403	27.035834483857492	27.29322983277354	22.136169513101567
34	20.590187109552783	26.37526834969493	29.525712837645994	23.50883170310629
35	21.273721350253457	25.944891799877446	30.383499576657965	22.397887273211136
36	24.719869615737643	26.693431714802223	28.05024506376404	20.53645360569609
37	21.066245612964522	29.495032193488075	28.383155478825696	21.055566714721706
38	20.76579565843459	29.960496551817663	27.14643735851519	22.127270431232557
39	20.864363584278966	28.3779855360256	28.596902950003262	22.160747929692167
40	23.5545984098612	25.63418671290799	28.435787191593842	22.375427685636968
41	19.971828049397534	25.583504322834962	28.44850016569243	25.996167462075082
42	21.88665620812664	26.475700845073764	27.415020124637994	24.222622822161597
43	21.55772919161588	26.839122397972027	27.872602439026455	23.730545971385638
44	21.33076022737578	27.43281828837602	29.120507976543713	22.115913507704484
45	20.25541212495667	28.88328387986409	27.86989033788543	22.991413657293812
46	22.21354914878163	28.465450797823877	27.253904366228575	22.067095687165914
47	20.58866155266095	27.388407632191626	29.240433698873712	22.78249711627371
48	20.936234264516308	26.75911541431159	29.856334917369903	22.448315403802198
49	20.989628755730372	26.01769476488202	30.03821520014035	22.95446127924726
50	20.60112026727757	28.75632364519954	28.711489223211856	21.93106686431104
51	19.251595690132277	29.208820769948563	27.766321975562274	23.77326156435689
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	426.0
1	1276.5
2	2127.0
3	4501.5
4	6876.0
5	5130.5
6	3385.0
7	3237.5
8	3090.0
9	3173.0
10	3256.0
11	3239.5
12	3223.0
13	3046.0
14	2869.0
15	2907.0
16	2945.0
17	2971.5
18	2998.0
19	3041.5
20	3085.0
21	3637.0
22	4189.0
23	4911.0
24	5633.0
25	7599.5
26	11529.0
27	13492.0
28	14659.0
29	15826.0
30	19647.0
31	23468.0
32	27154.5
33	30841.0
34	34146.5
35	37452.0
36	41166.5
37	44881.0
38	49964.5
39	55048.0
40	65562.0
41	76076.0
42	82537.0
43	88998.0
44	91582.5
45	94167.0
46	99085.0
47	104003.0
48	102959.0
49	101915.0
50	96794.0
51	91673.0
52	82825.5
53	73978.0
54	68195.0
55	62412.0
56	58088.0
57	53764.0
58	48846.5
59	43929.0
60	39807.5
61	35686.0
62	32116.5
63	28547.0
64	23440.0
65	18333.0
66	14781.5
67	11230.0
68	9742.5
69	8255.0
70	6695.5
71	5136.0
72	4098.0
73	3060.0
74	2405.5
75	1393.0
76	1035.0
77	800.0
78	565.0
79	478.5
80	392.0
81	265.0
82	138.0
83	109.0
84	80.0
85	58.0
86	36.0
87	29.0
88	22.0
89	20.5
90	19.0
91	13.5
92	8.0
93	7.5
94	7.0
95	5.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1179897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.804348435977996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07177520300027	18.48789010052499
2	6.910147007912365	3.151628002245287
3	2.115929661608485	1.447571918080227
4	1.1080580377134832	1.0107416631721726
5	0.6831051086012482	0.7788883457469726
6	0.4841630124279893	0.6624613221132368
7	0.39519065575698314	0.6308445788767414
8	0.3030479231274771	0.552864834543877
9	0.2605056870899395	0.5346596212157552
>10	4.151879380363952	24.280488341895577
>50	1.9757367907871894	32.07836827567707
>100	0.5371115634394759	15.444033628437506
>500	0.0018610934284112117	0.31779838032432534
>1k	0.0014888747427289693	0.6217609871462829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3639	0.30841675163171023	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	1514	0.1283162852350671	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.475316065724381E-5	0.0	0.0	0.04466491566636749	0.0
2	8.475316065724381E-5	0.0	0.0	0.18137176380650175	0.0
3	8.475316065724381E-5	0.0	0.0	0.2700235698539788	0.0
4	8.475316065724381E-5	0.0	0.0	0.4502935425719364	0.0
5	8.475316065724381E-5	0.0	0.0	0.910418451780113	0.0
6	8.475316065724381E-5	0.0	0.0	1.1970536411229116	0.0
7	8.475316065724381E-5	0.0	0.0	1.409614568051279	0.0
8	8.475316065724381E-5	0.0	0.0	1.8807573881448973	0.0
9	8.475316065724381E-5	0.0	0.0	2.01627769203583	0.0
10	8.475316065724381E-5	0.0	0.0	2.4675882725356537	0.0
11	8.475316065724381E-5	0.0	0.0	2.8743186905297664	0.0
12	8.475316065724381E-5	0.0	0.0	3.312831543770346	0.0
13	8.475316065724381E-5	0.0	0.0	3.4456397465202473	0.0
14	8.475316065724381E-5	0.0	0.0	3.502593870481915	0.0
15	8.475316065724381E-5	0.0	0.0	3.6140442767461907	0.0
16	8.475316065724381E-5	0.0	0.0	3.8225370519630104	0.0
17	8.475316065724381E-5	0.0	0.0	4.056201515895031	0.0
18	8.475316065724381E-5	0.0	0.0	4.333598610726191	0.0
19	8.475316065724381E-5	0.0	0.0	4.500562337220961	0.0
20	8.475316065724381E-5	0.0	0.0	4.675238601335541	0.0
21	8.475316065724381E-5	0.0	0.0	4.8966138569722615	0.0
22	8.475316065724381E-5	0.0	0.0	5.1300240614223105	0.0
23	8.475316065724381E-5	0.0	0.0	5.360637411570671	0.0
24	8.475316065724381E-5	0.0	0.0	5.548111402944494	0.0
25	8.475316065724381E-5	0.0	0.0	5.722957173380388	0.0
26	8.475316065724381E-5	0.0	0.0	5.878394470025773	0.0
27	8.475316065724381E-5	0.0	0.0	6.0452734433598865	0.0
28	8.475316065724381E-5	0.0	0.0	6.218932669546579	0.0
29	8.475316065724381E-5	0.0	0.0	6.40555912931383	0.0
30	8.475316065724381E-5	0.0	0.0	6.6483769345968335	0.0
31	8.475316065724381E-5	0.0	0.0	6.852716804941449	0.0
32	8.475316065724381E-5	0.0	0.0	7.039851783672643	0.0
33	8.475316065724381E-5	0.0	0.0	7.229444604062897	0.0
34	8.475316065724381E-5	0.0	0.0	7.428529778446762	0.0
35	8.475316065724381E-5	0.0	0.0	7.660838191808268	0.0
36	8.475316065724381E-5	0.0	0.0	7.8469561326115755	0.0
37	8.475316065724381E-5	0.0	0.0	8.05655069891694	0.0
38	8.475316065724381E-5	0.0	0.0	8.278603979838918	0.0
39	8.475316065724381E-5	0.0	0.0	8.582189801313165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATG	55	1.8189894E-12	45.000004	9
TAGTACG	55	1.8189894E-12	45.000004	1
TACGAAC	25	3.8908685E-5	45.0	31
CGTAAAC	20	7.033363E-4	45.0	41
ACGTAGC	20	7.033363E-4	45.0	16
ATCGATT	25	3.8908685E-5	45.0	13
CCGATTA	30	2.1655524E-6	44.999996	12
CGGTCTA	485	0.0	43.144333	31
TCACGAC	505	0.0	42.772278	25
TACGTAG	100	0.0	42.75	1
AATACGG	90	0.0	42.5	2
CGACGGT	510	0.0	42.35294	28
GACGGTC	510	0.0	42.35294	29
CACGACG	505	0.0	41.88119	26
CGCATCG	70	0.0	41.785717	21
CTCACGA	530	0.0	41.603775	24
TACGGGA	515	0.0	41.504852	4
CGACAAT	60	3.6379788E-12	41.249996	20
TACGCGG	60	3.6379788E-12	41.249996	2
CACGTAG	50	1.0822987E-9	40.5	1
>>END_MODULE