FastQCFastQC Report
Thu 26 May 2016
SRR1548084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548084_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1647230
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51470.312463954639No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG36670.22261614953588754No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG27990.16992162600244048No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21400.129915069540987No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.0341764E-445.0000041
CGGCATG207.0341764E-445.00000430
CGTTGCA406.8175723E-945.00000436
TGCGGTA351.2123564E-745.0000044
CGTAGAT207.0341764E-445.00000432
AACGCGC207.0341764E-445.00000430
ACTCGTT207.0341764E-445.00000412
CTACCGT351.2123564E-745.00000427
ACTCGAC207.0341764E-445.00000422
CGTAATC207.0341764E-445.00000420
CGGTACC406.8175723E-945.00000441
GCGCTAA207.0341764E-445.00000436
TTCGATC207.0341764E-445.00000413
AGTCGTG207.0341764E-445.00000439
TGCGTCG406.8175723E-945.0000041
CGGACGT351.2123564E-745.00000440
CATGACG253.8915437E-545.01
CGCATCG502.1827873E-1145.021
GTTCGGT253.8915437E-545.037
CTCGATT253.8915437E-545.041