##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548086_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1299274
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52375711358805	31.0	31.0	33.0	30.0	34.0
2	31.925198995746854	31.0	31.0	34.0	30.0	34.0
3	32.02869371664483	33.0	31.0	34.0	30.0	34.0
4	35.74588423996786	37.0	35.0	37.0	35.0	37.0
5	35.63634075645322	37.0	35.0	37.0	33.0	37.0
6	35.70455962329732	37.0	35.0	37.0	33.0	37.0
7	36.015376279368326	37.0	35.0	37.0	35.0	37.0
8	36.022499488175704	37.0	35.0	37.0	35.0	37.0
9	37.83284434230193	39.0	38.0	39.0	35.0	39.0
10	37.330362956543425	39.0	37.0	39.0	34.0	39.0
11	37.00254064962433	39.0	37.0	39.0	33.0	39.0
12	36.326669355347676	38.0	35.0	39.0	32.0	39.0
13	36.06046299702757	38.0	35.0	39.0	32.0	39.0
14	37.1135472579302	39.0	35.0	41.0	32.0	41.0
15	37.32548253871008	39.0	35.0	41.0	32.0	41.0
16	37.461644733905246	39.0	35.0	41.0	33.0	41.0
17	37.36560417587052	39.0	35.0	41.0	32.0	41.0
18	37.29127189491978	39.0	35.0	41.0	32.0	41.0
19	37.2250795444225	39.0	35.0	41.0	32.0	41.0
20	37.11355726351793	39.0	35.0	41.0	32.0	41.0
21	36.978440267410875	39.0	35.0	41.0	32.0	41.0
22	36.91910944111866	39.0	35.0	41.0	32.0	41.0
23	36.83526492487343	38.0	35.0	41.0	32.0	41.0
24	36.82990116018638	38.0	35.0	41.0	32.0	41.0
25	36.80218799113967	38.0	35.0	41.0	31.0	41.0
26	36.651106694969656	38.0	35.0	41.0	31.0	41.0
27	36.60449604933216	38.0	35.0	41.0	31.0	41.0
28	36.54810917481609	38.0	35.0	41.0	31.0	41.0
29	36.493161565612795	38.0	35.0	40.0	31.0	41.0
30	36.43578336825027	38.0	35.0	40.0	31.0	41.0
31	36.33429515252364	38.0	35.0	40.0	30.0	41.0
32	36.21734907340561	38.0	35.0	41.0	30.0	41.0
33	36.13644004266998	38.0	35.0	41.0	30.0	41.0
34	36.0432364535887	38.0	35.0	41.0	30.0	41.0
35	35.93618205243852	38.0	35.0	41.0	30.0	41.0
36	35.76817976808587	38.0	35.0	40.0	29.0	41.0
37	35.685535922368956	38.0	35.0	40.0	29.0	41.0
38	35.58897353445078	38.0	34.0	40.0	29.0	41.0
39	35.53196862247686	38.0	35.0	40.0	28.0	41.0
40	35.354733489625744	38.0	34.0	40.0	27.0	41.0
41	35.32375003271058	38.0	34.0	40.0	27.0	41.0
42	35.27513980884709	38.0	34.0	40.0	27.0	41.0
43	35.170860034142144	37.0	34.0	40.0	27.0	41.0
44	34.98331529762005	37.0	34.0	40.0	26.0	41.0
45	34.94830728545326	37.0	34.0	40.0	26.0	41.0
46	34.85811614794108	37.0	34.0	40.0	26.0	41.0
47	34.76574379230247	37.0	34.0	40.0	26.0	41.0
48	34.68208322493947	36.0	34.0	40.0	26.0	41.0
49	34.6085036720507	36.0	34.0	40.0	25.0	41.0
50	34.42620879044759	36.0	34.0	40.0	24.0	41.0
51	34.05789925758539	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	8.0
12	7.0
13	8.0
14	13.0
15	35.0
16	120.0
17	261.0
18	533.0
19	1131.0
20	1907.0
21	2913.0
22	4368.0
23	6401.0
24	8964.0
25	12590.0
26	16306.0
27	19946.0
28	22214.0
29	25600.0
30	30809.0
31	37750.0
32	47100.0
33	60949.0
34	104990.0
35	167457.0
36	89326.0
37	115576.0
38	179883.0
39	342025.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.04434014688203	23.293547011638807	28.911607559298503	12.750505282180663
2	30.04508671765925	27.080969833922637	29.498935559397015	13.375007889021099
3	29.850747417403873	26.8696210345162	29.85721256640247	13.422418981677458
4	28.288182477291162	27.021475069923667	29.856673803985917	14.833668648799254
5	23.82307350104751	32.721658403077406	27.431165404679845	16.024102691195235
6	21.48884684831683	41.72838061871476	25.45206015051483	11.330712382453585
7	87.23787284283377	4.623274228530703	6.079549040464136	2.059303888171394
8	87.72414440679948	4.498127415772193	5.764526958901663	2.013201218526654
9	82.6725540571119	6.847054585868724	7.038161311624799	3.442230045394582
10	45.63086769996167	31.67084079262727	12.584412525764389	10.113878981646673
11	38.8810982133099	21.065225656789867	24.14394500313252	15.909731126767717
12	35.256766471121566	22.354330187473927	25.925786246780895	16.463117094623613
13	22.946891879618924	34.342948446594015	26.182621987356015	16.52753768643104
14	17.485303330937125	36.69195258275006	28.26963365694996	17.55311042936286
15	16.51137481393455	25.854746573855863	40.520937077167716	17.112941535041877
16	19.894725823806215	22.487789334659205	38.73340034511581	18.88408449641877
17	19.645971519479343	23.713781696547457	27.72686900530604	28.913377778667165
18	23.041329234634112	26.515038398367086	30.536745905790465	19.906886461208337
19	28.234075337457686	26.37665342337336	25.506706052764848	19.8825651864041
20	29.248411035701476	26.54536302581288	25.724135170872348	18.4820907676133
21	23.76219334797741	27.86356072699061	29.06415428924153	19.31009163579045
22	22.882163423573473	25.202459219533367	27.40207223418617	24.51330512270699
23	21.449363259789695	28.39439563941093	27.61834686140106	22.53789423939831
24	21.55388316860031	25.343076210252804	33.99344557037238	19.10959505077451
25	20.04665682527319	28.51615594555113	31.32587891391654	20.111308315259137
26	18.544125411576005	34.01522696521288	26.580382582888596	20.860265040322517
27	19.568543663615216	32.410484624490294	28.716729496626574	19.304242215267912
28	17.234163078765526	29.62400540609602	34.50296088430924	18.638870630829217
29	18.741158523914127	26.201632604054264	33.98074617055371	21.0764627014779
30	20.43318037611774	29.400111138989928	30.751173347577186	19.415535137315146
31	28.09961563149882	27.450406919556613	24.354216277705856	20.095761171238706
32	27.762812155095844	28.096075192761493	26.084567227544	18.05654542459866
33	25.640396098128647	27.77304864101029	24.959785233907553	21.626770026953515
34	19.66305798468991	29.143198432355298	28.10415662900974	23.089586953945048
35	21.497928843338666	26.041466234220035	29.68396196645203	22.776642955989267
36	26.481481196422003	27.383446447785452	26.768795496561925	19.366276859230616
37	19.96237899011294	31.904124918993222	27.5448442745718	20.588651816322038
38	20.35205814939728	32.92284768262891	25.282349989301718	21.442744178672086
39	21.583207237272507	30.199095802732913	26.38742867170435	21.83026828829023
40	23.06588140761687	27.36097235840939	26.852457603246123	22.720688630727622
41	18.89247379690504	25.938639578718576	28.578729351930388	26.590157272445996
42	21.58028252701124	26.767410107490797	26.069404913821103	25.58290245167686
43	22.62009399095187	26.416675774317046	26.304074429258183	24.659155805472903
44	20.186350223278538	29.911011841997915	28.402861905956712	21.499776028766835
45	19.848238323863942	33.80403209792546	24.35013707655198	21.99759250165862
46	22.230722695905563	31.39891970438876	25.64724607742478	20.723111522280906
47	20.805003409596438	27.889036492687453	27.895116811388515	23.410843286327594
48	21.453134596705546	26.72946584015381	30.34009762374988	21.477301939390767
49	20.69825148506012	26.091955969256674	29.87560745462466	23.334185091058547
50	18.52242098279501	31.454643131471883	29.051300957303848	20.97163492842926
51	19.82299345634562	31.44517630615251	25.617229314217017	23.11460092328485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	635.0
1	1578.5
2	2522.0
3	5648.5
4	8775.0
5	6522.0
6	4269.0
7	4092.0
8	3915.0
9	3979.5
10	4044.0
11	3904.0
12	3764.0
13	3705.5
14	3647.0
15	3533.5
16	3420.0
17	3585.5
18	3751.0
19	3677.0
20	3603.0
21	3848.0
22	4093.0
23	4889.0
24	5685.0
25	7080.5
26	11707.5
27	14939.0
28	19502.5
29	24066.0
30	25632.5
31	27199.0
32	33329.0
33	39459.0
34	39728.0
35	39997.0
36	43400.5
37	46804.0
38	51665.0
39	56526.0
40	70465.5
41	84405.0
42	98996.0
43	113587.0
44	116116.0
45	118645.0
46	116094.0
47	113543.0
48	107412.5
49	101282.0
50	96297.5
51	91313.0
52	82477.5
53	73642.0
54	67974.0
55	62306.0
56	57704.5
57	53103.0
58	49733.5
59	46364.0
60	41510.0
61	36656.0
62	34222.0
63	31788.0
64	27022.5
65	22257.0
66	18218.0
67	14179.0
68	11691.5
69	9204.0
70	7842.0
71	6480.0
72	5673.0
73	4866.0
74	3914.0
75	2135.5
76	1309.0
77	1221.0
78	1133.0
79	708.5
80	284.0
81	232.5
82	181.0
83	144.0
84	107.0
85	71.0
86	35.0
87	32.0
88	29.0
89	23.0
90	17.0
91	11.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1299274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.390855609165868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17521139416924	15.934476587970178
2	7.3114948727508855	2.83552282729338
3	2.0916702453736997	1.2167782713008986
4	1.0207613589655604	0.7917374449246845
5	0.6026801060072725	0.58432414570519
6	0.3674734022709326	0.42753742101867476
7	0.2962625745439606	0.40213493637671754
8	0.232124410060379	0.36008727350748954
9	0.19991570431967468	0.3488882900820757
>10	2.7761791575435386	13.478216264619725
>50	1.5247893662044003	21.71143069702786
>100	1.3926833978610529	39.88184632002205
>500	0.0047749145069521815	0.6454272119852277
>1k	0.003979095422460151	1.381592308165868
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4843	0.3727466261927815	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2442	0.18795111731628586	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1706	0.13130409751907604	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	1591	0.122453000675762	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1405	0.10813731360744538	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1393	0.10721372089336045	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.040484147300723326	0.0
2	0.0	0.0	0.0	0.1468512415395059	0.0
3	0.0	0.0	0.0	0.20565331100291392	0.0
4	0.0	0.0	0.0	0.3245658729413503	0.0
5	0.0	0.0	0.0	0.6238868783643788	0.0
6	0.0	0.0	0.0	0.8016784758257304	0.0
7	0.0	0.0	0.0	0.937446604796217	0.0
8	0.0	0.0	0.0	1.2062890506544424	0.0
9	0.0	0.0	0.0	1.2848713974111696	0.0
10	0.0	0.0	0.0	1.6018176304613192	0.0
11	0.0	0.0	0.0	1.8507258669072113	0.0
12	0.0	0.0	0.0	2.162977170327429	0.0
13	0.0	0.0	0.0	2.2622633870915605	0.0
14	0.0	0.0	0.0	2.3025936022732694	0.0
15	0.0	0.0	0.0	2.385101218064858	0.0
16	0.0	0.0	0.0	2.5365704231747883	0.0
17	0.0	0.0	0.0	2.704664297138248	0.0
18	0.0	0.0	0.0	2.927173175173212	0.0
19	0.0	0.0	0.0	3.0375425045063627	0.0
20	0.0	0.0	0.0	3.163228079681422	0.0
21	0.0	0.0	0.0	3.323163551337131	0.0
22	0.0	0.0	0.0	3.4919501198361544	0.0
23	0.0	0.0	0.0	3.6690490227619423	0.0
24	0.0	0.0	0.0	3.802662102066231	0.0
25	0.0	0.0	0.0	3.919496580397976	0.0
26	0.0	0.0	0.0	4.036023194491693	0.0
27	0.0	0.0	0.0	4.1565520436797785	0.0
28	0.0	0.0	0.0	4.286701650306248	0.0
29	0.0	0.0	0.0	4.415850698159126	0.0
30	0.0	0.0	0.0	4.585329961193713	0.0
31	0.0	0.0	0.0	4.73980084262442	0.0
32	0.0	0.0	0.0	4.880802663641387	0.0
33	0.0	0.0	0.0	5.024883127038638	0.0
34	0.0	0.0	0.0	5.187358478658082	0.0
35	0.0	0.0	0.0	5.3710764627014775	0.0
36	0.0	0.0	0.0	5.532705187666343	0.0
37	0.0	0.0	0.0	5.701183891927338	0.0
38	0.0	0.0	0.0	5.868277207117205	0.0
39	0.0	0.0	0.0	6.091940576044776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGT	30	2.1657215E-6	45.000004	44
CGTAATT	30	2.1657215E-6	45.000004	36
TATAGCG	60	0.0	45.000004	1
AGCGTAT	20	7.0336263E-4	45.0	20
ACGTTAT	25	3.8910875E-5	45.0	16
TAACGCG	25	3.8910875E-5	45.0	1
CGACACG	25	3.8910875E-5	45.0	34
TACGCTA	35	1.2120836E-7	45.0	26
GTTAACG	55	1.8189894E-12	45.0	1
CGCTAAC	20	7.0336263E-4	45.0	11
TTGACGA	25	3.8910875E-5	45.0	22
CAGTCGT	20	7.0336263E-4	45.0	18
ACGACAC	25	3.8910875E-5	45.0	33
TAGTACG	110	0.0	45.0	1
GTACGAC	35	1.2120836E-7	45.0	12
AGTACGG	305	0.0	43.52459	2
CTACGAA	215	0.0	42.906975	11
GCTACGA	215	0.0	42.906975	10
CTACGGG	180	0.0	42.5	3
CACGACG	445	0.0	42.471912	26
>>END_MODULE