FastQCFastQC Report
Thu 26 May 2016
SRR1548085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548085_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1573033
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65980.4194444744642993No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19670.12504505627027532No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATT351.2123019E-745.00000422
ATCGGCC351.2123019E-745.00000419
TCACGCA253.8914637E-545.023
ACAACGT253.8914637E-545.022
CGGAACG253.8914637E-545.040
CGGCGAA207.034081E-445.025
ACACGTC207.034081E-445.016
CGAACTA253.8914637E-545.017
CAACGAT207.034081E-445.033
TCGATAG850.045.01
GTAATCG207.034081E-445.039
ATCCGGC207.034081E-445.013
CCCGTAA253.8914637E-545.018
CTAAACG207.034081E-445.01
TACGACT253.8914637E-545.028
GTCGTTC207.034081E-445.013
ACGTAAC207.034081E-445.019
CCGCGAG253.8914637E-545.01
ACCCGAT253.8914637E-545.032
ATCGGTT253.8914637E-545.010