##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548084_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1647230
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.554290536233555	31.0	31.0	33.0	30.0	34.0
2	31.96795893712475	31.0	31.0	34.0	30.0	34.0
3	32.06699671569847	33.0	31.0	34.0	30.0	34.0
4	35.75794029977599	37.0	35.0	37.0	35.0	37.0
5	35.65278255009926	37.0	35.0	37.0	33.0	37.0
6	35.70644658001615	37.0	35.0	37.0	33.0	37.0
7	36.05466267612902	37.0	35.0	37.0	35.0	37.0
8	36.084512181055466	37.0	35.0	37.0	35.0	37.0
9	37.911246152631996	39.0	38.0	39.0	35.0	39.0
10	37.39623853378095	39.0	37.0	39.0	35.0	39.0
11	37.01328897603856	39.0	37.0	39.0	33.0	39.0
12	36.062123079351394	37.0	35.0	39.0	32.0	39.0
13	35.62154708207111	37.0	35.0	39.0	31.0	39.0
14	36.628997772017264	38.0	35.0	41.0	31.0	41.0
15	36.93852042519867	38.0	35.0	41.0	32.0	41.0
16	37.127278522125025	38.0	35.0	41.0	33.0	41.0
17	37.073652131153516	38.0	35.0	41.0	32.0	41.0
18	37.02566490411175	38.0	35.0	41.0	32.0	41.0
19	36.969162776297175	38.0	35.0	41.0	32.0	41.0
20	36.819852115369436	38.0	35.0	41.0	32.0	41.0
21	36.66225299442094	38.0	35.0	41.0	32.0	41.0
22	36.511352998670496	38.0	35.0	41.0	31.0	41.0
23	36.477375958427174	38.0	35.0	40.0	31.0	41.0
24	36.471449645768956	38.0	35.0	40.0	31.0	41.0
25	36.440632455698356	38.0	35.0	40.0	31.0	41.0
26	36.30557420639498	38.0	35.0	40.0	31.0	41.0
27	36.22517499074203	38.0	35.0	40.0	31.0	41.0
28	36.20936238412365	37.0	35.0	40.0	31.0	41.0
29	36.211298968571484	37.0	35.0	40.0	31.0	41.0
30	36.18169350971024	37.0	35.0	40.0	31.0	41.0
31	36.0700224012433	37.0	35.0	40.0	30.0	41.0
32	35.926262270599736	37.0	35.0	40.0	30.0	41.0
33	35.85075429660703	37.0	35.0	40.0	30.0	41.0
34	35.78747958694293	37.0	35.0	40.0	30.0	41.0
35	35.69588399919866	37.0	35.0	40.0	30.0	41.0
36	35.496780656010394	37.0	35.0	40.0	29.0	41.0
37	35.364924752463224	37.0	34.0	40.0	29.0	41.0
38	35.31993467821737	37.0	34.0	40.0	29.0	41.0
39	35.32269264158618	37.0	34.0	40.0	29.0	41.0
40	35.18397127298556	36.0	34.0	40.0	27.0	41.0
41	35.17072600669002	36.0	34.0	40.0	28.0	41.0
42	35.099977538048726	36.0	34.0	40.0	27.0	41.0
43	34.96598653497083	36.0	34.0	40.0	27.0	41.0
44	34.79716615166067	36.0	34.0	40.0	27.0	41.0
45	34.76359281946055	36.0	34.0	40.0	27.0	41.0
46	34.698464695276314	35.0	34.0	40.0	27.0	41.0
47	34.64300249509783	35.0	34.0	40.0	27.0	41.0
48	34.55605228170929	35.0	34.0	40.0	26.0	41.0
49	34.512627259095574	36.0	34.0	40.0	26.0	41.0
50	34.3335872950347	35.0	34.0	40.0	25.0	41.0
51	33.9494436113961	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	5.0
13	8.0
14	22.0
15	42.0
16	124.0
17	283.0
18	682.0
19	1284.0
20	2301.0
21	3691.0
22	5578.0
23	8153.0
24	11593.0
25	16350.0
26	21322.0
27	25688.0
28	28724.0
29	33705.0
30	40040.0
31	48877.0
32	61697.0
33	81418.0
34	153478.0
35	268270.0
36	101870.0
37	129011.0
38	202941.0
39	399933.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.863856292078218	24.461004231345957	30.82435361182106	12.850785864754771
2	28.099111842304957	27.719444157767892	30.79478882730402	13.38665517262313
3	30.036485493829034	25.12545303327404	31.247063251640633	13.590998221256289
4	27.901993042865904	27.424646224267406	29.989618936032002	14.683741796834687
5	24.05407866539585	32.3345252332704	29.248860207742695	14.362535893591058
6	21.73054157585765	41.22035174201538	26.036497635424315	11.012609046702647
7	88.50342696526897	4.715067112667933	4.913400071635412	1.8681058504276877
8	90.53216612130667	3.4139130540361697	4.371945629936317	1.6819751947208343
9	85.67109632534618	6.13933694748153	5.825051753549899	2.3645149736223843
10	51.22053386594465	30.32861227636699	10.357509273143398	8.093344584544964
11	45.186585965530014	19.589796203323154	21.69763785263746	13.525979978509376
12	42.205824323257836	21.263879361109257	22.41593462965099	14.11436168598192
13	22.258761678696963	40.03569629013556	21.932274181504706	15.773267849662767
14	15.05521390455492	41.75901361679911	25.885274066159553	17.300498412486416
15	14.588551689806522	26.565446233980683	44.79259119855758	14.053410877655214
16	17.59171457537806	21.373578674502042	43.342459765788625	17.692246984331273
17	17.68034822095275	22.993813857202696	26.082150033692926	33.24368788815163
18	21.501915336656083	26.97461799505837	31.853778767992324	19.66968790029322
19	29.327416329231497	25.977489482343085	24.46883556030427	20.226258628121148
20	31.8095226531814	25.572081615803498	24.720652246498666	17.897743484516432
21	23.794369942266712	29.092719292387827	27.400545157628258	19.712365607717196
22	23.772454362778724	24.404545813274407	25.997280282656337	25.825719541290532
23	21.00732745275402	30.363215822926975	25.034269652689666	23.595187071629343
24	20.08620532651785	25.498260716475542	37.11734244762419	17.29819150938242
25	17.681258840599064	28.06420475586287	33.738093648124426	20.51644275541363
26	16.754126624697218	36.72741511507197	25.764404485105295	20.754053775125513
27	18.520728738548957	36.26008511258294	27.8436526775253	17.3755334713428
28	15.600310824839275	30.486392307085232	37.8459595806293	16.067337287446197
29	16.009725417822647	24.94994627344087	37.2706907960637	21.76963751267279
30	18.44205120110731	32.48004225275159	30.844447952016413	18.23345859412468
31	30.19268711716032	27.732799912580514	23.00832306356732	19.06618990669184
32	29.13266514087286	28.696235498382133	25.219428980773785	16.951670379971223
33	26.583840750836252	28.978345464810623	24.12419637816213	20.313617406191
34	18.663088943256252	29.286863400982256	28.094133788238434	23.955913867523055
35	20.271789610436915	26.76796804332121	30.401279724142956	22.558962622098917
36	29.485742731737524	25.827905028441688	26.530114191703646	18.156238048117142
37	20.212781457355682	33.10836980870916	29.18378125701936	17.495067476915793
38	19.5497896468617	33.86721951397194	24.839154216472505	21.743836622693856
39	20.48651372303807	31.932638429363237	27.968953940858288	19.611893906740406
40	24.244033923617224	27.681501672504748	24.75464871329444	23.319815690583585
41	17.415115071969307	25.308669706112685	28.631338671588058	28.644876550329947
42	21.747479101279115	26.244665286572005	24.642521080844812	27.365334531304068
43	22.46013003648549	26.875117621704312	25.852795298774307	24.811957043035886
44	19.672541175185007	31.258233519302102	27.48055827055117	21.588667034961723
45	18.45722819521257	37.826168780316046	22.08094801575979	21.635655008711595
46	21.81049398080414	34.140648239772226	25.23084208034094	18.8180156990827
47	21.154605003551417	28.218767263830795	27.053356240476433	23.57327149214135
48	22.278491771033796	24.33594579991865	31.244027852819585	22.14153457622797
49	20.297226252557323	25.287846870200276	31.693024046429457	22.72190283081294
50	19.00135378787419	32.146634046247335	28.253006562532253	20.599005603346225
51	18.366166230581037	33.41464154975322	24.2829477365031	23.936244483162643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	972.0
1	1975.5
2	2979.0
3	6094.5
4	9210.0
5	7074.0
6	4938.0
7	4814.0
8	4690.0
9	4876.0
10	5062.0
11	4971.5
12	4881.0
13	4679.0
14	4477.0
15	4259.5
16	4042.0
17	4174.5
18	4307.0
19	4410.5
20	4514.0
21	4731.0
22	4948.0
23	6027.0
24	7106.0
25	8763.0
26	12561.0
27	14702.0
28	21818.0
29	28934.0
30	29506.5
31	30079.0
32	36229.0
33	42379.0
34	45173.5
35	47968.0
36	52458.5
37	56949.0
38	64096.5
39	71244.0
40	96106.5
41	120969.0
42	149588.5
43	178208.0
44	177569.0
45	176930.0
46	168697.5
47	160465.0
48	147064.5
49	133664.0
50	120251.5
51	106839.0
52	98493.5
53	90148.0
54	80745.5
55	71343.0
56	64408.5
57	57474.0
58	53850.5
59	50227.0
60	45773.0
61	41319.0
62	35335.5
63	29352.0
64	26172.5
65	22993.0
66	18957.5
67	14922.0
68	12895.0
69	10868.0
70	8871.0
71	6874.0
72	5569.5
73	4265.0
74	3396.5
75	2133.5
76	1739.0
77	1181.5
78	624.0
79	481.5
80	339.0
81	235.5
82	132.0
83	114.5
84	97.0
85	68.0
86	39.0
87	31.0
88	23.0
89	19.5
90	16.0
91	13.5
92	11.0
93	12.0
94	13.0
95	9.5
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1647230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.902456758764504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48551848829916	14.76693427961046
2	7.442314026915146	2.664714101039899
3	2.1681530607352997	1.1644579924858984
4	0.9192333584473938	0.6582614180327337
5	0.48861599656631044	0.4373713375084499
6	0.3454861127903215	0.371103011698942
7	0.26226463837978026	0.3286626943562918
8	0.2058996118243844	0.2948887117865951
9	0.15343234780269396	0.24721343747401
>10	2.2586433150237637	10.429276322994916
>50	1.5677159287209248	21.01994779236432
>100	1.691840921632011	45.58113829289907
>500	0.008161644647068983	0.9074256558469712
>1k	0.002380479688728453	0.8152523956690232
>5k	3.400685269612076E-4	0.31335255623242947
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5147	0.312463954639	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3667	0.22261614953588754	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2799	0.16992162600244048	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2140	0.129915069540987	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.070797642102196E-5	0.0	0.0	0.03909593681513814	0.0
2	6.070797642102196E-5	0.0	0.0	0.135500203371721	0.0
3	6.070797642102196E-5	0.0	0.0	0.19359773680663903	0.0
4	6.070797642102196E-5	0.0	0.0	0.34767458096319276	0.0
5	6.070797642102196E-5	0.0	0.0	0.578061351480971	0.0
6	6.070797642102196E-5	0.0	0.0	0.8418375090303115	0.0
7	6.070797642102196E-5	0.0	0.0	0.97600213692077	0.0
8	6.070797642102196E-5	0.0	0.0	1.2765066202048287	0.0
9	6.070797642102196E-5	0.0	0.0	1.3627726546991008	0.0
10	6.070797642102196E-5	0.0	0.0	1.5306909174796477	0.0
11	6.070797642102196E-5	0.0	0.0	1.7988380493313016	0.0
12	6.070797642102196E-5	0.0	0.0	2.0271607486507652	0.0
13	6.070797642102196E-5	0.0	0.0	2.118162005305877	0.0
14	6.070797642102196E-5	0.0	0.0	2.150762188643966	0.0
15	6.070797642102196E-5	0.0	0.0	2.209527509819515	0.0
16	6.070797642102196E-5	0.0	0.0	2.344299217474184	0.0
17	6.070797642102196E-5	0.0	0.0	2.511064028702731	0.0
18	6.070797642102196E-5	0.0	0.0	2.7321017708516724	0.0
19	6.070797642102196E-5	0.0	0.0	2.8409511725745644	0.0
20	6.070797642102196E-5	0.0	0.0	2.955507124081033	0.0
21	6.070797642102196E-5	0.0	0.0	3.1063057375108514	0.0
22	6.070797642102196E-5	0.0	0.0	3.2699137339655056	0.0
23	6.070797642102196E-5	0.0	0.0	3.4436599624824704	0.0
24	6.070797642102196E-5	0.0	0.0	3.5699932614146173	0.0
25	6.070797642102196E-5	0.0	0.0	3.682849389581297	0.0
26	6.070797642102196E-5	0.0	0.0	3.7892097642709275	0.0
27	6.070797642102196E-5	0.0	0.0	3.8903492529883503	0.0
28	6.070797642102196E-5	0.0	0.0	3.9997450264990317	0.0
29	6.070797642102196E-5	0.0	0.0	4.128142396629493	0.0
30	6.070797642102196E-5	0.0	0.0	4.277119770766681	0.0
31	6.070797642102196E-5	0.0	0.0	4.4173551962992414	0.0
32	6.070797642102196E-5	0.0	0.0	4.550366372637701	0.0
33	6.070797642102196E-5	0.0	0.0	4.6730571929845865	0.0
34	6.070797642102196E-5	0.0	0.0	4.816024477456093	0.0
35	6.070797642102196E-5	0.0	0.0	5.001001681610947	0.0
36	6.070797642102196E-5	0.0	0.0	5.157021181012973	0.0
37	6.070797642102196E-5	0.0	0.0	5.306484218961529	0.0
38	6.070797642102196E-5	0.0	0.0	5.444291325437249	0.0
39	6.070797642102196E-5	0.0	0.0	5.604378259259484	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.0341764E-4	45.000004	1
CGGCATG	20	7.0341764E-4	45.000004	30
CGTTGCA	40	6.8175723E-9	45.000004	36
TGCGGTA	35	1.2123564E-7	45.000004	4
CGTAGAT	20	7.0341764E-4	45.000004	32
AACGCGC	20	7.0341764E-4	45.000004	30
ACTCGTT	20	7.0341764E-4	45.000004	12
CTACCGT	35	1.2123564E-7	45.000004	27
ACTCGAC	20	7.0341764E-4	45.000004	22
CGTAATC	20	7.0341764E-4	45.000004	20
CGGTACC	40	6.8175723E-9	45.000004	41
GCGCTAA	20	7.0341764E-4	45.000004	36
TTCGATC	20	7.0341764E-4	45.000004	13
AGTCGTG	20	7.0341764E-4	45.000004	39
TGCGTCG	40	6.8175723E-9	45.000004	1
CGGACGT	35	1.2123564E-7	45.000004	40
CATGACG	25	3.8915437E-5	45.0	1
CGCATCG	50	2.1827873E-11	45.0	21
GTTCGGT	25	3.8915437E-5	45.0	37
CTCGATT	25	3.8915437E-5	45.0	41
>>END_MODULE