FastQCFastQC Report
Thu 26 May 2016
SRR1548087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548087_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1119116
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44240.3953120141254347No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23110.20650227500991855No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20880.18657583306824316No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19600.17513823410620527No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14310.12786878214590802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC650.045.00000414
ACGCCTA253.8907405E-545.08
GCGGTAT253.8907405E-545.09
CATTGCG351.2118835E-745.01
GACCGTA253.8907405E-545.05
GGTCCTA351.2118835E-745.08
CGTCTAG207.0332084E-445.029
CGCCTAG253.8907405E-545.09
AACGGCC351.2118835E-745.039
CGGATAT253.8907405E-545.016
TCTCGAG253.8907405E-545.01
TTGCGTG207.0332084E-445.011
GGACGTA207.0332084E-445.08
GCGATAT1100.045.09
CCGAATA253.8907405E-545.038
CACACGG253.8907405E-545.02
GCGGAAT253.8907405E-545.022
CACGTTA207.0332084E-445.015
CACGTGA253.8907405E-545.043
CGTTTCA207.0332084E-445.020